FastQCFastQC Report
Mon 10 Jul 2023
SRR6322032.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6322032.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99556339
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length69
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA34583153.473726570037896Illumina PCR Primer Index 6 (96% over 29bp)
AAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGT18028791.8109133161274642No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATCTC7599170.7633034798517451No Hit
ATGTACACGGAGTCGACCCGCAACGCGACTGTAGGCACCATCAATATCTC7480140.7513474355460178No Hit
ACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTCG5966880.5993470692006865No Hit
ACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCGT5236750.5260086954382683No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGCTGTAGGCACCATCAATATC4238560.4257448639207193No Hit
AGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAAT4012220.4030099981880611No Hit
ACCGGAGTGGACGCATCACTGGTGTTCGGGTCTGTAGGCACCATCAATAT3487330.3502870871939154No Hit
ACCTGCGGTTGGATCACCTCCCTGTAGGCACCATCAATATCTCGTATGCC3307300.33220385896271254No Hit
ACCTGCGGTTGGATCACCCTGTAGGCACCATCAATATCTCGTATGCCGTC3166650.3180761799607758No Hit
TTTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCAT3096210.31100078921142327No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCTGTAGGCACCAT2858200.28709372288187496No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTCGGCCTGTAGGCACCATCAAT2627860.26395707459672657No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGACTGTAGGCACCATCAATATCT2548650.25600077560103934No Hit
AGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATCA2377180.23877736203216554No Hit
CGCAGGTTCGAATCCTGCACGACCCACCACTGTAGGCACCATCAATATCT2356930.23674333786018387No Hit
AGTAGGTAGCTTAACCTTCGGGAGGGCGCCTGTAGGCACCATCAATATCT2327520.23378923164299967No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTCTGTAGGCACCATCAAT2251890.22619252803179113No Hit
TACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCTC2140970.2150510978512378No Hit
TAGGCTTGTAGCTCAGGTGGTTAGAGCGCACCCCCTGTAGGCACCATCAA2088060.20973651913817362No Hit
TGGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATA2001970.2010891541522032No Hit
TAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1996810.20057085465949084No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCA1980350.19891751945599365No Hit
AGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCCTGTAGGCACCAT1954570.1963280309051943No Hit
TTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATC1939660.19483038644078707No Hit
AGGTTAATGAGGCGAACCGGGGGAACTGCTGTAGGCACCATCAATATCTC1843140.18513537344919845No Hit
AACGATGTCGACTTGGAGGTTGTGCCCCTGTAGGCACCATCAATATCTCG1805220.18132647485159134No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGTAGGCACC1693400.17009464359672768No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTTAAGTGGCTGTAGGCACCATC1668050.167548346670321No Hit
AGACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATAT1661770.1669175480629114No Hit
GCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCGTATGCCGTCT1625750.16329949617773712Illumina PCR Primer Index 6 (95% over 21bp)
CGGCACGTAGCGCAGCCTGGTAGCGCACCGCTGTAGGCACCATCAATATC1615480.1622679194742185No Hit
AGGTAACATCAAATCGTACCCCAAACCGCTGTAGGCACCATCAATATCTC1570330.15773279891298533No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCTCTGTAGGCACCATCAATAT1559480.15664296373935566No Hit
TAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAAT1527890.15346988603106426No Hit
TCCTCTGTAGTTCAGTCGGTAGAACGGCGGACCTGTAGGCACCATCAATA1506690.15134043850286621No Hit
TCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCATCAA1491730.14983777175655286No Hit
ATTTCGGACGCGGGTTCAACTCCCGCCAGCTCCACCACTGTAGGCACCAT1475170.1481743919892434No Hit
ACTTGCCCCGAGATGAGTTCTCCCTGACCCCTGTAGGCACCATCAATATC1446440.1452885888059825No Hit
AAAGGCTGAGGCGTGATGACGAGGCACCTGTAGGCACCATCAATATCTCG1424780.14311293628424807No Hit
AAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCAA1373820.13799422656552285No Hit
ATGTGGGAAGGCCCAGACAGCCAGGATGCTGTAGGCACCATCAATATCTC1323830.13297294911577656No Hit
AGCGCACGCAGGCGGTTTGTTAAGTCAGATGCTGTAGGCACCATCAATAT1317980.13238534213275963No Hit
ATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCACTGTAGGCACCATCAAT1292700.12984607640102153No Hit
AGCAGTGGGAGCACGCTTAGGCGTGTGACTGCGTACCTGTAGGCACCATC1289570.12953168155369793No Hit
AGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCCTGTAGGCACCA1233110.12386052082529873No Hit
GCCGACTTAGCTCAGTAGGTAGAGCAACTGCTGTAGGCACCATCAATATC1216720.12221421681647011No Hit
GCGAAGGTGGCGGAATTGGTAGACGCGCCTGTAGGCACCATCAATATCTC1197590.12029269175918574No Hit
TTACGCTTTAGCAGCTTAATAACCTGCTTCTGTAGGCACCATCAATATCT1182240.11875085121400457No Hit
ACCTGCGGTTGGATCACCTCCTGTAGGCACCATCAATATCTCGTATGCCG1170500.11757161942244582No Hit
CGGAGGTTCGAATCCTCCCGGATGCCTGTAGGCACCATCAATATCTCGTA1164050.11692374505655537No Hit
ACCTGCGGTTGGATCACCTCCTTACTGTAGGCACCATCAATATCTCGTAT1159130.11642955251699241No Hit
CGGTGGTTCAAGTCCACTCAGGCCTCTGTAGGCACCATCAATATCTCGTA1150150.11552755068665191No Hit
GGGGCTGATTCTGGATTCGACGGGATTTGCGCTGTAGGCACCATCAATAT1144540.1149640506567844No Hit
ACCGCCAGCTAAGGTCCCAAAGTCATGGTCTGTAGGCACCATCAATATCT1123450.1128456521487798No Hit
TCAAACCGGGAGATAGCTGGTTCTCCCCGAAAGCTCTGTAGGCACCATCA1087710.10925572504227983No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTACCTCTGTAGGCACCATCAAT1066460.10712125523217562No Hit
ATGGCACTGCCCGGTAGCTAAATGCGGAAGCTGTAGGCACCATCAATATC1040290.10449259288250846No Hit
GCTGATATAGCTCAGTTGGTAGAGCGCACCCTCTGTAGGCACCATCAATA1024820.10293869886075262No Hit
TAGGGCTTACGGGTTCAAGTCCCGTCCTCGGTACCACTGTAGGCACCATC1019220.10237620328726632No Hit
TTGTAGGCACCATCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1007620.10121103388504474Illumina PCR Primer Index 6 (96% over 29bp)
AGCTCTTGATCGAAGCCCCGGTAAACGGCGGCCGTCTGTAGGCACCATCA1006230.10107141444805438No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)