FastQCFastQC Report
Tue 4 Jul 2023
SRR6286690_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286690_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95785654
Total Bases2.7 Gbp
Sequences flagged as poor quality0
Sequence length20-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC1378881014.395485570313065No Hit
TGATGAGGTGTTATGGGGAACCCG83413138.708311371972258No Hit
TGATGAGGTGTTATGGGGAATCCG43494944.540861620050117No Hit
TTCCTGGGGTTGCGGATAGGTC17142871.7897116409519946No Hit
ATGATGAGGTGTTATGGGGAACCCG16362811.7082735583764976No Hit
AACGATGAGTACTAGGTGTAGGAGG14749581.539852721577701No Hit
ACGATGAGTACTAGGTGTAGGAGG14496511.5134322724361207No Hit
TGTTCCTGGGGTTGCGGATAG12360191.2904009612963543No Hit
CCGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC9617591.0040741591637512No Hit
TGGAATTCTAACCGGGCTCCATGAAACTGGAG9610981.0033840767010893No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAT9109330.9510119333736553No Hit
TCACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC8921240.931375381119181No Hit
ATGATGAGGTGTTATGGGGAATCCG8280540.8644864501316659No Hit
CGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC7826990.817135935617248No Hit
AATGATGAGGTGTTATGGGGAACCCG7566050.7898938603060538No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAGG6948960.7254698078273809No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAG6523920.681095730682175No Hit
TTCCTGGGGTTGCGGATAGG6366060.664615183396879No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACTAC6160520.6431568552008843No Hit
AAGGTCAGAACTTAAGCCCCAG6081880.6349468574907887No Hit
ACATGGAGACTGATTTAAAGGAGT5744210.5996941880252756No Hit
TTCCGTGGAGGACCGAACCACGTTGGTG5641710.5889932118644824No Hit
GGCTCCGCCGGAGAAGGTAAAAGCCCTGTAAATGAAAAC5105150.5329764726563333No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACT4916900.5133232164390713No Hit
CGATGAGTACTAGGTGTAGGAGG4766350.49760583145363296No Hit
ACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTG4155770.4338614214608797No Hit
ATGAGTACTAGGTGTAGGAGG3839620.4008554349902962No Hit
TGATGAGGTGTTATGGGGAACCCA3573290.37305064493269524No Hit
GGTGAAATCAGGAGTTCGATTCTCCTAGGGAC3556390.3712862888632571No Hit
ACCGACCGCTGGTGAACCAG3316110.34620111274700904No Hit
TCAAAAACTGCCCCCAGTTCGGATTGCAGGC3235270.33776143554858434No Hit
AGGACTGCACTCGGAGATGCTC3178230.3318064728148121No Hit
TGATGAGGTGTTATGGGGAACCC3021360.3154292812992643No Hit
ACTGCAGTTTAGCACTGAATTTCGGTATTGTCTG2911840.30399541877116587No Hit
TGATGAGGTGTTATGGGGAACCCC2748050.2868957808650552No Hit
TGATGAGGTGTTATGGGGAACCCGCAAGGGGAAGTACCTGAT2728590.28486416139101584No Hit
GCGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC2408000.2513946399530769No Hit
ACAACGCTAGGTACTGCAAACTG2347320.24505966206588722No Hit
TGATGAGGTGTTATGGGGAACCCGC2340330.24432990769160484No Hit
ATGATGAGGTGTTATGGGGAACCCGC2330170.2432692060545935No Hit
AATTCTAACCGGGCTCCATGAAACTGGAG2317130.24190783308740577No Hit
AATGGATGTATGTCTAAGCATCGAGTCAGG2237110.2335537636982674No Hit
TTGTTCCTGGGGTTGCGGATAGGTC2183580.2279652441481477No Hit
TGATGAGGTGTTATGGGGAATCC2178840.22747038925056565No Hit
ATTCCGTGGAGGACCGAACCACGTTGGTG2173470.2269097624995075No Hit
TTGTTCCTGGGGTTGCGGATAG2152630.2247340713464252No Hit
TACTGCAGTTTAGCACTGAATTTCGGTATTGTCTG2093780.2185901450336185No Hit
AAAAAGGGTGATGAATATTCAGG1931840.20168364669723923No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGGC1860960.19428379118234138No Hit
TGATGAGGTGTTATGGGGAATCCA1857650.1939382279521733No Hit
TGAGCACTCTAGCAAGACTGCCGCGG1831670.19122592199454003No Hit
ATTCTAACCGGGCTCCATGAAACTGGAG1782330.1860748374699201No Hit
AATGATGAGGTGTTATGGGGAATCCG1779660.18579609009090234No Hit
ATGATGAGGTGTTATGGGGAATC1770830.18487424014456277No Hit
ACGCTAGGTACTGCAAACTG1680520.17544589714864817No Hit
AAGGACTGCACTCGGAGATGCTC1622030.1693395547521135No Hit
CGATGAGGTGTTATGGGGAACCCG1621150.1692476829567818No Hit
TTGAGCACTCTAGCAAGACTGCCGCGG1615750.16868392421270098No Hit
AAAAACTGCCCCCAGTTCGGATTGCAGGC1589040.1658954064248494No Hit
GGATGAGGTGTTATGGGGAACCCG1566720.163565203615982No Hit
TGTTCCTGGGGTTGCGGATAGGTC1565550.1634430558880978No Hit
CACGATGAGTACTAGGTGTAGGAGG1554800.16232075838830728No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGG1550610.16188332336280756No Hit
TGATGAGGTGTTATGGGGAATCCC1449050.15128048298339122No Hit
TGGAATTCTAACCGGGCTCCATGAAACTGG1429290.14921754357912512No Hit
AATGGATGTATGTCTAAGCATCGAGTCAG1402440.14641440982383438No Hit
TGTTCCTGGGGTTGCGGATA1320780.1378891248161233No Hit
NCGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC1311900.1369620548814126No Hit
AGACGAAGCAAGGTGCACGAC1309580.1367198474209927No Hit
ACCCTGATAGTACTGGAATTCTAACCGG1255080.13103006009647333No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAG1249880.13048718130587697No Hit
AAACGATGAGTACTAGGTGTAGGAGG1231640.12858292954809286No Hit
AGACAACGCTAGGTACTGCAAACTG1175090.12267912270035762No Hit
TTCCTGGGGTTGCGGATAGGTT1155900.12067569116352227No Hit
AGGTCAGAACTTAAGCCCCAG1155200.1206026113263266No Hit
TGATGAGGTGTTATGGGGAAT1105720.11543691083426752No Hit
AGGCTCTGTGGGAGAATAGGTC1059820.11064496150958054No Hit
AAAAACTGCCCCCAGTTCGGATTGCAGGCTG1051850.10981289536322422No Hit
TGGACCGTAACTGACGCTGAGGCACG1022130.10671013427543127No Hit
GATGAGTACTAGGTGTAGGAGG1021020.10659425053359242No Hit
AATGGATGTATGTCTAAGCATC1016990.10617351947088026No Hit
AGGTGATGGACAATCGGTTGAAATTCCGATACCGCATAAAAGCG1005990.10502512202923416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)