FastQCFastQC Report
Fri 7 Jul 2023
SRR6255564_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255564_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33926943
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG7477112.2038855667013677No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA3894681.147960781494519No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3478961.0254269003841578No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2539480.7485142413214182No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC2539450.7485053987917508No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA2484700.7323677821488367No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC2383730.7026067747984249RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA2283430.673043250610584No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT2252310.6638705998356528No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2213380.6523959438373212No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA2208890.6510725118971079RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA1888040.5565016571047972No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1837920.5417287375405441No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA1701730.5015866003606633No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1600460.47173716771357793No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1571390.4631687564659156No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA1557420.45905108515081955RNA PCR Primer, Index 1 (100% over 23bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1535600.45261961857276684Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA1515770.44677470646264833No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC1498940.4418140473192648RNA PCR Primer, Index 1 (100% over 27bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG1367710.4031338750443858No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG1303500.3842079140463672No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA1245360.3670710915510425RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1235200.3640764215036999RNA PCR Primer, Index 1 (100% over 22bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC1217570.35887996156918706RNA PCR Primer, Index 1 (100% over 26bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCGTATGC1193430.3517646726968593RNA PCR Primer, Index 5 (100% over 50bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT1150560.3391286978022158No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA1079150.31808052968403316No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1063850.31357083955368453No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1063300.31340872650978313No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG984390.2901499259747629No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA971270.28628279300024173No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG965680.2846351349722255Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA894940.2637844500166136RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA893060.2632303181574597No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC884160.2606070343561458No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG856530.2524630645325162Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA848210.2500107363047711No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA837420.24683037313441414RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG795040.23433882622433738Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG790340.2329534965764525No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT784170.2311348829748675RNA PCR Primer, Index 1 (100% over 25bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA750640.22125188231665904No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA739380.2179329861815136No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA738440.21765592025193664No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG709070.20899908370760079Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC699440.20616063168438134No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG653300.1925608210559967No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA630560.18585818356814524No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG615330.18136912600702043No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG600170.17690070101511945No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG596260.1757482246484748No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC589900.17387360835899657No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG589640.17379697310187953Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG578030.17037491412061498No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC573740.1691104323781839No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC562820.16589175157926844No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA561230.16542309750689888No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA560460.16519613924543688RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC549290.16190377069929346No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC537220.15834612626312958No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGTGGAATTCTCGGGTGCCAAGG533710.1573115502920496Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC533200.15716122728770465No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA532230.15687531882846031No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC517070.15240689383655934No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG503570.14842775548625176No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT496670.1463939736627612RNA PCR Primer, Index 1 (100% over 30bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA494080.14563056860147994No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA478380.14100297807556667No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG478350.14099413554589932No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG476040.14031326076151335No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG475210.14006861744071666No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA471020.13883361079717674No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG469050.13825295134902077Illumina Small RNA Adapter 2 (100% over 21bp)
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGTGGAATTCTCGGGTGC455830.13435634327560841No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC454260.1338935842230171No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCAAG450690.1328413231926024No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA446630.131644634177621No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG430140.1267841903704675Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG429790.12668102752434843No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG424100.125003894397441Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG423590.12485357139309604No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA419250.12357435210121938No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGTGGAATTCTCGGGTGCCAA414390.12214186229510865No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG409080.12057673454398765Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA409070.12057378703409855No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC407010.11996659999694047No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG405230.11944194323667771No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA404850.11932993786089127RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA402800.11872569833362234No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA401190.11825114924147455RNA PCR Primer, Index 1 (100% over 23bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA399360.11771175493176618No Hit
TTCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCC399020.11761153959553622No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTTGGAATTCTCGGGTGCCAAG394580.11630284520476837No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC391010.11525058417435369No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGTGGAATTCTCGGGTGCCAA389930.1149322531063291No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCCA388910.11463160709763917No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA388350.11446654654384864RNA PCR Primer, Index 1 (100% over 22bp)
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAA386940.11405094764948319No Hit
TGCGAGAGGTAGCGGGATCGATGCCCGCATCCTGGAATTCTCGGGTGCCA385810.11371787903201298No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG385010.11348207824088366No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAAGG381580.1124710823489166Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC375120.1105669909605472No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA373970.11022802732329877RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA370530.10921408392144262No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA368150.10851257656783282RNA PCR Primer, Index 1 (100% over 23bp)
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG363380.10710661435072413No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC356650.10512294019534858No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC354300.10443027537140614RNA PCR Primer, Index 1 (100% over 26bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG354090.1043683776637347Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGCC346360.10208995251944745No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA342690.10100821639014161No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGGTGGAATTCTCGGGTGCCAA341910.1007783106187905No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA339640.10010922587396098No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)