FastQCFastQC Report
Tue 4 Jul 2023
SRR6286686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31815773
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCGTAGATCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC15648884.9185917940764785Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACT11711033.680888092833702Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGGATATTTAAGTGAGATGAGATCGGAAGAGCACACGTCTGAACTCC7787902.447811027567993Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC6735412.117003412112602Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATG5049801.5872001601218364TruSeq Adapter, Index 16 (97% over 49bp)
AGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC5007241.5738231474055338Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AAAGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA4603691.446983544922828Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGGCGTAGATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC4360601.3705780463042656TruSeq Adapter, Index 16 (100% over 35bp)
CGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACTCCA2850290.8958732512958274Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACGCTAGGTACTGCAAACTGAGATCGGAAGAGCACACGTCTGAACTCCAG2708920.8514393159644431Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC2059940.6474587306113858Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1750100.5500730722462723Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC1473360.4630910586393736TruSeq Adapter, Index 16 (100% over 39bp)
AGAGGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA1331760.41858483212084774TruSeq Adapter, Index 16 (97% over 39bp)
AGTGCGTAGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGT1162560.365403663145321No Hit
TGAGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTGAAC1051740.3305718833234069Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTGAACTC1013210.31846153792963006Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCCGGCAGTCAGACTGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1003920.3155416025881251Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCTGTGGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT997070.3133885824493404TruSeq Adapter, Index 16 (100% over 37bp)
GCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACTCC911530.2865025470228242Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTGAATTCAGAAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC896670.28183190771445343Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA878630.27616176416647176Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGAGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC840080.26404513258250867Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAAGGATGTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT822210.25842842165111TruSeq Adapter, Index 16 (100% over 37bp)
AAGGATGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC814870.25612138985276267TruSeq Adapter, Index 16 (100% over 39bp)
TGAGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA798790.25106729294303176Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTGAGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTG759810.23881550827006465Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAACT755960.23760541665921492Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGTTAACTGTATGGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTC746970.2347797741705034Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCGTAGAGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC731720.22998655415350117TruSeq Adapter, Index 16 (100% over 35bp)
AAGCTCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC722520.22709490666783422TruSeq Adapter, Index 16 (100% over 39bp)
AGGTAAAGAGGACAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC711830.22373493801329294Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGTGCGTAGGCGGATATTTAAGTGAGATGAGATCGGAAGAGCACACGTCT692760.21774105567072033Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAAC682360.21447223677387942Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCGTAGAGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG647820.20361598632225594TruSeq Adapter, Index 16 (100% over 36bp)
AGGATGTGTGATTTCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC647560.20353426584983492Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGACGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT626560.19693376615429084TruSeq Adapter, Index 16 (97% over 41bp)
ACGCTAGGTACTGCAAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC622600.19568910049741678Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGGATGTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC613910.19295775086149877TruSeq Adapter, Index 16 (100% over 38bp)
ATCGCTGCTGTAGAAATACAGGAGATCGGAAGAGCACACGTCTGAACTCC604960.19014468075315977Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA598360.18807023799170305TruSeq Adapter, Index 16 (97% over 39bp)
AACGCTAGGTACTGCAAACTGAGATCGGAAGAGCACACGTCTGAACTCCA598050.18797280204381644Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT596280.18741647421233487TruSeq Adapter, Index 16 (97% over 41bp)
TAGCCGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC577790.18160489138516295TruSeq Adapter, Index 16 (100% over 39bp)
AGGGCGTAGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCG559590.1758844583156914TruSeq Adapter, Index 16 (100% over 36bp)
AGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC525280.1651004990512096Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT493120.15499230523174778TruSeq Adapter, Index 16 (100% over 37bp)
TTGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT491410.1544548359708249TruSeq Adapter, Index 16 (97% over 41bp)
AGGATGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA481860.1514531801568989TruSeq Adapter, Index 16 (97% over 39bp)
AGGTAAAGAGGACAGGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA473290.14875954766209828Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCAT467320.1468831198915079TruSeq Adapter, Index 16 (97% over 41bp)
TCTTTGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT462510.14537129115171898TruSeq Adapter, Index 16 (100% over 37bp)
TCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC457410.1437683126542297Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTTTCGTGTGCCGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA451210.14181959369649763Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGACGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA446600.14037062685857105TruSeq Adapter, Index 16 (97% over 39bp)
AGGACTGCACTCGGAGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC444050.13956913760982642Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AAAGGATGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTC442030.13893423240101693TruSeq Adapter, Index 16 (100% over 38bp)
TGTGAGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTGA440120.13833390123823172Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTTTCGTGTGCCGTGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC432510.1359420058723703Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGAGCTGTGGATAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA423070.13297492410446857Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGTGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAAC414510.13028443470476106Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AATGGATGTATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGT402260.12643414321569368TruSeq Adapter, Index 16 (100% over 37bp)
TGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAACTCC384680.12090858204199532Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGAGCTGTGGATAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC382340.12017309779020614Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGAGTTAGGATTTGCTAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC381560.11992793637294306Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTGATGGACAATCGGTTGAAATTCCGATACCGCATAAAAGCGAGATCG363450.11423579115930955No Hit
AGCTCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCA362400.11390576617453237TruSeq Adapter, Index 16 (97% over 39bp)
AGGATGTGTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCC360560.11332743667739897TruSeq Adapter, Index 16 (100% over 39bp)
AGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATC358980.11283082765268661TruSeq Adapter, Index 16 (97% over 42bp)
AGGGCGTAGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACC349310.1097914546976432Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGGTACTGCAGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCC346120.10878880736293914TruSeq Adapter, Index 16 (100% over 35bp)
TGGGATTTGATTGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA343570.10798731811419449Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AAGGTCAGAACTTAAGCCCCAGAGATCGGAAGAGCACACGTCTGAACTCC342700.10771386884109338Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTGGGAATTGCAGATGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC330050.10373785354830134Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACTCC319430.1003998865594119Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)