FastQCFastQC Report
Tue 4 Jul 2023
SRR6255566_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255566_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36704618
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length60
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC9330122.5419471740585884RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC7496362.042347913823814RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG6279751.7108882593465486Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC6139951.6728004089294706RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG5491461.4961223680355427No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA3913031.0660865616419166RNA PCR Primer, Index 1 (100% over 23bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT3510580.956440957919791RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA3364960.9167674759617441No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3291380.8967209521156165No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2702480.7362779255732889RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2598030.7078210158732615No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC2353510.6412026955300284RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC2232310.6081823273572824RNA PCR Primer, Index 1 (95% over 24bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA2071060.5642505256423047RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1695920.46204540257032506No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA1662950.4530628816243231RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA1618310.4409009242379256RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1613740.43965584929939877No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT1605020.4372801264407655RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1573310.4286408865500248RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG1553630.423279163401183No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG1489910.4059189500351155No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT1485890.40482372000166295No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT1478570.40282942053776444RNA PCR Primer, Index 1 (100% over 25bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1445900.3939286331763486Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT1425040.38824542459480166No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1411700.38461100453354397No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1270550.34615535298582867No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1262970.34409021774862225RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA1210960.32992033863422854No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1123730.30615493668943783Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC981920.2675194712556333RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG972890.2650592903595945Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCTGGAATTCTCGGGTGCCAAGG888500.24206763301555131Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA884450.24096422962364025No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA870700.23721810699678172RNA PCR Primer, Index 1 (100% over 22bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA806170.21963721295233202No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA802630.2186727566542172No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA790410.21534347530874726No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA768000.2092379765401727No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCACGGAACT763220.20793568809243565RNA PCR Primer, Index 1 (96% over 25bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG761720.20752702016950567Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA750210.2043911749742226No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA745300.20305346863983162RNA PCR Primer, Index 1 (100% over 22bp)
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA734130.20001025484041274No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG733950.19996121468966113No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG727990.19833744080921917No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA719370.19598896247878128No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG704670.19198401683406702No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA691260.18833052560307262RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGATGGAATTCTCGGGTGCCAAGGA685060.18664136485496186RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC678760.18492495957865576No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT677180.18449449603316945No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACTGGAATTCTCGGGTGCCAAGG674830.18385424962057909Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC653280.1779830537944844No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC646440.17611952806592346No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC636360.17337327962383373No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA621060.16920486680994745No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA608880.16588648327575567No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA596530.16252178404363177No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA590310.16082717438988195No Hit
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT578280.15754965764798315RNA PCR Primer, Index 1 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA576700.15711919410249686No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC576010.15693120685794903No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG569950.15528018844931177No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA568520.15489059169611846RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC561520.15298347472244503RNA PCR Primer, Index 1 (100% over 26bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG553830.15088837050422374Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC553080.15068403654275872No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG550180.14989394522509403No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG549350.14966781564107273Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG548290.1493790236422022No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC536620.1461995872018066No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA522430.14233358865088855RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG506130.13789273055504894No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA505920.13783551704583877No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA503990.13730969765166878No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG503460.1371653016522335No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG503160.13708356806764752No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC500990.13649236180580873No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC498440.13579762633682768No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA493200.13437001305939214No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA492890.1342855550219866No Hit
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGTGGAATTCTCGGGTGC486460.13253373185902656No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC483710.13178450733365488No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG478420.13034327179212163No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA475250.1294796202483295No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA474590.1292998063622403No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA461540.1257443954327491No Hit
GCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAAC457580.12466551211621382RNA PCR Primer, Index 1 (100% over 24bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAAC456100.12426229309892287RNA PCR Primer, Index 1 (100% over 24bp)
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA451110.12290279114197565RNA PCR Primer, Index 1 (100% over 28bp)
CGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA447970.12204731295664213RNA PCR Primer, Index 1 (100% over 28bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAAG446840.12173944978803485No Hit
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG445530.12138254646867595RNA PCR Primer, Index 1 (100% over 29bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG442720.12061697522638705No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG437340.11915121960947803No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC432450.11781896218072613No Hit
CCGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCC423910.11549227947284454RNA PCR Primer, Index 1 (100% over 27bp)
GGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGGAA421100.11472670823055563RNA PCR Primer, Index 1 (100% over 23bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG420950.11468584143826263Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGACCGGGGTTCAATTCCCCGACGGTGGAATTCTCGGGTGCCAA420250.11449512974089526No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGTGGAATTCTCGGGTGCCAA418060.11389847457341742No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA416890.11357971359353201No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA406210.11066999798227024No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG394200.1073979301460105Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGGA389100.10600845920804843RNA PCR Primer, Index 1 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC388550.10585861430297408No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTTGGAATTCTCGGGTGCCAAG372630.10152128541427675No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGA367940.10024351704191554RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)