FastQCFastQC Report
Tue 4 Jul 2023
SRR6255563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39160588
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG9030312.30596895021086No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA4304271.0991331386546086No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC4222001.0781247717730897No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA3109430.7940202532198956No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC2981710.7614058297592468No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2968040.757915075228186No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA2866800.7320625522783264No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2779100.7096675872180469No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC2771080.7076196097974832RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT2714520.6931765171656769No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA2528860.6457666059559677RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG2282390.582828327296822No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA2255230.5758927828152121No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA2062170.5265932166289229No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC1936760.4945686719515039RNA PCR Primer, Index 1 (100% over 27bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA1889630.4825336126209341RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1885090.48137428375692415No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1855360.4737824672091236Illumina Small RNA Adapter 2 (100% over 21bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1839780.46980397740708085No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA1823800.4657233440927905No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG1625670.41512910888876337No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA1550370.39590059270815847RNA PCR Primer, Index 1 (100% over 22bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC1516280.38719541187685946RNA PCR Primer, Index 1 (100% over 26bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT1516060.38713923294512326No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG1514660.3867817306522568No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1386880.3541519856647709RNA PCR Primer, Index 1 (100% over 22bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA1278930.32658600529695825No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1244830.317878270877853No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1214150.31004386348846447No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG1178500.30094032295939993Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA1116370.2850748819195463RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA1114760.28466375428274976No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1105660.2823399893791176No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1084580.2769570262836707Illumina Small RNA Adapter 2 (100% over 21bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1080590.2759381447490012RNA PCR Primer, Index 5 (100% over 50bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1007480.2572688642979518No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG993900.25380109205714685Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA968590.24733796131968191No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG951180.24289216494910648No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA921500.23531311634033686RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT918870.2346415227473091RNA PCR Primer, Index 1 (100% over 25bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA901260.2301446546206099No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA899050.22958031171544208No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG832780.21265768532382606Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG811680.2072696150527668No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG773480.19751490963312401No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA771520.1970144064231109No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG750810.19172592607649303No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC741530.18935619659234945No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC732640.1870860570326472No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC720850.1840753770091501No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG712770.18201207806174924No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC694190.17726751191784965No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA693950.1772062258105011RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA688850.1759038960293446No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC682080.17417511708455452RNA PCR Primer, Index 1 (95% over 24bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG677020.17288300165462275No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC669610.17099079309023654No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC665970.17006128712878366No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC661740.1689811194867656No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA660000.1685367952084887No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC648070.16549036495570496No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG601670.15364171753498695No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG599960.1532050540201286Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT594450.15179802713891835RNA PCR Primer, Index 1 (100% over 30bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA593350.15151713248023754No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG591850.15113409430930913No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA589590.1505569834651104No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG580320.14818980756877298No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA575640.1469947284754764No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC564070.1440402273837155No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA542260.13847085237841678No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG539160.13767924015849814No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA533930.13634371373586116No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCAAG529080.13510522364985939No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG528130.1348626328082714No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG528090.13485241845704665Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGTGGAATTCTCGGGTGC525100.1340888957029961No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC517910.13225286607034603No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA505780.12915536406143852No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG504970.12894852344913718Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA504820.12891021963204435No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG501490.12805987489258333No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA494260.12621363090870852RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG487650.12452570936881745Illumina Small RNA Adapter 2 (100% over 21bp)
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG487000.12435972616141515Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC483600.1234915063073108No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGTGGAATTCTCGGGTGCCAA480470.12269223332397358No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG479110.12234494538233184No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTTGGAATTCTCGGGTGCCAAG476050.12156354751363796No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCCA469600.11991648337864588No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG466960.11924233619781195No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC463890.11845838474131186No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCC461240.11778168397267172No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC457020.11670406991845986RNA PCR Primer, Index 1 (100% over 26bp)
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAA452280.11549366929832616No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG450670.1150825416615297Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA447920.11438030501482767RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC446310.1139691773780312No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG436910.11156880484021334No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA436690.11151262590847716No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG435660.11124960636443967Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAA421400.10760819015281384No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGTGGAATTCTCGGGTGCCAA415160.10601475136175177No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT411580.10510056692713604No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA411500.10508013822468651RNA PCR Primer, Index 1 (100% over 23bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG407830.10414297149981507No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA406830.10388761271919614RNA PCR Primer, Index 1 (100% over 23bp)
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA404940.10340498462382638No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGGTGGAATTCTCGGGTGCCAA403260.10297598187238659No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)