FastQCFastQC Report
Tue 4 Jul 2023
SRR6255568.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255568.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110537980
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC28448292.573621301927175RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC21316071.9283932997509092RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG18218341.6481520650187382Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC17859051.6156483047727124RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG16069361.4537410580508165No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT10742630.9718496755594774RNA PCR Primer, Index 1 (100% over 25bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA10517220.9514575895090539RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA9684150.8760925430336252No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC9581980.8668495660948392No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA8099010.7326902481843798RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA7469310.6757234029425905No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC6629480.5997468019589285RNA PCR Primer, Index 1 (100% over 26bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA6010580.5437569964640208RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA5623700.5087572615312854RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA5242000.47422614381048034RNA PCR Primer, Index 1 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT4997770.45213147553447236RNA PCR Primer, Index 1 (100% over 25bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA4887120.44212134146109777No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC4837000.4375871533024215No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT4833260.43724880805674216RNA PCR Primer, Index 1 (100% over 30bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT4782100.4326205345891068RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG4453550.40289771895596427No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG4302980.389276156484857No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG4273920.38664719583259977Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT4187830.37885892251694847No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT4098870.3708110099352277No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG4070050.36820376127734555No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC3860180.34921752686271273No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA3740210.3383642436744366RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA3566820.3226782324048259No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG3198540.28936117703616443Illumina Small RNA Adapter 2 (100% over 21bp)
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC3104530.28085640790613325RNA PCR Primer, Index 1 (100% over 24bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA3029430.27406236299957715RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG2956760.2674881520360694Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC2860390.258769881628016RNA PCR Primer, Index 1 (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA2650250.2397592212197111No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCTGGAATTCTCGGGTGCCAAGG2543140.2300693390633699Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA2530050.22888513070349215No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA2401620.21726649971349216No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG2379880.21529975488967681No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA2313390.20928462778132909No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA2262780.20470611096747018No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA2244850.2030840440543603No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG2231230.2018518883735708Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG2218980.20074367199400603No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA2213660.20026238945202363No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA2107400.19064940394242774No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA2085630.18867994511931555RNA PCR Primer, Index 1 (100% over 22bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA2019920.18273538199268705RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG2007720.18163168894528378No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGATGGAATTCTCGGGTGCCAAGGA1958930.17721782142210304RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCAATTCCCCGACTGGAATTCTCGGGTGCCAAGG1954640.17682971952264734Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT1947080.17614579169983022No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1936420.17518141728300082No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC1935550.17510271130339095No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG1907110.17252983996993612No Hit
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT1904410.17228558003321573RNA PCR Primer, Index 1 (100% over 25bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1877320.16983483866812113No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC1872390.16938883811699834No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1840670.1665192361937499No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1828000.16537302382402863No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1792730.16218226531731447RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA1779590.16099353362527521No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA1763510.15953883000214042No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC1737730.15720659993967684No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA1722530.15583150696258427No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1673560.15140135544362218No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA1654130.14964358856566767No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG1637750.14816174494956394No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG1635440.14795276700370316Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1611620.14579785156196992No Hit
GCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAAC1609320.14558977828254144RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC1597480.1445186532267009No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG1574370.1424279691016608Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG1561990.1413079920584762Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG1550770.14029295632143812RNA PCR Primer, Index 1 (100% over 29bp)
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA1544340.13971125580547067No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG1532610.1386500820803854No Hit
CCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC1522570.13774179698235847RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAAC1520990.13759885968605542RNA PCR Primer, Index 1 (100% over 24bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC1507630.1363902253325056No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1503000.13597136477435176RNA PCR Primer, Index 1 (100% over 28bp)
CTCCCCGCGTCCGGCGCTCCGGCGGCGGGCGCGGTGGAATTCTCGGGTGC1488820.13468854777335357No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG1485650.13440176851431518No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA1479970.13388791798077004RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG1466540.13267295096219417No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1449510.1311323040279911No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1444090.1306419748216857No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC1443410.13058045750428948No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA1438220.13011093562592693No Hit
CGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA1420050.12846715671844192RNA PCR Primer, Index 1 (100% over 28bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA1417600.12824551344252896No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG1414820.12799401617435022No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA1383290.1251416029133154No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC1347860.12193636974368448No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAAG1329440.12026997417539202No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA1323780.1197579329747115No Hit
GGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGGA1319550.11937525907384954RNA PCR Primer, Index 1 (100% over 22bp)
GCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGGA1315020.11896544518001867RNA PCR Primer, Index 1 (100% over 22bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC1294320.11709278566516233No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG1292210.1169019010479475No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGGTGGAATTCTCGGGTGCCAA1273110.1151739881622588No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGTGGAATTCTCGGGTGCCAAGGAA1269660.11486187824311608RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG1269560.1148528315787931No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAATATCTCGTATGC1266180.11454705432467646RNA PCR Primer, Index 6 (100% over 50bp)
CCGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCC1246030.11272415146359649RNA PCR Primer, Index 1 (100% over 27bp)
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1244030.11254321817713694No Hit
GGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGGAA1219690.11034126008092422RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCAATTCCCCGACGGTGGAATTCTCGGGTGCCAA1207420.10923123436849488No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA1194810.10809044999736742No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACTGGAATTCTCGGGTGCCAAG1168120.10567589528956473No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1167190.10559176131136104No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA1155310.10451701758979132RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG1153980.10439669695429571Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG1127150.10196947691644084Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAG1114160.10079431522088605No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGTGGAATTCTCGGGTGCCAA1113830.10076446122862025No Hit
GGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1112990.10068846924830724RNA PCR Primer, Index 1 (100% over 28bp)
AGCAGTTGACCCGGGTTCGATTCCCGGCCAATGGAATTCTCGGGTGCCAA1111190.10052562929049363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)