FastQCFastQC Report
Tue 4 Jul 2023
SRR6286693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6286693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50618624
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACG24005634.742450130608054No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAA10497392.0738197071496844No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCAGATCGGAAGAGCAC9314391.840111260234968No Hit
TTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACTCC8528171.6847889820157895Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAGATCGGAAGAG8124611.6050633853658292No Hit
AGGGCGTAGATCGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG7000841.3830561652564874Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TTCCTGGGGTTGCGGATAGAGATCGGAAGAGCACACGTCTGAACTCCAGT6566121.2971747315770574Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGGAATTCTAACCGGGCTCCATGAAACTGGAGATCGGAAGAGCACACGTC6512911.2866627903595325No Hit
GCGGATATGGCGGAACTGGCAGACGCGCTAGAGATCGGAAGAGCACACGT6108141.2066981512575292No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCAGATCGGAAGAGCAC4841950.9565550418754962No Hit
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAAGATCGGAAGA4613690.9114609674099399No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACAGATCGGAAGAGCACA4212980.8322984046346262No Hit
ACGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACT4009340.7920681526230346Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTCCTGGGGTTGCGGATAGTGGAATTCTAACCGGGCTCCATGAAACTGGA3946630.7796794318233542No Hit
AGCTAGTTATGAGAGTTCGATTCTCTTCACCCGCAGATCGGAAGAGCACA3804990.751697636032145No Hit
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAGATCGGAAGAG3650690.721214784503032No Hit
AACGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAAC3548220.7009712472626676Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGTTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAACT3491820.6898291032170293Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGATGAGGTGTTATGGGGAACCCGAGATCGGAAGAGCACACGTCTGAACT3133600.6190606840675875Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACCAGATCGGAAGAG2876590.5682868819191924No Hit
TTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAGTC2843060.5616628377729114Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
AAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGAAGAGC2808130.5547622155829444No Hit
AACCTTTATCTCCAGTTCAAATCTGGATGGCGCCAGATCGGAAGAGCACA2760770.545405975476536No Hit
ACGGTGGTAACAGCAGTTCAAGTCTGCTAGGGGTCAGATCGGAAGAGCAC2444220.48286970424166414No Hit
GGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCAGATCGGAAGAGCACA2440770.48218813691972345No Hit
TTCCTGGGGTTGCGGATAGGAGATCGGAAGAGCACACGTCTGAACTCCAG2179210.4305154561293488Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACCGGCGTACGGGTGACCGTACCAAGGGTTCGAATCCCTTTCTTTCCGC2064770.40790717661546866No Hit
AACTCCGAATGCCATATACTTGAATTCCGGCAGTCAGACTGCGAATGAGA2061400.4072414137531672No Hit
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCAGATCGG2046720.4043412954093734No Hit
TTCCTGGGGTTGCGGATAGTCAAAAACTGCCCCCAGTTCGGATTGCAGGC1953820.3859883666533488No Hit
ACTCCGAATGCCATATACTTGAATTCCGGCAGTCAGACTGCGAATGAGAT1792150.3540495292799741No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAAGATCGGAAGA1790020.35362873554208035No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCTAGATCGGAAGAG1727080.34119457692093724No Hit
AAGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGA1700260.3358961318268944No Hit
AATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCT1628130.3216464359046978Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGTTCCTGGGGTTGCGGATAGAGATCGGAAGAGCACACGTCTGAACTCCA1626030.32123156883916876Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAAGATCGGAA1585170.31315944107844573No Hit
TGTTAATTGAAGAGAGCTGGAAAGATCGGAAGAGCACACGTCTGAACTCC1561400.30846354100814755Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATTCTAACCGGGCTCCATGAAACTGGAAAGATCGGAAGAGCACACG1515750.2994451212265272No Hit
