FastQCFastQC Report
Fri 7 Jul 2023
SRR6255567_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255567_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37596260
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC9665762.5709365772020942RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC7114771.8924142986563024RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG6101921.6230125017754424Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC5991311.5935920221851856RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG5398251.4358476082461393No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT3590410.9549912677484409RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA3497200.9301989080828785RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA3236980.8609845766573591No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3222840.8572235642587853No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2712710.7215371954550799RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2496450.6640155164370073No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC2208350.5873855537758277RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA2019040.5370321409629575RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA1899650.5052763226980556RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA1755230.4668629273230901RNA PCR Primer, Index 1 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT1686760.44865100943551295RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1633190.4344022517133353No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1616210.42988584502820226No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1608440.4278191500963128RNA PCR Primer, Index 1 (100% over 30bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT1607260.4275052890899254RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG1492730.3970421525970934No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG1443150.3838546706507509No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1435460.3818092544311588Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT1408620.3746702464553655No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT1385730.3685818748992586No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1361540.3621477242683182No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1293690.3441007164010463No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1252800.3332246345780139RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA1198620.3188136266745682No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1068840.2842942356500354Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC1056050.28089230152148115RNA PCR Primer, Index 1 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1045110.2779824376148053RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG992710.2640448810599778Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA885100.2354223531808749No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA853300.22696406504263988No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCTGGAATTCTCGGGTGCCAAGG849650.2259932237940689Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA804800.21406384571231288No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC787660.20950488160258493RNA PCR Primer, Index 1 (95% over 24bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG787590.20948626272932466No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA768040.20428627741163616No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA754620.2007167734237395No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA754040.200562502759583No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG750650.1996608173259787Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG749680.19940281293937215No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA737020.19603545671830125No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA705890.18775537779555734No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA702200.1867738971908376RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA674990.17953647517066856RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG668410.17778630108420357No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGATGGAATTCTCGGGTGCCAAGGA659900.1755227780635627RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA654110.17398273126103503No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT653100.17371408751827974No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACTGGAATTCTCGGGTGCCAAGG648000.17235756960931753Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG647720.17228309411627646No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC646610.1719878519831494No Hit
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT645800.17177240502113775RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC632320.16818694199901799No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA618290.16445518782985327No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC615830.1638008674267068No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC607760.16165437732370186No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA601950.16010901084309984RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA599690.15950788722069698No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA590880.15716456902894063No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC583430.15518298894624094No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA575530.15308171610686808No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG564210.1500707783167794No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA562880.1497170197248343No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG546800.14543999855304757Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAAC544630.14486281348197932RNA PCR Primer, Index 1 (100% over 24bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG543370.14452767376329456No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG540520.143769619637698No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG536330.14265514708111923Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC533780.14197688812663814No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG527680.14035438631395783Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG525140.1396787871985139RNA PCR Primer, Index 1 (100% over 29bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAAC520360.13840738413874146RNA PCR Primer, Index 1 (100% over 24bp)
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA518040.13779030148211552No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG515530.13712268188378313No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA512940.13643378357315328RNA PCR Primer, Index 1 (100% over 28bp)
CCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC505970.13457987576423824RNA PCR Primer, Index 1 (100% over 26bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC498050.13247328324679103No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG496290.1320051515762472No Hit
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGTGGAATTCTCGGGTGC496110.13195727447357794No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA495240.13172586847734322RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG492250.13093057660522614No Hit
CGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA484660.1289117587760059RNA PCR Primer, Index 1 (100% over 28bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC484200.12878940618029558No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA483290.12854736082791213No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC481830.12815902432848372No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA480570.12782388460979896No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG475710.12653120283772906No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA475700.1265285429986919No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA459780.1222940792515No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG451800.12017152769982972No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC451790.12016886786079253No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAAG448440.11927782178333696No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGGA445340.11845327168181091RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGGA444440.11821388616846462RNA PCR Primer, Index 1 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC442350.11765797980969385No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA441020.11730422121774878No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC437040.11624560528095082RNA PCR Primer, Index 6 (100% over 50bp)
GCGTTGGTGGTATAGTGGTGAGCATAGTGGAATTCTCGGGTGCCAAGGAA433870.11540243630616451RNA PCR Primer, Index 1 (100% over 23bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGTGGAATTCTCGGGTGCCAA428200.11389430757208296No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG427900.11381451240096754No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA419500.11158024760973566No Hit
CCGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCC414830.1103381027793722RNA PCR Primer, Index 1 (100% over 27bp)
GGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGGAA407760.10845759658008536RNA PCR Primer, Index 1 (100% over 23bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA405740.10792030909457483No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGTGGAATTCTCGGGTGCCAA403800.10740430032136175No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA402470.1070505417294167RNA PCR Primer, Index 1 (100% over 23bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACTGGAATTCTCGGGTGCCAAG394680.10497852711945284No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG392020.10427100993556274Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC388010.10320441448165323No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG383250.10193833109995516Illumina Small RNA Adapter 2 (100% over 21bp)
GGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA382240.10166968735719989RNA PCR Primer, Index 1 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)