FastQCFastQC Report
Tue 4 Jul 2023
SRR6286692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44431801
Total Bases4.4 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGATATGGCGGAACTGGCAGACGCGCTAGAGATCGGAAGAGCACACGT12000322.700840328304495No Hit
AGGGCGTAGATCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT8693551.9566053602013567Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCAGATCGGAAGAGCAC7202871.6211069184433915No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAGATCGGAAGAG7125421.6036757096566938No Hit
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAGATCGGAAGAG7072581.591783326541276No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAAGATCGGAAGA4804271.081268346516046No Hit
TTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACTCC4487481.0099703138299527Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCAGATCGGAAGAGCAC4310490.9701362319299187No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACCAGATCGGAAGAG4286620.964763953637621No Hit
AGCTAGTTATGAGAGTTCGATTCTCTTCACCCGCAGATCGGAAGAGCACA4166540.937738265437406No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACAGATCGGAAGAGCACA4101040.9229965717572421No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAA3861880.8691702593824634No Hit
TGTTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACT3765660.8475145988342899Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGGAATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACG3523820.7930851148707656No Hit
ACGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACT2755770.6202246899692407Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AAGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGA2707060.6092618212797631No Hit
AAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGAAGAGC2449490.551292080192743No Hit
AACCTTTATCTCCAGTTCAAATCTGGATGGCGCCAGATCGGAAGAGCACA2433900.5477833320328385No Hit
AGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGAA2389610.5378152463367398No Hit
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCAGATCGG2381790.5360552456561462No Hit
ATCATGTTGCCCGGGGTTCGATTCCCCGGTAAGCCACCAGATCGGAAGAG2318700.521855956277802No Hit
TGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAACTCC2221460.499970730423464Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCAGATCGGAAGAGCAC2206390.4965790155568981No Hit
AACGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAAC2099610.4725466788978462Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAAGATCGGAAGA1994050.4487889203500889No Hit
ACGGTGGTAACAGCAGTTCAAGTCTGCTAGGGGTCAGATCGGAAGAGCAC1988240.44748129836105455No Hit
TTGTTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAAC1915630.4311393994585095Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTGAGATCGGAAGAGCA1707530.38430357572046203No Hit
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCACCAAGA1643360.36986121719441445No Hit
AACCGGCGTACGGGTGACCGTACCAAGGGTTCGAATCCCTTTCTTTCCGC1640730.36926929880695136No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACCAAGATCGGAAGA1628840.36659328754195675No Hit
GGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCAGATCGGAAGAGCACA1595870.35917292661623146No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCTAGATCGGAAGAG1546010.34795123429725483No Hit
AATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCT1497750.3370896444193203Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAAAACTGCCCCCAGTTCGGATTGCAGGCAGATCGGAAGAGCACACGT1494130.33627491264646237No Hit
ATTCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGA1492310.3358652961197769No Hit
TCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCAGATCGGAAGAGCACA1442960.32475838645388244No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACAGATCGGAAGAGC1391350.31314283209001587No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAGATCGGAAGAG1353000.30451162670628634No Hit
ATCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAGATCGGAAGAG1338340.3012121880902374No Hit
ACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTGAACTCC1291850.29074896153770585Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCTCCGCCGGAGAAGGTAAAAGCCCTGTAAATGAAAACAGATCGGAAGA1287760.2898284496727918No Hit
AACAGGTATGTGTTGGTTCGAGTCCAGCATGGGCAGCCATGCCCATTCGC1278280.2876948427096169No Hit
TCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAGATCGGAAGAGC1249310.2811747378864971No Hit
TGGAATTCTAACCGGGCTCCATGAAACTGGAGATCGGAAGAGCACACGTC1243720.279916629983106No Hit
TTCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAG1183200.2662957551506859No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACTACAGATCGGAAGAGC1143310.2573179511674532No Hit
AAGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAGATCGGAA1142770.2571964165936015No Hit
ATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTG1137070.25591355164738877Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCACCAGAT1107000.24914587639605243No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAGATCGGAAGAGCACACG1098100.24714280656775536No Hit
AGGGCGTAGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAG1095240.24649912345439248TruSeq Adapter, Index 10 (100% over 36bp)
TTCCTGGGGTTGCGGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGT1067610.24028060442564547Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACATCTGTGAGAACACCCACCTATCGAGAGATAGGTGTCAAAATTAAAGC1054060.23723098687806962No Hit
TTCCTGGGGTTGCGGATAGACCTCAAAAACTGCCCCCAGTTCGGATTGCA1051120.23656929864265464No Hit
TTCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCAGATC1015670.22859077893331398No Hit
AGGGCGTAGATCGGGTAACCGGTGCGAGGGTTCAAATCCCTCTTTCTCCG1013500.22810238999765056No Hit
TCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAGA983590.22137072499041846No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCATTCCTGGGGTT976270.21972325632265052No Hit
TGATGAGGTGTTATGGGGAACCCGAGATCGGAAGAGCACACGTCTGAACT974430.219309138515452Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGTAGATCGGAAGAGCAC970500.2184246368946422No Hit
AGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAGATCGG968810.2180442786912914No Hit
CGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTC964170.21699998161226908Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGATGAGGTGTTATGGGGAATCCGAGATCGGAAGAGCACACGTCTGAACT961190.21632929081582805Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTGGGGTTGCGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG953720.21464806254421243Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCATGATGAGGTGT925780.2083597736675135No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAGGAGATCGGAAGAGCACACGT920380.20714442792899618No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAAGATCGGAAGA901000.20278268711187286No Hit
TCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAGATCGGAAGAGC872620.19639537006388733No Hit
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACTAGATCGGAAGAG872610.19639311942363083No Hit
ACCGGCGTACGGGTGACCGTACCAAGGGTTCGAATCCCTTTCTTTCCGCC868570.19548386075999938No Hit
GCGGATATGGCGGAACTGGCAGACGCGCAGATCGGAAGAGCACACGTCTG858780.19328048394887257Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCATGTTGCCCGGGGTTCGATTCCCCGGTAAGCCACCAAGATCGGAAGA846000.19040416570104823No Hit
AATGGATGTATGTCTAAGCATCGAGTCAGGTATACCAGGAAAATCCGGTA843320.18980099411230258No Hit
AGGGCGTAGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACT836350.18823229785351261Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
AACTCCGAATGCCATATACTTGAATTCCGGCAGTCAGACTGCGAATGAGA836130.18818278376786934No Hit
AGTGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAACT834880.18790145373580514Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCACCAA829350.18665684967395313No Hit
ATCCAGAGGCCGGGAGTTCGAATCTCCCTGGGTGCACCAGATCGGAAGAG827760.18629899787316748No Hit
TCAAAAACTGCCCCCAGTTCGGATTGCAGGCTGAGATCGGAAGAGCACAC825880.18587587750494292No Hit
GGCCAGATAGCTCAGTCGGTAGAGCAGAGGACAGATCGGAAGAGCACACG825250.18573408716878256No Hit
GCGGATATGGCGGAACTGGCAGACGCGCTAGATCGGAAGAGCACACGTCT818900.18430493060589645Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGATATGGCGGAACTGGCAGACGCGCTAAAGATCGGAAGAGCACACGT813620.18311659255045729No Hit
TTGTCGTGCTAGATGGGGAGTTAGCGGTGCCCTGTAACCTGCAATCCGCT791940.17823720447433586No Hit
ACCCGAAGGTCGGAGGTTCAAGTCCTTCCCCCGCAACCATTCCTGGGGTT785960.17689132160094073No Hit
AGGTGGAGGTCACCAGTTCAATCCTGGTCATGGGCAGATCGGAAGAGCAC774210.17424681929953728No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGTAGATCGGAAGAGCAC757690.17052876159577687No Hit
AGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAGATCGGAAG752070.16926390177161624No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACTAGATCGGAAGAG739810.1665046168171306No Hit
ATACATCTGTGAGAACACCCACCTATCGAGAGATAGGTGTCAAAATTAAA729920.16427873360343867No Hit
TCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCAGATCG721680.1624242060320715No Hit
TTCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCACCAG715080.16093878346277254No Hit
GCGTTATTAGCTCAGTTGGTAGAGCATCTGACAGATCGGAAGAGCACACG698860.1572882449667075No Hit
GGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAAGATCGGAAGAG696390.15673233682334867No Hit
TCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAGATCGGAAGAGC694120.15622144148512007No Hit
AACCGTAGGTTGTAGGTTCGAGACCTACTACCCCTGAGATCGGAAGAGCA669130.15059709148409267No Hit
ATCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAAGATCGGAAGA667310.1501874749574072No Hit
TAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTGAACT659200.1483622057093747Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ACCATGAGGTCGCAGGTTCAATCCCTGTCACCCCGACCAGATCGGAAGAG653880.14716486509290946No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCAAGATCGGAAGAG646210.14543862401616356No Hit
ATCAGGTTGCCCGGGGTTCGAGTCCCCGATGGCGCACCATGCCCATTCGC644690.1450965266971735No Hit
GCAGGTGTGGCGGAATTGGCAGACGCACAGATCGGAAGAGCACACGTCTG640110.1440657334596903Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCTAGTTATGAGAGTTCGATTCTCTTCACCCGTAGATCGGAAGAGCACA638920.14379790726916516No Hit
GCAGGTGTGGCGGAATTGGCAGACGCACTAGAGATCGGAAGAGCACACGT636930.14335002985811895No Hit
AGGTGATGGACAATCGGTTGAAATTCCGATACCGCATAAAAGCGAGATCG635270.1429764235755377No Hit
ACCCGAAGGTCGGAGGTTCAAGTCCTTCCCCCGCAACCAGATCGGAAGAG632270.14230123149858365No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACTAGATCGGAAGAGCAC631640.14215944116242327No Hit
ACTCCGAATGCCATATACTTGAATTCCGGCAGTCAGACTGCGAATGAGAT631300.14208291939370182No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCAAGATCGGAAGAGCA620970.13975800800872332No Hit
GGCCAGATAGCTCAGTCGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA619960.13953069334281543No Hit
AACAGGTATGTGTTGGTTCGAGTCCAGCATGGGCAGCCAGCGCCATTAGC617820.13904905632792153No Hit
AACGATGAGTACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTC604330.1360129426218847Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCGGTGGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCAC600080.13505642051286645No Hit
TACATCTGTGAGAACACCCACCTATCGAGAGATAGGTGTCAAAATTAAAG598590.13472107511464593No Hit
CTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTGAGATCGGAAGAGCACA594700.13384557605486214No Hit
GCAGGTGTGGCGGAATTGGCAGACGCACTAGACAGATCGGAAGAGCACAC590900.13299033275738698No Hit
GCGTTATTAGCTCAGTTGGTAGAGCATCTGAGATCGGAAGAGCACACGTC580940.13074869506189946No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCATGTTCCTGGGG571380.1285970829766725No Hit
ATCCAGAGGCCGGGAGTTCGAATCTCCCTGGGTGCAGATCGGAAGAGCAC563870.1269068521440308No Hit
ACATGGAGACTGATTTAAAGGAGTAGATCGGAAGAGCACACGTCTGAACT556180.12517610978677185Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCATTCCTGGGGTT542460.12208823135483524No Hit
ACGATGAGTACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTCC534710.12034398515603723Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
CAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCAGATCGGAAGAGCACAC533200.12000413847730368No Hit
TGTTCCTGGGGTTGCGGATAGAGATCGGAAGAGCACACGTCTGAACTCCA518090.11660342104971168Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CGGTGAAATCAGGAGTTCGATTCTCCTAGGGACAGATCGGAAGAGCACAC516310.11620280708405226No Hit
AACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTGAACTC509830.11474439219783146Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AAATGGATGTATGTCTAAGCATCGAGTCAGGTATACCAGGAAAATCCGGT508670.1144833179280759No Hit
AACCGTAGGTTGTAGGTTCGAGACCTACTACCCCTGCCAAGGGGTATAGC507960.11432352246986344No Hit
AGGGCGTAGATCGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTA504880.11363032527085723TruSeq Adapter, Index 10 (100% over 35bp)
GCTCCTATGGCTCAGATGGTAGAGCAACTGAAGATCGGAAGAGCACACGT503960.113423266367258No Hit
ACCCGAAGGTCGGAGGTTCAAGTCCTTCCCCCGCAGATCGGAAGAGCACA502730.11314643761570682No Hit
TTCCTGGGGTTGCGGATAGTCAAAAACTGCCCCCAGTTCGGATTGCAGGC497700.1120143655666805No Hit
GCGCCATTAGCTCAGTTGGTAGAGCATCTGACAGATCGGAAGAGCACACG483210.10875318783499233No Hit
TCACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACAGATCGGAAGAGCA477090.10737579599800601No Hit
AGGACTGCACTCGGAGATGCTCAGATCGGAAGAGCACACGTCTGAACTCC475870.10710121788671137Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCCAGAGGCCGGGAGTTCGAATCTCCCTGGGTGCACCAAGATCGGAAGA474070.10669610264053893No Hit
AACCTTTATCTCCAGTTCAAATCTGGATGGCGCTAGATCGGAAGAGCACA472330.10630449123590557No Hit
TCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCACCAAG471320.1060771765699977No Hit
GCGCCATTAGCTCAGTTGGTAGAGCATCTGAAGATCGGAAGAGCACACGT466560.10500587180789724No Hit
TTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC465210.1047020353732679Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AGCGTGAGGTCGATGGTTCGAGTCCATTTAAGCCCAGATCGGAAGAGCAC462470.10408535994298318No Hit
AGTTGGCCGTCGCGGGTTCGAGTCCCGTTTCCCGCAGATCGGAAGAGCAC456780.10280474563702695No Hit
GGTGGTAACAGCAGTTCAAGTCTGCTAGGGGTCAGATCGGAAGAGCACAC456200.10267420850214917No Hit
TCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAAGATCGGAAGAG447470.10070939955821281No Hit
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCAGATCGGAAGAGCAC446570.1005068419351266No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)