FastQCFastQC Report
Fri 7 Jul 2023
SRR6255561_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255561_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33774909
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG6710351.9867855158395837No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC3753571.1113486641814492No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC3444561.0198576700828417RNA PCR Primer, Index 1 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG3290430.9742232022001895No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT3193810.9456161673152103No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT2906470.8605411786601704No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT2863160.8477180501063674No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC2523160.7470516056756807No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG2481380.7346814761218157No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA2351890.696342364682611No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA2270660.672291966797009No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA2245820.6649373947980142No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2068670.612487216471849No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1979580.5861096472532317No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1753410.5191457362623834No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1644960.48703610126677177No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1577700.46712190993616004No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA1454850.430748754941131No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG1424920.4218871470534532No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG1357760.4020025635006152No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA1195700.3540201988405061No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA1185870.35110975428534835No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT1180820.3496145615077749RNA PCR Primer, Index 1 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC1158670.3430564387308934No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA1154020.3416796770644149No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1091920.3232932470669277No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA1030200.3050193266249807No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1013070.2999475142923405No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG970740.28741454196071997Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA969470.2870385231829936No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC929260.27513323574017623No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC912970.2703101287408354RNA PCR Primer, Index 1 (100% over 27bp)
GGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAG900000.2664699999635824No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG896440.2654159630748376Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG890270.26358916318619835No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA872140.2582212730758209No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG871260.25796072463141206Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC866030.25641223785384587RNA PCR Primer, Index 4 (100% over 50bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG846720.25069497596573836No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA843490.24973864474364685No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA828980.2454425561886784RNA PCR Primer, Index 1 (100% over 22bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA748140.22150762863639398RNA PCR Primer, Index 1 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC746670.22107239430312012No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC740370.21920710430337503No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG739170.21885181097009024Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA734060.21733885352585258RNA PCR Primer, Index 1 (100% over 22bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA717730.21250390341540226No Hit
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTG716100.2120212966376904No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAAGG678270.20082067430588785Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA673420.1993846970838619No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA612940.18147791308630912RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA598440.17718478530911808No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCC592690.17548233808712851No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGC583620.17279691264305108No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG575280.1703276239767219Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG573100.16968217442125455Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG569530.16862517675473235No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG568060.1681899424214585No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA564950.16726914053269543No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA557730.16513145897743203RNA PCR Primer, Index 1 (100% over 22bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG552670.16363330542208124Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGGGGGATGCGTGCATTTATCAGATTGGAATTCTCGGGTGCCAAGGAA537370.15910331542270034RNA PCR Primer, Index 1 (100% over 23bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA533120.15784498486731674RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG523880.15510922620102396No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG511850.15154741053484408No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCC507830.15035717786834008No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG494330.14636012786888633Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA484490.14344672253595117No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA483790.14323946809153507RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA479490.1419663336472646RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA470090.13918320253653385No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA450780.13346594064842632RNA PCR Primer, Index 1 (100% over 23bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAA442160.1309137502043307No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCA434900.12876422553795777No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG434650.12869020609352344Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC426950.12641040720494612RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGC425550.12599589831611388No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA422220.12500995931624864RNA PCR Primer, Index 1 (100% over 23bp)
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG414560.12274200353878083No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG409820.12133859487230596No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT398080.1178626417616699RNA PCR Primer, Index 1 (100% over 30bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGGT392590.11623717476189202No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA388150.11492258942873836No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAG380350.11261318276238731No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTGC374040.11074493198486486No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA370290.10963464031834994No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGTGGAATTCTCGGGTG366370.10847401542961965No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA363620.10765980154084204No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA360330.1066857056520863No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGGA351970.10421049543020233RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)