FastQCFastQC Report
Fri 7 Jul 2023
SRR6255572_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255572_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39670271
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length60
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC9803322.4712006630859666RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC8164792.0581634040261534RNA PCR Primer, Index 1 (100% over 27bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG7246321.8266373829409939No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC4629551.16700740461289RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT3813880.9613949952598004RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3581340.902776792223073RNA PCR Primer, Index 1 (100% over 30bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3431920.8651113071549221No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA3427500.8639971226816171RNA PCR Primer, Index 1 (100% over 23bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG3271570.8246906102557252Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2876600.7251273882147162RNA PCR Primer, Index 1 (100% over 23bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT2679630.6754755973308072No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC2475350.6239811167410477RNA PCR Primer, Index 1 (95% over 24bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA2452140.618130387866521RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT2263320.5705330321539774No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA2131970.5374225953737498No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC2088440.5264496428572418No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT2073620.5227138478585135No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC1834420.4624168057737745RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA1757230.44295890996055964No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1418110.3574742405969447RNA PCR Primer, Index 1 (100% over 23bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA1358090.3423445229300299RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1290200.3252309519135879No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1249660.3150117124231392No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA1237410.3119237577177126No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG1217390.3068771574562725Illumina Small RNA Adapter 2 (100% over 21bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAAG1091130.27504979736589147No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG1090010.27476747007853813No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT1087560.2741498791374528RNA PCR Primer, Index 1 (100% over 25bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA1071460.2700914243817492No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA998650.2517376299244338No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC974450.24563734389412162No Hit
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA972660.2451861243902266RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG967210.2438122996437307No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC946360.23855647469612698No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC931130.23471732774399248No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA921830.2323730029472196No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC904330.2279616390823244No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG903190.22767427023626835No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCACGGAACT881710.22225963618952843RNA PCR Primer, Index 1 (96% over 25bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT854190.2153224514145618RNA PCR Primer, Index 1 (100% over 25bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC825850.20817856273278296No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC823470.20757861724715718No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG817690.2061216067820661No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA804640.20283198972852998No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA800480.20178334551836058RNA PCR Primer, Index 1 (100% over 22bp)
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG776180.19565785169453467RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG768780.19379247497452184No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA751080.18933069552259926No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG749930.18904080589719188No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG732330.184604234238783No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG728450.18362617185045194No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG718040.18100204054567715Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG699290.17627557926186085Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA690480.17405477265330505No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA687650.1733413920968677No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA672570.16954005683500373RNA PCR Primer, Index 1 (100% over 28bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG649180.16364395393215236No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA624100.15732183931891972No Hit
CCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC613500.1546498132064689RNA PCR Primer, Index 1 (100% over 26bp)
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG612620.15442798462354845No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG610950.15400701447186987No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA569410.14353569704628436No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA568880.14340209574066182No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA564730.14235597230984381No Hit
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA560120.14119389302886284No Hit
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGG553020.13940413968939108No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA549180.1384361604184655RNA PCR Primer, Index 1 (100% over 22bp)
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC546550.13777319544905556RNA PCR Primer, Index 1 (100% over 24bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCTGGAATTCTCGGGTGCCAAGG542210.13667917721056153Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA538380.13571371871898733No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC526730.13277701077464282RNA PCR Primer, Index 2 (100% over 50bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAAC513320.1293966456644574RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA507970.12804802871147516RNA PCR Primer, Index 1 (100% over 28bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG503620.1269514896936298No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG502690.1267170572139525No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC494290.12459960255880279No Hit
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA486180.12255525050484277No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC482740.12168810240797195No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA479660.1209117023677504RNA PCR Primer, Index 1 (100% over 22bp)
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA470910.1187060204353028No Hit
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT469730.1184085684718413RNA PCR Primer, Index 1 (100% over 25bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC468380.11806826325940652No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC462540.1165961281182072No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT457920.11543152805787488No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG457380.11529540597290096No Hit
GCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGCC443190.11171842007330879No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA443140.11170581617655195No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA432730.10908168487177715RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC431500.1087716290115588No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG430400.10849434328290826Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA418020.10537361844591382No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG411160.1036443638108749No Hit
GCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAAC409910.10332926639195382RNA PCR Primer, Index 1 (100% over 24bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC408930.10308223001551968No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC406300.10241926504610972No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)