FastQCFastQC Report
Fri 7 Jul 2023
SRR6255576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37708039
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG6032921.5999028748219974No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA4431211.1751366863707762No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA3357720.8904520333184125No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC3319340.8802738323252504RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA3318090.8799423380250561No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC3193760.8469705889505418RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC3152010.835898679324056No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC2954050.7834005899909036RNA PCR Primer, Index 1 (100% over 24bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA2499830.6629435171635417RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA2300170.6099945955821251RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA2255310.5980979281367562RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG2251460.5970769256921581No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA1975510.52389624398129No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG1945150.5158449104181737Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG1897190.5031261371083232No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG1788510.47430469667224007Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT1643640.435885833256935No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG1575000.4176828182446719Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA1536440.40745688207228176No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA1522470.4037521017733115No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA1438160.3813934742138142RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC1433710.3802133545051229No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG1254880.3327884539421422No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT1253410.3323986166451138RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC1234070.3272697368325094RNA PCR Primer, Index 1 (100% over 26bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT1223650.3245064003460907RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC1210470.3210111244448432No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC1193900.31661683600146906No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT1141110.3026171687156683RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC1085190.2877874397021813No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG1071480.28415161021765145No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA1037630.27517474456839297No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC1032510.27381694391479755RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC1023720.27148587599583207No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA1005850.266746833480256No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC991340.26289884764360194No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA987380.26184867370058673RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA963470.2555078507264724RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA907080.2405534798561124RNA PCR Primer, Index 1 (100% over 22bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC879430.23322082593581706RNA PCR Primer, Index 3 (100% over 50bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA860680.22824841143290428No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT854550.226622763384752No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA854250.2265432047527054No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT826880.21928480555565352No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG826780.219258286011638No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT807180.21406045538459317RNA PCR Primer, Index 1 (100% over 25bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA779180.20663498306024347No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC772880.2049642517872648No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG770140.20423761628123915No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG759790.2014928434756313No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA749700.1988170214844638RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG724040.19201210649007763No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG713550.189230206322848Illumina Small RNA Adapter 2 (100% over 21bp)
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG712890.1890551773323455No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG694040.1840562432854172No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCCA685570.1818100379073014No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG674460.1788637165671755No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC649600.17227095792491356No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG633120.16790053707115346No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG622740.16514780840234095Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG619340.16424614390581274No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG586700.15559016473914222Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC584070.15489270073153366No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA583280.15468319633381095No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAGG580450.1539326932381713Illumina Small RNA Adapter 2 (100% over 21bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG580060.1538292670165107Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC571210.1514822873711359No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA566200.1501536582159576RNA PCR Primer, Index 1 (100% over 22bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG559570.14839541244772766No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG548850.14555251732926233Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA532340.14117414061229755No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG527770.1399621974507876No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA524790.13917191503912468RNA PCR Primer, Index 1 (100% over 22bp)
CGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAAG517430.13722007659958133No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA509900.13522315493521156No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA500340.13268788652732644No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG497980.13206202528855984Illumina Small RNA Adapter 2 (100% over 21bp)
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA496810.13175174662357808No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA494840.13122931160647203No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA489930.12992720199530927No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAA487520.1292880809845349No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAG485610.12878155769383817No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG481850.12778442283885408Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG457300.12127387478304029No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG456170.12097420393566474No Hit
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT451800.11981529986218589RNA PCR Primer, Index 1 (100% over 25bp)
GCGTTGGTGGTATAGTGGTGAGCATAGTGGAATTCTCGGGTGCCAAGGAA451740.11979938813577656RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG451060.11961905523647093No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG449740.11926899725546589No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG442740.11741262917437845Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA440890.11692201761009105RNA PCR Primer, Index 1 (100% over 23bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA438170.1162006860128685No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT437850.11611582347201879No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCA434680.11527515392672635No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGTGGAATTCTCGGGTGCCA422980.11217236727690878No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG420400.11148816304130799Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGC419430.1112309234643573No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA418530.11099224756821748No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC414990.11005345571006755No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTTGGAATTCTCGGGTGCCAAG412600.10941963860809628No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA411510.10913057557832694No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC411360.10909079626230365No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGC407510.10806979381770557No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG405920.10764813306785855No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC405170.10744923648774204No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAATGGAATTCTCGGGTGCCAA403540.10701696792028884No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA398280.1056220399050717RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG395610.10491396807985691No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC390100.10345274120460096No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA384690.10201803387336053No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)