FastQCFastQC Report
Mon 10 Jul 2023
SRR6255562.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255562.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98862335
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG19939592.0169046179214765No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC11186451.131517882922753No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC10088781.0204877317534529RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT9473670.958268889764742No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG9441730.9550381345939281No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT8626680.8725952102992509No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT8493410.8591148489462646No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC7542800.7629599280656278No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG7365410.7450167953245288No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA6856540.6935442097336665No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA6847610.6926409334758278No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA6685280.6762211311314871No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC6152270.6223067662725141No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA5938630.600696918598979No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC5269280.5329916595637763No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG4907890.49643678757941534No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC4677760.4731589639269596No Hit
CGGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAA4244390.4293232604712401No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG4241170.428997555034483No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG4043640.4090172460523009No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA3503320.3543634691614354No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT3491130.35313044143656935RNA PCR Primer, Index 1 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC3459130.3498936172203499No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA3398140.34372443256574914No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA3352530.33911094655006885No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC3289500.3327354143516841No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA3064310.30995727543760726No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA3022760.3057544614943598No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG2908350.29418180341380773Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA2859560.28924665799164057No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC2770480.28023614857973966No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC2712410.27436232413486894RNA PCR Primer, Index 1 (100% over 27bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG2664260.26949191519702625No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG2662600.2693240049408099Illumina Small RNA Adapter 2 (100% over 21bp)
GGGCCGCCGGTGAAATACCACTACTCTCATTGGAATTCTCGGGTGCCAAG2632620.26629150525323925No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG2602470.26324180993702Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA2594510.26243664991323545No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG2530770.2559893006775533No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTCGTATGC2509610.2538489506645782RNA PCR Primer, Index 4 (100% over 50bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA2503020.2531823671775505No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA2395490.24230562630348554RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGTGGAATTCTCGGGTGC2234680.22603957310941525No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC2227300.22529308052454963No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2226210.22518282619968466RNA PCR Primer, Index 1 (100% over 23bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA2195540.2220805324899518RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAGG2156630.2181447565445425Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTG2120950.2145356975434578No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA2061050.208476767213722No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA1999770.20227824883966172No Hit
CCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAAGG1954410.19769005051317065Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1797030.18177094441477637RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA1779500.17999777164882863No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCC1766800.17871315703801655No Hit
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGC1758670.1778908013855833No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG1700830.17204024161476664No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA1673060.1692312850996287No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAAG1667130.16863146111206054No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA1655840.16748946906827558RNA PCR Primer, Index 1 (100% over 22bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1646940.16658922733313958Illumina Small RNA Adapter 2 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG1639480.1658346426877334Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG1635210.16540272895638164Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1594450.16127982411097208RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGGGGATGCGTGCATTTATCAGATTGGAATTCTCGGGTGCCAAGGAA1592290.16106133847637727RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG1558310.15762423576177925No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCC1482320.1499377897558256No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG1461450.14782677346231No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA1447140.14637930613311936RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1430570.14470323809365823No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG1419630.1435966488147382Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1414840.14311213668987285RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1378040.13938978884122047No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA1341300.1356735100379735RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG1292730.13076061778229292Illumina Small RNA Adapter 2 (100% over 21bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAA1280750.12954883171634576No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC1255220.12696645289634317RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGTGGAATTCTCGGGTGC1253180.1267601053525592No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG1250220.1264606991125589No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCA1241780.12560698672553103No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1225950.12400577024606996RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG1218650.12326736972174487No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1179160.11927292633741658RNA PCR Primer, Index 1 (100% over 30bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGGT1168630.11820780886876686No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC1150170.11634056589903527RNA PCR Primer, Index 1 (95% over 24bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA1144290.11574579944930494No Hit
GGGGGTGGGGGCCGGGCCGCCCCTCCCACGGCGCTGGAATTCTCGGGTGC1110800.11235826060551775No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAG1079660.1092084260401092No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGTGGAATTCTCGGGTG1073210.10855600365902748No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1067940.10802293917091883No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA1066030.10782974122551324No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA1061490.10737051678983711No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGGA1059540.10717327281416122RNA PCR Primer, Index 1 (100% over 22bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAA1008030.10196299733361547No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA1001610.10131360947523646RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAGG988650.10000269566766758Illumina Small RNA Adapter 2 (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)