FastQCFastQC Report
Wed 5 Jul 2023
SRR6255577.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6255577.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110629054
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTG17968351.6241981062226203No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCA13226751.1955946039274639No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGTGGAATTCTCGGGTGCCAA10037880.9073457321618242No Hit
CGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCC9928820.8974875623540992RNA PCR Primer, Index 1 (100% over 27bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAA9922090.8968792230655791No Hit
CCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTC9498400.858580965539125RNA PCR Primer, Index 1 (100% over 26bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGC9402820.8499412821517935No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAAC8616200.7788369952074253RNA PCR Primer, Index 1 (100% over 24bp)
GGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAA7428970.6715207019667726RNA PCR Primer, Index 1 (100% over 23bp)
GGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGA6828280.617223030760075RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGTGGAATTCTCGGGTGCCAAGGA6710340.6065621785033071RNA PCR Primer, Index 1 (100% over 22bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTG6707300.6062873863135447No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGTGGAATTCTCGGGTGCCAA5841220.528000537724927No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGAATTCTCGGGTGCCAAGG5789220.5233001450053075Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGTGGAATTCTCGGGTGCCAAG5679490.5133814124452334No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGG5317980.4807037399054321Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGT4889550.441977023504151No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAGG4716480.4263328510429096Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGTGGAATTCTCGGGTGCCAA4587290.4146550868996855No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGATGGAATTCTCGGGTGCCAA4496570.4064547094473031No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTGGAATTCTCGGGTGCCAAGGAA4276180.38653317961120776RNA PCR Primer, Index 1 (100% over 23bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC4251020.38425891267225337No Hit
CGCGGGAGACCGGGGTTCGATTCCCCGACGTGGAATTCTCGGGTGCCAAG3750630.3390275758843604No Hit
CGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAACT3730270.33718719135029396RNA PCR Primer, Index 1 (100% over 25bp)
CGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACTC3686360.33321807126724595RNA PCR Primer, Index 1 (100% over 26bp)
CCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGAACT3614700.32674056853093947RNA PCR Primer, Index 1 (100% over 25bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGTGGAATTCTCGGGTGCC3608450.32617561748290824No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGTGGAATTCTCGGGTGCC3564100.32216672484607883No Hit
TGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGT3423750.3094801841114903RNA PCR Primer, Index 1 (100% over 30bp)
CGCGGGAGACCGGGGTTCGATTCCCCGACGGGGATGGAATTCTCGGGTGC3218150.290895554435456No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTGGAATTCTCGGG3177210.28719489909043244No Hit
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGGTGGAATTCTCGGGTGCCA3077960.278223476447697No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCC3065430.2770908625866041No Hit
CCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGGAAC3027350.2736487288411596RNA PCR Primer, Index 1 (100% over 24bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTATGGTGGAATTCTCGGGTGCCA2986930.2699950774233322No Hit
CGCGGGAGGCCCGGGTTCGATTCCCGGCCAATGCTGGAATTCTCGGGTGC2941520.26589036908875674No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA2914240.2634244707543102RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTTGGAATTCTCGGGTGCCAAGGAA2852060.2578038857676574RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGGCGCAATGAAGGTGAAGGGCCCCTGGAATTCTCGGGTGCCAAGGA2629910.2377232657164365RNA PCR Primer, Index 1 (100% over 22bp)
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTTGGAATTCTCGGGTGCCA2584100.23358240051478701No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2534490.2290980450759346RNA PCR Primer, Index 3 (100% over 50bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGGAATTCTCGGGTGCCA2531360.22881511759108053No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCTGGAATTCTCGGGT2527910.22850326461256731No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATGGAATTCTCGGGTGCCAAG2504590.22639531926215334No Hit
CGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTCTCGGGT2460320.22239365799873873No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGCCAAGGAACT2401970.21711927501431946RNA PCR Primer, Index 1 (100% over 25bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGTGGAATTCTCGGGTGCCAA2312020.20898849953105447No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGCC2295100.20745906405382442No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAG2287720.20679196985630915No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTATTGGAATTCTCGGGTGCCAAG2265470.2047807441253181No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGGA2222320.2008803220897107RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG2149290.19427898208367578No Hit
CGCAGAAGGTCCTGGGTTCGAGCCCCAGTGTGGAATTCTCGGGTGCCAAG2115810.19125265230958227No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTG2106780.19043641103538678No Hit
AAGCGTGTACTCCGAAGAGGATCCAAAGATGGAATTCTCGGGTGCCAAGG2050660.18536360258490503Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGTGGAATTCTCGGGTGCCA2024780.18302425328521746No Hit
CGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTG2004150.1811594628658761No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGAGTGGAATTCTCGGGTGCC1930210.1744758659872478No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAG1885000.17038923608620932No Hit
CGGGCGGGCGTCCCGCGCGTGTGGGGGGATGGAATTCTCGGGTGCCAAGG1867420.16880014177830718Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGATGGAATTCTCGGGTGCCAAG1786000.16144041148539515No Hit
GGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAGG1759620.15905586610186506Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCAAGGTCGCGGGTTCGATCCCCGTACGGTGGAATTCTCGGGTGCCAA1755440.15867802684094182No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGTGGAATTCTCGGGTGCC1741340.15740349727658343No Hit
GGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAAGG1726080.15602411279771045Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTGGAATTCTCGGGTGCC1703200.15395594000107785No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTTGGAATTCTCGGGTGCCAAGG1663830.1503972003593197Illumina Small RNA Adapter 2 (100% over 21bp)
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTGGAATTCTCGGGTGCCAAGG1645790.14876652565428247Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGGGCCCGAGGTGGGATCCCGAGGCCTCTGGAATTCTCGGGTGCCAAG1621670.14658626656972046No Hit
AACGATGCCGACTGGCGATGCGGCGGCGTGGAATTCTCGGGTGCCAAGGA1618860.14633226457852563RNA PCR Primer, Index 1 (100% over 22bp)
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCTGGAATTCTCGGGTGCCA1605310.145107450706394No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAG1563880.1413625031992048No Hit
CCCCGCCCCTTGCCTCTCGGCGCCCCCTTGGAATTCTCGGGTGCCAAGGA1556940.14073518155547096RNA PCR Primer, Index 1 (100% over 22bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGTGGAATTCTCGGGTGCCA1527210.13804782241019614No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAAG1503120.1358702750906647No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGGAATTCTCGGGTGCCAA1497210.1353360573796464No Hit
AGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTTGGAATTCTCGGGTGCCA1484560.13419259645843123No Hit
TGCGAGAGGTCCCGGGTTCAAATCCCGGACGATGGAATTCTCGGGTGCCA1456920.13169415694361808No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCCAA1455940.1316055726192868No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGTGGAATTCTCGGGTGCCAA1454410.13146727260272875No Hit
CGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCAAGG1449720.13104333333628615Illumina Small RNA Adapter 2 (100% over 21bp)
ACGCGGGAGACCGGGGTTCGATTCCCCGACTGGAATTCTCGGGTGCCAAG1443190.13045307248130317No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCATGGAATTCTCGGGTGCCAAGG1434520.12966937238747428Illumina Small RNA Adapter 2 (100% over 21bp)
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCTGGAATTCTCGGGTG1362010.12311503630863553No Hit
GGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGGAATTCTCGGGTGCCAAG1350550.12207914206696553No Hit
CGCAGAAGGTCCTGGGTTCGTGCCCCAGTGTGGAATTCTCGGGTGCCAAG1336610.12081907524943675No Hit
GCGTTGGTGGTATAGTGGTGAGCATAGTGGAATTCTCGGGTGCCAAGGAA1331840.12038790460957934RNA PCR Primer, Index 1 (100% over 23bp)
CCCGGTGGGGGGCGGGGCGGACTGTTGGAATTCTCGGGTGCCAAGGAACT1327280.11997571632493577RNA PCR Primer, Index 1 (100% over 25bp)
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCTGGAATTCTCGGGTGCCAA1316370.11898953777549251No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGATGGAATTCTCGGGTGCCAAGG1314640.11883315932539748Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCCGCCCCTTGCCTCTCGGCGCCCCTGGAATTCTCGGGTGCCAAGGAA1304770.11794098863034659RNA PCR Primer, Index 1 (100% over 23bp)
TCCGTAGTGTAGTGGTTATCACGTTCGCCTTGGAATTCTCGGGTGCCAAG1299650.11747818073179943No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCTGGAATTCTCGGGT1298930.11741309837106624No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGTGGAATTCTCGGGTGCCA1290550.11665561200586601No Hit
CGCGGGAGACCGGGGTTCAATTCCCCGACGGGTGGAATTCTCGGGTGCCA1251690.11314297236962724No Hit
GCGGGAGACCGGGGTTCGATTCCCCGACGGGGAGTGGAATTCTCGGGTGC1249600.1129540527391656No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGTGGAATTCTCGGGTGCCAAGG1240300.1121134055796952Illumina Small RNA Adapter 2 (100% over 21bp)
CGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGTGGAATTCTCGGGTGCCA1236480.11176810749913851No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGGAATTCTCGGGTGCCAA1229390.11112722703025192No Hit
CCCAGGCGGCCCGGGTTCGACTCCCGGTGTTGGAATTCTCGGGTGCCAAG1227920.11099435054375498No Hit
ACGCGGGAGACCGGGGTTCGATTCCCCGACGGGTGGAATTCTCGGGTGCC1226650.11087955249079505No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGTGGAATTCTCGGGTGCCAAG1223010.11055052500042169No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCTGGAATTCTCGGGTGC1218860.11017539750452895No Hit
CGCGGGAGGCCCGGGTTCGTTTCCCGGCCAATGCTGGAATTCTCGGGTGC1213550.10969541509412166No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGTGGAATTCTCGGGTGCC1211150.10947847389167768No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAATGGAATTCTCGGGTGCCAA1211020.10946672290987863No Hit
CGCCGCGGCCCGGGTTCGATTCCCGGTCTGGAATTCTCGGGTGCCAAGGA1202190.10866856006922015RNA PCR Primer, Index 1 (100% over 22bp)
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGTGGAATTCTCGGGTGCCAAG1177200.1064096597987722No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGGAATTCTCGGGTGC1168500.10562324793991279No Hit
GGCATTAACGCGAACTCGGCCTACAATAGTGATGGAATTCTCGGGTGCCA1139060.10296210252326662No Hit
CACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGGAATTCTCGGGTGCC1107990.10015361787329394No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)