AGGGCGTAGATCGGGTAACCGGTGCGAGGGTTCAAATCCCTCTTTCTCCG1512200.2987437983300376No Hit
TTCCTGGGGTTGCGGATAGACCTCAAAAACTGCCCCCAGTTCGGATTGCA1411870.2789230303850219No Hit
TTGTTCCTGGGGTTGCGGATAGGTCAGATCGGAAGAGCACACGTCTGAAC1362900.2692487255283747Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CGATGAGTACTAGGTGTAGGAGGAGATCGGAAGAGCACACGTCTGAACTC1338360.2644007075340491Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTG1326460.2620497941627177Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAGATCGGAAGAG1301620.2571425094447451No Hit
AAGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAGATCGGAA1288550.25456045585119025No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCATGATGAGGTGT1280890.2530471788407366No Hit
ATCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAGATCGGAAGAG1277430.25236363596134104No Hit
TTCCTGGGGTTGCGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1259250.2487720725083321Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGTAGATCGGAAGAGCAC1236470.244271752626069No Hit
ATCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAAGATCGGAAGA1205040.2380625755453171No Hit
AACGATGAGTACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTC1134210.2240697020922576Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTTCCTGGGGTTGCGGATAAGATCGGAAGAGCACACGTCTGAACTCCAG1114060.2200889538206333Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AAGGTCAGAACTTAAGCCCCAAGATCGGAAGAGCACACGTCTGAACTCCA1087390.21482014208841393Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCATTCCTGGGGTT1085420.21443095726979855No Hit
TGATGAGGTGTTATGGGGAATCAGATCGGAAGAGCACACGTCTGAACTCC1082270.21380865667150495Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCAGATC1082230.21380075444168536No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACAGATCGGAAGAGC1068280.2110448517920993No Hit
GGCTCCGCCGGAGAAGGTAAAAGCCCTGTAAATGAAAACAGATCGGAAGA1067420.21087495385097785No Hit
ATCATGTTGCCCGGGGTTCGATTCCCCGGTAAGCCACCAGATCGGAAGAG1054100.20824351132105054No Hit
AAGGTCAGAACTTAAGCCCCAGAGATCGGAAGAGCACACGTCTGAACTCC1050530.2075382373096511Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCAGATCGGAAGAGCAC1039050.20527029735142543No Hit
TCAAAAACTGCCCCCAGTTCGGATTGCAGGCAGATCGGAAGAGCACACGT1037870.2050371815717472No Hit
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCACCAAGA1009810.19949376735329669No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGAGATCGGAAGAGCACACG1002690.1980871704454076No Hit
ACCGGCGTACGGGTGACCGTACCAAGGGTTCGAATCCCTTTCTTTCCGCC990860.19575008597626045No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCATTCCTGGGGTT976480.19290923435611368No Hit
AAATGGATGTATGTCTAAGCATCGAGTCAGGAGATCGGAAGAGCACACGT951450.187964414046498No Hit
ACGATGAGTACTAGGTGTAGGAAGATCGGAAGAGCACACGTCTGAACTCC938480.18540211602749218Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AGCGAAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAGATCGGAAG932790.18427802383565386No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAGATCGGAAGA907770.17933517908349306No Hit
TCAAAAACTGCCCCCAGTTCGGATTGCAGGCTGAGATCGGAAGAGCACAC906400.1790645277121717No Hit
ATTCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGA893100.17643703629715418No Hit
AAGGTCAGAACTTAAGCCCCAGTTCCTGGGGTTGCGGATAGGTCAGATCG891860.17619206717274655No Hit
TCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCACCAGATCGGAAGAGC886190.175071926095818No Hit
AGGTGGAGGTCACCAGTTCAATCCTGGTCATGGGCAGATCGGAAGAGCAC859770.16985250329997117No Hit
ACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTGAACTCC855190.16894769798562678Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCTAGTGAGGGAAACTTCATGCAGGTTCAAGTCCTGTTATCCGCAGATCG842840.1665078845288248No Hit
AAGGTCAGAACTTAAGCCCCAGTTCCTGGGGTTGCGGATAGAGATCGGAA837420.1654371323882688No Hit
GGCCAGATAGCTCAGTCGGTAGAGCAGAGGACAGATCGGAAGAGCACACG837020.16535811009007279No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGTAGATCGGAAGAGCAC836130.16518228547658664No Hit
