FastQCFastQC Report
Tue 4 Jul 2023
SRR6286691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74932501
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length20-50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC70251849.375349689716082No Hit
TGATGAGGTGTTATGGGGAACCCG63247938.44065380921958No Hit
TGATGAGGTGTTATGGGGAATCCG44787765.977080626202507No Hit
AACGATGAGTACTAGGTGTAGGAGG17750652.368885298516861No Hit
ACGATGAGTACTAGGTGTAGGAGG15764322.103802727737594No Hit
TGGAATTCTAACCGGGCTCCATGAAACTGGAG12595171.6808687594719411No Hit
ATGATGAGGTGTTATGGGGAACCCG12576721.6784065435104054No Hit
TTCCTGGGGTTGCGGATAGGTC12193691.6272898725214042No Hit
TGTTCCTGGGGTTGCGGATAG10495461.4006552377051982No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAGG9862901.3162379299204225No Hit
ATGATGAGGTGTTATGGGGAATCCG8224491.097586479864058No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAG7675091.0242671601205464No Hit
AATGATGAGGTGTTATGGGGAACCCG6533780.8719554149140172No Hit
TCAAAAACTGCCCCCAGTTCGGATTGCAGGC6477800.8644846913624302No Hit
TTCCTGGGGTTGCGGATAGG6372350.8504120261513759No Hit
AAGGTCAGAACTTAAGCCCCAG6325870.8442091102764606No Hit
TTCCGTGGAGGACCGAACCACGTTGGTG6286040.8388936597752157No Hit
ACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTG5930060.791386904328737No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAT4448090.5936129103711619No Hit
CGATGAGTACTAGGTGTAGGAGG4044070.5396950516839149No Hit
TCACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC4016580.5360264166279463No Hit
AAAAACTGCCCCCAGTTCGGATTGCAGGC3309710.44169218374280605No Hit
CGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC3241120.4325386123172374No Hit
AATGGATGTATGTCTAAGCATCGAGTCAGG3150870.4204944393888574No Hit
ACATGGAGACTGATTTAAAGGAGT3048430.40682346903114847No Hit
AAGGTCAGAACTTAAGCCCCAGT3016990.4026276928885638No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACTAC2850030.38034630660466007No Hit
TGATGAGGTGTTATGGGGAACCCA2844470.3796043054802081No Hit
AAGGTCAGAACTTAAGCCCCAGTA2795970.3731318136572006No Hit
ATGAGTACTAGGTGTAGGAGG2582270.3446128136040728No Hit
GGCTCCGCCGGAGAAGGTAAAAGCCCTGTAAATGAAAAC2518920.3361585382022682No Hit
ATTCCGTGGAGGACCGAACCACGTTGGTG2498760.33346811685893146No Hit
TGATGAGGTGTTATGGGGAATCC2489770.3322683704364812No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAG2477120.3305801844249133No Hit
TGATGAGGTGTTATGGGGAACCCC2262930.3019957921863572No Hit
TGATGAGGTGTTATGGGGAACCC2216350.295779530967477No Hit
ACAACGCTAGGTACTGCAAACTG2211880.29518299409224313No Hit
TGATGAGGTGTTATGGGGAACCCGCAAGGGGAAGTACCTGAT2178250.29069495491682573No Hit
TTGTTCCTGGGGTTGCGGATAG2149050.2867981144790563No Hit
ACTGCAGTTTAGCACTGAATTTCGGTATTGTCTG2147440.28658325444122035No Hit
TGATGAGGTGTTATGGGGAACCCGC2102690.28061121301689906No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACT2044580.2728562336388585No Hit
AAAAAGGGTGATGAATATTCAGG2035350.27162445839089233No Hit
TGATGAGGTGTTATGGGGAATCCA1979180.2641283786857721No Hit
AATGATGAGGTGTTATGGGGAATCCG1975730.2636679643189809No Hit
GGATGAGGTGTTATGGGGAACCCG1949700.2601941712849008No Hit
ATGATGAGGTGTTATGGGGAACCCGC1925980.25702865569641137No Hit
AATTCTAACCGGGCTCCATGAAACTGGAG1889920.2522163246626454No Hit
TACTGCAGTTTAGCACTGAATTTCGGTATTGTCTG1857770.2479257965779095No Hit
GCGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC1814120.24210055393720276No Hit
AATGGATGTATGTCTAAGCATCGAGTCAG1803330.24066059132338313No Hit
ACGCTAGGTACTGCAAACTG1776380.23706402112482539No Hit
AAACGATGAGTACTAGGTGTAGGAGG1765340.23559069515109338No Hit
AGGTGATGGACAATCGGTTGAAATTCCGATACCGCATAAAAGCG1735630.2316257934591026No Hit
AAAAACTGCCCCCAGTTCGGATTGCAGGCTG1709150.2280919463771802No Hit
TGATGAGGTGTTATGGGGAATCCC1571950.20978213445724975No Hit
ATTCTAACCGGGCTCCATGAAACTGGAG1564970.20885062944849525No Hit
TTGTTCCTGGGGTTGCGGATAGGTC1526070.2036592906461243No Hit
TGAGCACTCTAGCAAGACTGCCGCGG1514100.2020618529735181No Hit
TTGAGCACTCTAGCAAGACTGCCGCGG1510160.20153604642129858No Hit
GGATGAGGTGTTATGGGGAATCCG1460680.1949327702274344No Hit
ATGATGAGGTGTTATGGGGAATC1424800.19014446081280537No Hit
AGGACTGCACTCGGAGATGCTC1387020.18510258986284203No Hit
AACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTG1378750.18399892991693953No Hit
TGATGAGGTGTTATGGGGAAT1358960.18135788634627314No Hit
TGTTCCTGGGGTTGCGGATAGGTC1303610.17397123846166565No Hit
TGGAATTCTAACCGGGCTCCATGAAACTGG1224940.16347245636442856No Hit
TGTTCCTGGGGTTGCGGATA1089150.14535081379440412No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGGC1060960.1415887613306808No Hit
ACCGACCGCTGGTGAACCAG966690.12900810557490935No Hit
AATGGATGTATGTCTAAGCATC947370.12642978512087832No Hit
CCGGTGAAATCAGGAGTTCGATTCTCCTAGGGAC882690.11779801664433967No Hit
GGTGAAATCAGGAGTTCGATTCTCCTAGGGAC862940.11516231121125932No Hit
AATGATGAGGTGTTATGGGGAACCCGC860190.11479531425222281No Hit
AGGTCAGAACTTAAGCCCCAG859700.1147299220667945No Hit
ACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGC849080.11331264653771532No Hit
GATGAGTACTAGGTGTAGGAGG846000.11290160994359444No Hit
TGTTCCTGGGGTTGCGGATAGG831880.11101724737574155No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGG830860.1108811248672989No Hit
ACCTGTTAAGCTGACTGATACTAATAGGTCGAGGGCTTGACC804390.10734861231977295No Hit
AATGATGAGGTGTTATGGGGAATC796570.1063050064217128No Hit
AACGCTAGGTACTGCAAACTG762110.10170620089138623No Hit
AAGGACTGCACTCGGAGATGCTC754750.1007239835755649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)