FastQCFastQC Report
Fri 7 Jul 2023
SRR6255571.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255571.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75464180
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG17706852.346391360775404No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC16900952.2395989726516605RNA PCR Primer, Index 1 (100% over 24bp)
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC11507181.524853248256325No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT11042571.4632862902638046No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG9846211.3047527979499678No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC7988681.0586055529921614RNA PCR Primer, Index 1 (100% over 27bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA7391670.9794938472795967No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC5479290.726078253285201No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA5436400.720394762124229No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT5174440.6856816041729997RNA PCR Primer, Index 1 (100% over 25bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC5008080.6636367081706844No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTGC4964710.6578896106735672No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG4612530.6112211117910511No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA4247640.5628683701326908No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC3845050.5095198808229282No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG3831450.5077177012988149No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3704620.4909110521044554RNA PCR Primer, Index 1 (100% over 30bp)
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC3688370.4887577125995406RNA PCR Primer, Index 1 (100% over 26bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAA3203550.4245126628289077No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA3087330.40911197868975724No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA2878560.3814471978626151RNA PCR Primer, Index 1 (100% over 23bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2697160.357409303327751RNA PCR Primer, Index 1 (100% over 23bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT2680020.35513802707456704No Hit
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA2360680.31282126168998325No Hit
AACTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCA2328000.30849073030409924No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCTGGAATTCTCGGGTGCCAAG2300200.3048068633356912No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA2176840.28846003494638117No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCTGGAATTCTCGGGTGCCAAGG2164330.28680229481059755Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2060670.2730659764672458No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA2048500.271453290819565RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2021530.267879409807408RNA PCR Primer, Index 1 (100% over 50bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCAA1988590.26351442498944533No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA1941740.2573061815552756No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGTGGAATTCTCGGGTGCCAAGGA1896260.2512794812055203RNA PCR Primer, Index 1 (100% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAAG1816450.24070360268938193No Hit
GCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAAG1783090.23628296232729223No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC1729080.229125924378957RNA PCR Primer, Index 1 (95% over 24bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1726890.22883572047029463Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1706430.22612450039210655No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG1702450.2255970978549028No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1683580.22309657376519562No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1681360.22280239446052416RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA1664130.220519192019313RNA PCR Primer, Index 1 (100% over 22bp)
ATTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCC1619700.21463163052987524No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC1590680.2107860974570982No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA1571830.20828822363139704RNA PCR Primer, Index 1 (100% over 22bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG1539030.20394179066147677No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGG1473390.19524362419362404No Hit
AAACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGG1405740.18627910619316343Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG1405550.18625392868510596No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCAA1388950.18405420956008534No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCC1374980.18220300015186014No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCTGGAATTCTCGGGT1369480.18147417755019665No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA1355360.17960309116192608No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG1334040.1767779097314779No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAGG1306520.17313114645915453Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCCA1298790.17210681942081663No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAGG1275840.16906564147387543Illumina Small RNA Adapter 2 (100% over 21bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGACTGGAATTCTCGGGTGCC1253040.1660443405069796No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1216920.16125796371205517No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAA1162190.15400551625950218No Hit
AATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCAAGGAA1093260.14487138136265443RNA PCR Primer, Index 1 (100% over 23bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1046820.13871746834060875No Hit
CGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCAA1025450.13588566125014545No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAGG1024640.13577832555790045Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCCA976480.12939648983133456No Hit
CTCGACTGCATACTTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG967940.12826482710075165No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCTGGAATTCTCGGGTGCCAAGGAA922930.12230040795513845RNA PCR Primer, Index 1 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAGG892380.11825212968589865Illumina Small RNA Adapter 2 (100% over 21bp)
ACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAAG891250.11810238976955689No Hit
CTCGACTGCATCATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG885450.1173338132078027No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA882440.11693494847489232No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC862470.11428865986485243RNA PCR Primer, Index 1 (100% over 26bp)
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC857930.11368704993547933No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCTGGAATTCTCGGGTGCCAAGGAA849130.11252093377281777RNA PCR Primer, Index 1 (100% over 23bp)
AAACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAG840500.11137734485420765No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC816780.10823413174303358No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCCAA764410.10129441544319438No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGTGGAATTCTCGGGTGCCAAG764260.101274538463149No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG760960.10083724490215092Illumina Small RNA Adapter 2 (100% over 21bp)
AACTCGACTGCATAATTTGTGGTAGTGGGGGTGGAATTCTCGGGTGCCAA760470.10077231343400272No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)