ACATGGAGACTGATTTAAAGGAGTAGATCGGAAGAGCACACGTCTGAACT813290.16067011224959415Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAGATCGG760220.15018582883643775No Hit
TCAGTAGGTCGTGGGTTCGATTCCTACTGCCAGCAGATCGGAAGAGCACA746640.14750302181268302No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCACCAAGATCGGAAGA743790.1469399879380364No Hit
TTCCTGGGGTTGTGGATAGGTCAGATCGGAAGAGCACACGTCTGAACTCC727830.14378699824001537Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGAATTCTAACCGGGCTCCATGAAACTGAAGATCGGAAGAGCACACGTC726570.14353807800069793No Hit
TTCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCAAGATCGGAAGAG724280.14308567534352573No Hit
ACCGGCGTACGGGTGACCGTACCAAGGGTTCGAATCCCTTTCTTTCCGCA718490.1419418275771384No Hit
ACCGGTGGGTCCGGGGTTCGAGTCCCTGAAGGTCCAGATCGGAAGAGCAC715680.1413866959323114No Hit
TTCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAG715620.14137484258758198No Hit
AAGGTCAGAACTTAAGCCCCAGTTCCTGGGGTTGCGGATAAGATCGGAAG710210.14030606600448087No Hit
AGGGCGTAGATCGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG701260.13853794208234502Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCTAGCGCTTTACGGCGTGGGGGTTCGACTCCCTTCACCTGCACCAGAT700490.1383858241583177No Hit
AAGGTCAGAACTTAAGCCCCAGTAAACGGCGGCCGTAGATCGGAAGAGCA699570.13820407287246686No Hit
TGTTAATTGAAGAGAGCTGGAAGGCTCCGCCGGAGAAGGTAAAAGCCCTG697900.1378741547774985No Hit
ACATCTGTGAGAACACCCACCTATCGAGAGATAGGTGTCAAAATTAAAGC674660.13328295925230998No Hit
GGTGAACGTCGCGAGTTCGAATCTCGTCTTCCGCTCCAAGATCGGAAGAG674530.13325727700539627No Hit
TCCTAGCTTAGGCTGTAAAATGTGAGGTCTGGCCCATAAAAGTGGCGAGA673110.13297674784680044No Hit
AGGGCGTAGATCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAT670710.13250261405762434TruSeq Adapter, Index 9 (100% over 36bp)
GCGGATATGGCGGAACTGGCAGACGCGCAGATCGGAAGAGCACACGTCTG668500.13206601586009134Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTCGTGCTAGATGGGGAGTTAGCGGTGCCCTGTAACCTGCAATCCGCT662220.1308253657784139No Hit
TGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAAAGATCGGAA657670.1299264871364342No Hit
AACAGGTATGTGTTGGTTCGAGTCCAGCATGGGCAGCCATGCCCATTCGC653660.12913428859701914No Hit
AACCGTAGGTTGTAGGTTCGAGACCTACTACCCCTGAGATCGGAAGAGCA636860.1258153520727865No Hit
ACGGTGAAATCAGGAGTTCGATTCTCCTAGGGACTACAGATCGGAAGAGC631080.12467347986385408No Hit
ACCTCAAAAACTGCCCCCAGTTCGGATTGCAGGCTGAGATCGGAAGAGCA617700.12203018398919732No Hit
TCATGTTGTCCGGGGTTCGATTCCCCGGTGAGCCACCAGATCGGAAGAGC616070.12170816812404857No Hit
ACTGCAGTTTAGCACTGAATTTCGGTATTGTCTGAGATCGGAAGAGCACA614820.12146122344218603No Hit
GCAGGTGTGGCGGAATTGGCAGACGCACAGATCGGAAGAGCACACGTCTG577090.11400744516484684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCGTGTGTAGGGGCAACTCTACCGGGGGTTCGAATCCCCCTCTCTCCGC573730.11334365786000031No Hit
AGCCGTGTGCCGGGGGTTCGAATCCCTTAAGACGCAAGATCGGAAGAGCA572010.11300386197775744No Hit
GGTGGTAACAGCAGTTCAAGTCTGCTAGGGGTCAGATCGGAAGAGCACAC566480.11191137870519752No Hit
ACCCGAAGGTCGGAGGTTCAAGTCCTTCCCCCGCAGATCGGAAGAGCACA556390.10991804123320302No Hit
AGCTAGTTATGAGAGTTCGATTCTCTTCACCCGTAGATCGGAAGAGCACA544620.10759281010878526No Hit
AGTTGGCCGTCGCGGGTTCGAGTCCCGTTTCCCGCAGATCGGAAGAGCAC541130.10690334055702502No Hit
ACCATGAGGTCGCAGGTTCAATCCCTGTCACCCCGACCAGATCGGAAGAG533120.1053209190356498No Hit
AACCTTTATCTCCAGTTCAAATCTGGATGGCGCTAGATCGGAAGAGCACA532550.10520831226072049No Hit
TAACCGGGCTCCATGAAACTGGAGAGATCGGAAGAGCACACGTCTGAACT531240.10494951423412853Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TCTGGTTGTCGTGGGTTCGAGCCCCATCGTTCACCCCAGATCGGAAGAGC530850.10487246749338743No Hit
TTCCTGGGGTTGCGGATAGGTTAGATCGGAAGAGCACACGTCTGAACTCC529980.10470059399481108Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GGCCAGATAGCTCAGTCGGTAGAGCAGAGGACTGAGATCGGAAGAGCACA526350.10398346663868224No Hit
CTGATAGTACTGGAATTCTAACCGGGCTCCATGAAACTGGAGAGATCGGA525590.1038333242721098No Hit
AAGGTCAGAACTTAAGCCCCAGTGTTCCTGGGGTTGCGGATAAGATCGGA521380.10300161458359673No Hit
TGATGAGGTGTTATGGGGAACCCAAGATCGGAAGAGCACACGTCTGAACT507060.10017261630817938Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)