FastQCFastQC Report
Tue 4 Jul 2023
SRR6322031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6322031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84991718
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length69
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA52268076.149783911886567Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT27280573.2097915705151414No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG9095391.070150152747824No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC3554650.4182348684844799No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC3382470.3979764240087487No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT3255960.3830914442746057No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG3031930.3567324053856636No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC2908080.34216039732247794No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC2894390.3405496521437536No Hit
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC2794720.3288226271646844No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC2642480.3109102936359046No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT2524680.2970501196363627No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATATCT2346670.2761057259720294No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATAT2334940.274725591498221No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA2180730.25658147067929604No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG2141500.251965726825289No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC2014400.23701132856262536No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC1891840.22259109999400178No Hit
ACGATGTCGACTTGGAGGTTGTGCCCTGTAGGCACCATCAATATCTCGTA1877220.22087093238896527No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATA1727550.20326098126408035No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCT1700800.20011361577606893No Hit
TGCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATAT1620250.1906362217551597No Hit
TTTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCAT1579880.18588634718502808No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT1571180.18486271803565613No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1492550.17561122837874626Illumina PCR Primer Index 6 (96% over 29bp)
GCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT1455120.17120726986598858Illumina PCR Primer Index 6 (95% over 21bp)
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT1445040.17002127195499214No Hit
ATGTGGGAAGGCCCAGACAGCCAGGATGCTGTAGGCACCATCAATATCTC1378140.16214991677189064No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT1376760.16198754801026613No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA1365280.16063682816718683No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATATCTC1338060.15743416317340475No Hit
GCCGACTCAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1305140.15356084459899963No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC1287500.15148534825475585No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATC1271100.14955574847892827No Hit
CGGCACGCAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1226280.144282293481819No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT1158420.13629798611671787No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACTGTAGGCACCATCAATAT1123890.1322352373204175No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC1113930.131063358432171No Hit
ACTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1110830.13069861701113042Illumina PCR Primer Index 6 (96% over 29bp)
TTGTCGGGGGTCGGAGGTTCAAATCCTCTCGTGCCGACCACTGTAGGCAC1089510.12819013730255457No Hit
TCCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC1056180.1242685787337538No Hit
AGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTGTAGGCACCATC1021990.12024583383524498No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATCAA1012900.11917631786193567No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCG1012090.11908101445837346No Hit
AGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCCTGTAGGCACCAT1011420.11900218324801953No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA974840.11469823447974072No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA962160.11320632440916184No Hit
TTTCCCTGGTTCGAATCCAGGTACCCCAGCCACTGTAGGCACCATCAATA961680.11314984831816201No Hit
CGGTGGTTCAAGTCCACTCAGGCCCTGTAGGCACCATCAATATCTCGTAT954030.11224976061785219No Hit
TTTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATC949290.11169205921922887No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATATCTCGTA947160.11144144656541713No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATAT915630.1077316733378657No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATAT913940.10753283043413712No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATATCTCGTAT874260.10286414024481773No Hit
TCGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATAT857770.1009239512019277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)