FastQCFastQC Report
Tue 4 Jul 2023
SRR6255560_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255560_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41779468
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG8859732.1205942593620386No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC4815981.152714534325808No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC4241471.0152044061451428RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT4180041.0005010116452417No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT4107220.9830713976539864No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT3877410.9280659102696089No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3355860.8032318649916749No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG3152420.7545380903366218No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG2943290.7044824026959846No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA2919730.6988432691388029No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA2917510.6983119076576083No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2615890.6261185518207173No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA2398150.574002043300312No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC2365470.5661800193338986No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA2346170.561560525375766No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG2165400.5182928609813796No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1998850.47842878229086117No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG1852510.4434020078953614No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG1825220.4368700913089655No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA1588060.3801053665882007No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1528410.3658280186813293No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT1484710.35536833547042773RNA PCR Primer, Index 1 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC1477030.3535301119679169No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA1416810.33911633341046854No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA1410360.33757251289078166No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1390200.3327471761966907No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG1296780.3103869106231798Illumina Small RNA Adapter 2 (100% over 21bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC1265670.3029406693258995RNA PCR Primer, Index 1 (100% over 27bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA1241820.2972321236833365No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA1199580.28712189441952685No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1188550.284481841654853No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1181760.28285664144885714Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1166640.2792376389282889No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG1157670.2770906513218407No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG1139310.2726961482611507Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC1119960.26806468670209016RNA PCR Primer, Index 1 (95% over 24bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA1118630.2677463485174105No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1092350.2614561774697562No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA1078720.25819380945683656No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC1000540.2394812686461206No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA990800.2371499799853842RNA PCR Primer, Index 1 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC970080.2321906061609018No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTG966580.23135287409595545No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA963850.23069944308529733RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA903630.21628566452784895RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA878790.21034016038691544No Hit
GGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAG864900.20701556084917117No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC849180.2032529471174693RNA PCR Primer, Index 4 (100% over 50bp)
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCC814080.19485169126615018No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGC809200.1936836534155964No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA779600.18659883366633584No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA777670.18613688427052255No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG754570.18060785264187662Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG742030.17760637832918313Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG702520.1681495800760316No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA698630.16721850072384836RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA691330.16547123098838884RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG686410.16429361905709283No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA673200.16113177889196673RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA649550.15547110365311498RNA PCR Primer, Index 1 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA644110.15416902867216978No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA642380.15375494968006773RNA PCR Primer, Index 1 (100% over 23bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG631850.151234572924672No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAAGG615360.14728765813868192Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATTGGAATTCTCGGGTGCCAAGGAA611280.146311101902973RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG603810.14452314232435895Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA597340.14297453476430097No Hit
CTCGACTGCATACTTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG589430.14108126029752222No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA583050.13955419441913428RNA PCR Primer, Index 1 (100% over 22bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC581220.13911618022517663RNA PCR Primer, Index 1 (100% over 26bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA575270.13769203571476785No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGC572630.13706014638577974No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG550050.131655577806783No Hit
CTCGACTGCATCATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG542660.1298867663896534No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG536540.12842193203609006Illumina Small RNA Adapter 2 (100% over 21bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCC535690.1282184828203174No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG534490.12793126039805008No Hit
CGCCGCTGGTGTCGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA533180.12761770925374158No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTGC509300.12190198305062189No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG507480.1214663623768498Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT506830.12131078356478833RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA497850.11916140243815455No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGGT492010.11776358664978694No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG481510.11525039045494788No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA474670.11361322264802415No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGGA469270.1123207217478212RNA PCR Primer, Index 1 (100% over 22bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAA466920.11175824450421437No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA466740.11171516114087426RNA PCR Primer, Index 1 (100% over 23bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA461730.1105160075279082RNA PCR Primer, Index 1 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA459270.1099272015622602No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAA459200.10991044692096126No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG448720.10740203776649333Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA441710.10572418011641509No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGG435970.10435029952990307Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCTGGAATTCTCGGGTGCCAAGGAA430600.10306497919025681RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA426000.10196395990489875No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG423810.10143977898426089Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)