FastQCFastQC Report
Tue 4 Jul 2023
SRR6322030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6322030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80787331
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length69
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34316574.247766274145138Illumina PCR Primer Index 6 (96% over 29bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC3932600.48678424591103275No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2466200.3052706370507524No Hit
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT2012880.24915787847973342No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1903730.23564709669638673No Hit
TCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC1847070.22863362078393207No Hit
CGGCACGCAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1704120.21093901468288387No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATATCTC1573260.19474093035701354No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT1433920.1774931764981814No Hit
TAGACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATC1285060.15906702005045817No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATAT1281630.15864244852946066No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATAT1253100.15511095421632384No Hit
TCGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATAT1237760.153212141641367No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA1131860.1401036506577993No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA1123410.1390576945783739No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATATCT1086500.13448890891073007No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT1059910.13119755125961519No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC997850.1235156537106047No Hit
ATGAAAGCTACTAAACTGGTACTGGGCGCTGTAGGCACCATCAATATCTC997350.12345376281833101No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC993590.12298834330843286No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA980130.1213222404884251No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA954490.11814847553263023Illumina PCR Primer Index 6 (96% over 29bp)
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC946940.11721392305929751No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA945560.1170431041966221No Hit
AAGTAGGTAGCTTAACCTTCGGGAGGGCTGTAGGCACCATCAATATCTCG937620.1160602768273159No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT909710.1126055272205985No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT891560.11035888783106351No Hit
GACTTCGAAGGTTCGAATCCTTCCCCCACCACCACTGTAGGCACCATCAA860300.10648946924611237No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCT835100.10337016827551834No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)