FastQCFastQC Report
Tue 4 Jul 2023
SRR6255570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25713585
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG5945082.3120385586062775No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC5746262.2347175627202507RNA PCR Primer, Index 1 (100% over 24bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC3850631.4975080293160212No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT3703221.4401803560258128No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG3434391.3356325070969295No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC2680651.042503408217874RNA PCR Primer, Index 1 (100% over 27bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA2468130.9598544893681686No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1833820.7131716561498522No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA1799580.69985573773552No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT1745990.6790146142593496RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC1672580.6504655029627335No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC1658760.6450909120606869No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1540480.5990918808093076No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1420980.5526183921845204No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG1276050.49625518962058385No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1274090.49549294662724No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC1248390.4854982298267628RNA PCR Primer, Index 1 (100% over 26bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1231200.4788130476555486RNA PCR Primer, Index 1 (100% over 30bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA1114060.43325736181866514No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA1038370.4038215596930572No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA963770.37480965800762517RNA PCR Primer, Index 1 (100% over 23bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT899890.3499667588164No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA898340.34936396461248015RNA PCR Primer, Index 1 (100% over 23bp)
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA797460.3101317844244589No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA793050.3084167376894354No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG763160.2967925320409426No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG725270.28205713050125064Illumina Small RNA Adapter 2 (100% over 21bp)
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA722490.28097598992905887RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA718020.27923760922485136No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC697850.2713935065841655RNA PCR Primer, Index 1 (100% over 50bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA694450.27007124833040586No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA693050.26952678904944605No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA651900.25352357518409047No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA630660.2452633500929567RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG603810.2348213988831196No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG600730.2336235884650079No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG578000.22478390313913832Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG574600.22346164488537867No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA574430.2233955319726907No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA570460.2218516010116831RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC565830.22005099638965162No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA561390.21832428267003612RNA PCR Primer, Index 1 (100% over 23bp)
ATTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCC550580.21412027922205323No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA528950.20570838333122354RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG522930.2033672084230962No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC521350.20275274723458436No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGG488950.19015240387522783No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGG480380.18681953527678075Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC475840.1850539316085252RNA PCR Primer, Index 1 (95% over 24bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG474040.18435391253300543No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA467690.1818844007943661No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAGG460610.17913099243065483Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC459830.17882765083126292No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGGT457850.17805762984819112No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA453350.17630758215939163No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCA451520.1755958960992798No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG450120.17505143681831997No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG443250.1723796973467527Illumina Small RNA Adapter 2 (100% over 21bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCC425150.16534061664291463No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA414940.16136995288677172No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAA399210.1552525639657014No Hit
AATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAA390990.15205581018749428RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC353420.13744485648345028No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA349510.1359242594916267No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG340300.13234249522188368Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA323650.12586731877332547No Hit
ACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAG311900.1212977498081267No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG309100.12020883124620702Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCTGGAATTCTCGGGTGCCAAGGAA307220.11947770021177521RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA300400.11682540571452794No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA291470.11335253330097689RNA PCR Primer, Index 1 (100% over 23bp)
CTCGACTGCATACTTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG290730.11306474768104098No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC287500.11180860233996931RNA PCR Primer, Index 1 (100% over 26bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAG287240.11170748847350534No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC280790.10919908678622603No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC274870.10689680182673868No Hit
CTCGACTGCATCATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG271460.10557065457811503No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG264360.10280946822467578Illumina Small RNA Adapter 2 (100% over 21bp)
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG259760.10102053058723627No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAA259440.10089608275158832No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTGTGGAATTCTCGGGTG258980.10071718898784436No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAA257380.10009494980960454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)