FastQCFastQC Report
Mon 10 Jul 2023
SRR6255574.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255574.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96285407
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC23931372.4854617896562456RNA PCR Primer, Index 1 (100% over 24bp)
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC18562131.9278238082329548RNA PCR Primer, Index 1 (100% over 27bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG17108441.7768466201737092No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC10796351.1212862194164066RNA PCR Primer, Index 1 (100% over 26bp)
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT9338130.9698385550782374RNA PCR Primer, Index 1 (100% over 25bp)
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT8816480.9156610824732766RNA PCR Primer, Index 1 (100% over 30bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC8065860.8377032669135417No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG7541240.7832173363508761Illumina Small RNA Adapter 2 (100% over 21bp)
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA7340380.7623564389149853RNA PCR Primer, Index 1 (100% over 23bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA6886350.7152018373874661RNA PCR Primer, Index 1 (100% over 23bp)
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT6016860.6248984334666623No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA5717750.5938334975309395RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT5365350.5572339741992263No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC5040210.5234656171729117No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA4901960.5091072627443949No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT4862760.5050360331342838No Hit
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC4110210.4268777718309899RNA PCR Primer, Index 1 (100% over 26bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA4047200.4203336856643292No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA3749380.3894027264173064RNA PCR Primer, Index 1 (100% over 23bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA3327780.3456162365289685RNA PCR Primer, Index 1 (100% over 23bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA3147080.3268491143211349No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC3137780.32588323586771567No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG2990500.31058704461829817Illumina Small RNA Adapter 2 (100% over 21bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAA2939090.305247710070956No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT2709290.2813811650606618RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA2665170.2767989545913225RNA PCR Primer, Index 1 (100% over 22bp)
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTTGGAATTCTCGGGTGCCAAG2617860.2718854374266705No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA2616200.27171303331563007No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCCAGGAAC2593250.26932949455154714RNA PCR Primer, Index 1 (95% over 24bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCTGGAATTCTCGGGTG2575760.2675130199117297No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG2312560.24017762110098367No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA2310690.23998340683131764No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC2249480.2336262648814477No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2220390.2306050386223117No Hit
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT2208380.22935770526472404RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG2207580.22927461894615037No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCAA2207350.22925073162956044No Hit
TGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTCCAG2185250.22695547207896208RNA PCR Primer, Index 1 (100% over 29bp)
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC2172370.22561778234992555No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC2132240.22144996489447252No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA2004490.2081821183972354RNA PCR Primer, Index 1 (100% over 22bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1983040.2059543664804782No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA1918470.1992482619925987No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC1877350.19497762521791076No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1866470.19384765128530848No Hit
TGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACTCCA1812110.18820193593822582RNA PCR Primer, Index 1 (100% over 28bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTGGAATTCTCGGGTGCCAAG1788490.18574881238233742No Hit
CGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCCA1781530.1850259614107463No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG1774350.18428026170154735No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1729780.1796513151780103No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1691780.17570471504575974Illumina Small RNA Adapter 2 (100% over 21bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG1664020.1728216197912525No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG1647540.17111004162863433No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCGATGTATCTCGTATGC1643910.1707330374581062RNA PCR Primer, Index 2 (100% over 50bp)
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCTGGAATTCTCGGGTGCCA1619110.1681573615823216No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA1615270.16775854725316786No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1594810.16563361465564558Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG1579750.16406951470849573No Hit
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG1509660.15679011462245782No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG1457350.15135730796672023No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG1432090.14873385745775577No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1408100.14624230647952705No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA1393520.14472805832352145No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC1391140.14448087652576472RNA PCR Primer, Index 1 (100% over 24bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTG1379900.14331351374980425No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAAC1359770.14122285425869363RNA PCR Primer, Index 1 (100% over 24bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG1347830.13998279095398122No Hit
CCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGCCAAGGAACTC1334500.138598365170747RNA PCR Primer, Index 1 (100% over 26bp)
TCCCCGCGGGGCCCCGTCGTCCCCCGCGTCGTTGGAATTCTCGGGTGCCA1333720.13851735601013765No Hit
GGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAA1313500.13641734930818747No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA1304600.1354930140140551RNA PCR Primer, Index 1 (100% over 22bp)
CGGTGGGGGGCGGGGCGGACTGTGGAATTCTCGGGTGCCAAGGAACTCCA1292170.1342020603392163RNA PCR Primer, Index 1 (100% over 28bp)
TGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGG1261330.13099908275820032No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA1246180.12942563560021095No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCTGGAATTCTCGGGTGCCAAGG1233900.1281502606101047Illumina Small RNA Adapter 2 (100% over 21bp)
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT1225720.12730070300268867RNA PCR Primer, Index 1 (100% over 25bp)
TTGGCAATTTTTGACAGTCTCTACGGAGACTGTGGAATTCTCGGGTGCCA1212070.12588304269202497No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1208770.12554031162790846RNA PCR Primer, Index 1 (100% over 22bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1171290.12164771760273081No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCTGGAATTCTCGGGTGCC1168100.12131641090741818No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC1152800.11972738506469625No Hit
GCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAAC1151700.11961314137665742RNA PCR Primer, Index 1 (100% over 24bp)
GGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCA1140330.11843227707392875No Hit
GGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCA1093120.1135291456990985RNA PCR Primer, Index 1 (100% over 28bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC1089850.11318953037192854No Hit
CCGTCCCCGCTCGGCGGGGTCCCCGCGTCGTCCTGGAATTCTCGGGTGCC1089790.1131832988980355No Hit
GCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGCC1075360.11168462942676245No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG1039270.10793639788010659Illumina Small RNA Adapter 2 (100% over 21bp)
GCGTTGGTGGTATAGTGGTGAGCATAGCTGGAATTCTCGGGTGCCAAGGA1037340.10773595213654755RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGT1037310.10773283639960105No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGGA1024850.10643876698781571RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1012470.10515300620788776No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCC1008660.10475730761568054No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG1008090.10469810861369677Illumina Small RNA Adapter 2 (100% over 21bp)
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC1003560.10422763233477322No Hit
CGCCTGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGG1002080.10407392264541189Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC986370.10244231506442093No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA980820.10186590372931591No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA974170.10117524870617205No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG971270.10087406080134241No Hit
CGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCA966960.10042643326002662No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)