FastQCFastQC Report
Thu 13 Jul 2023
SRR6286687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31226831
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGCGTAGATCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA29319419.389172407536327Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGGCGTAGATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAT11988803.839262459901871TruSeq Adapter, Index 1 (100% over 35bp)
AGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACT9721523.1131945473429563Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGCGGATATTTAAGTGAGATGAGATCGGAAGAGCACACGTCTGAACTCC8997642.8813810789830065Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATTCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC5424221.7370382540578644Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC4813241.5413795911599228Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA3599671.152749057373129Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2814010.9011513207984505Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG2811510.9003507272319756TruSeq Adapter, Index 15 (97% over 49bp)
AAGAACCTTACCTGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA2627610.8414590644820795Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACTCCA2587280.8285438890677059Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCTGTGGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT1799170.5761615707978821TruSeq Adapter, Index 15 (100% over 37bp)
ACGCTAGGTACTGCAAACTGAGATCGGAAGAGCACACGTCTGAACTCCAG1780080.5700482383242795Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCCGGCAGTCAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1697210.5435101627827684Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTAAAGAGGACAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1233840.39512174642377257Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TAGCCGTAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA1141490.36554782007818853TruSeq Adapter, Index 15 (100% over 39bp)
ATTCCGGCAGTCAGACTGCGAGATCGGAAGAGCACACGTCTGAACTCCAG1102810.35316103641768837Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACTGAATTCAGAAGCCTGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1037660.3322975680753516Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGTGCGTAGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGT1011160.3238112762707173No Hit
AGGGCGTAGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG973850.31186321788464544TruSeq Adapter, Index 15 (100% over 36bp)
AGTGCGTAGGCGGATATTTAAGTGAGATGAGATCGGAAGAGCACACGTCT823450.26369950892551347Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGGCGTAGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA787050.2520428665976384Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCTTTGGGGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT718530.23010019812769347TruSeq Adapter, Index 15 (100% over 37bp)
GCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAACTCC691690.2215050255980186Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGCTGCTGTAGAAATACAGGAGATCGGAAGAGCACACGTCTGAACTCC622890.1994726906486284Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TAGGCGGATATTTAAGTGAGATGTGAGATCGGAAGAGCACACGTCTGAAC586470.1878096435722216Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGT571200.1829196180681927TruSeq Adapter, Index 15 (100% over 37bp)
AAAGGATGTGTGATTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA514390.1647269298636163Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTGATGGACAATCGGTTGAAATTCCGATACCGCATAAAAGCGAGATCG507130.16240200614657313No Hit
AGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA491420.15737107617484464Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACGCTAGGTACTGCAAACAGATCGGAAGAGCACACGTCTGAACTCCAGTC458680.1468865028282889Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGTGTGATTTCTAAGACAACTAGGATGTTGGCTCAGATCGGAAGAGCAC436080.13964913698735554No Hit
AAGGTCAGAACTTAAGCCCCAGAGATCGGAAGAGCACACGTCTGAACTCC433020.1386692104619902Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA432500.13850268700016344TruSeq Adapter, Index 15 (100% over 39bp)
AGACAGTTAGAGGAATGGACGTAGATCGGAAGAGCACACGTCTGAACTCC431340.13813121158531905Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGGACTGGATATGGAAGACATGAGATCGGAAGAGCACACGTCTGAACTCC422390.1352650866173388Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACTCC419400.13430757671183477Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGTGTGATTTCTAAGACAACTAGGATGTTGGCTCAGATCGGAAGAGCACA419280.13426914822064398No Hit
TACAGGCTGCAGACGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACA417170.13359344725053915Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGACGAAGCAAGGTGCACGACAGATCGGAAGAGCACACGTCTGAACTCCA410620.1314958921063748Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTGAACTC408390.13078176264507915Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACGCTAGGTACTGCAAACTGAGATCGGAAGAGCACACGTCTGAACTCCA407750.13057681069206156Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACATGGAGACTGATTTAAAGGAGTAGATCGGAAGAGCACACGTCTGAACT404180.1294335630791354Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGAGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTGAAC401210.12848245792216315Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTCCA391360.12532811927025192Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGGCGGATATTTAAGTGAGATGAGATCGGAAGAGCACACGTCTGAACTC388620.1244506687213954Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATGTGAGGTCTGGCCCATAAAAGTGGCGAGATCGGAAGAGCACACGTCTG373560.1196278930769504Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATGAGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC362020.11593235317410211Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TGAGCTGTGGATAGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA361770.1158522938174546Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGAGCTGTGGATAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC353430.11318151367969423Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCGGCAGTCAGACTGCGAGATCGGAAGAGCACACGTCTGAACTCCAGT337970.10823064306461326Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AGTGCGTAGGCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATG332480.10647253959263431TruSeq Adapter, Index 15 (100% over 36bp)
ATGAGCTGTGGATAGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA328470.10518838751200851Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TGGAGACTGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAA327180.10477528123170744TruSeq Adapter, Index 15 (97% over 39bp)
AAGCTCTGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCA325510.10424048472930218TruSeq Adapter, Index 15 (100% over 39bp)
ATTCCGGTCTTCAGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCA318460.10198281087184288Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGAGACAGTTAGAGGAATGGACGTAGATCGGAAGAGCACACGTCTGAAC314120.10059298044044239Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)