FastQCFastQC Report
Wed 27 Sept 2023
SRR6359213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6359213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98548185
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA27473682.787842312874661Illumina Single End Adapter 1 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT17386141.7642273168196856No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA11618131.1789288661176256No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT9190070.9325458403926972No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATT8886850.9017771357229969No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT6028140.6116946750465267No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT5745850.5830498045194845No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT5333380.5411951524018428No Hit
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAAT5223040.52999859916243No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT5066860.5141505142890251No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATT5004790.5078520725673435No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC5003670.5077384225797765No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT4534070.4600866063641862No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA4309980.4373474762625004No Hit
GCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC4246420.4308978394680734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCTGTAGGCACCATCAATTC3860810.39176875758797586No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG3787240.38430337402966885No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC3734890.37899125184294363No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA3673760.37278819493225573No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT3106280.3152041815889354No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG2793090.28342378908348237No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA2785880.2826921672885198No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT2758170.2798803448282685No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG2521370.2558514903141037No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT2442620.2478604755632993No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCACCA2404920.2440349358032317No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA2400910.24362802825846058No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC2097020.21279133654262633No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCACC2075090.21056602919678327No Hit
GACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC2006210.20357655496141303No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT2002360.20318588312915148No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATTC1975940.2005049611010086No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1952900.1981670184996304Illumina Single End Adapter 1 (95% over 22bp)
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGCTGTAGGCACCATC1927970.19563729154423293No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGCTGTAGGCACCATCAA1915070.19432828722314874No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC1810450.18371216070595311No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1810030.18366954196061552No Hit
AGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1801840.18283847642653187No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATT1782100.18083539539566354No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT1769980.17960554017306357No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCAC1612810.16365699682850576No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA1602840.16264530899275315No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC1584950.16082995338777675No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTGTAGGCACCATC1578100.16013486194596077No Hit
TGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT1573050.1596224222698774No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1544540.15672942124707825No Hit
ACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATTC1460610.14821277530377652No Hit
AACATGGGTCAGTCGGTCCTGAGAGATGGGCGCTGTAGGCACCATCAATT1445040.1466328375301889No Hit
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG1416860.14377332266444076No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1406840.1427565611685289No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA1382460.14028264447488303No Hit
GACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATTCG1340370.13601163735283403No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATTC1292150.13111859949526214No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCTGTAGGCACCATCAA1286970.1305929683027648No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA1277520.12963404653266825No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1269840.12885473233220887Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA1268310.12869947833133608Illumina Single End Adapter 1 (95% over 22bp)
CGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTCGT1223050.12410680115519124No Hit
TGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCAA1216890.12348172622357276No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCTGTAGGCACCATCAATTCGT1203780.1221514125298198No Hit
TGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATC1199720.12173943132488944No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1193520.12111029746514357No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA1190320.12078558321495217No Hit
CCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCAATTCGTAT1178720.11960849405800829No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCATCAATTCG1160510.11776066702801274No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT1156430.11734665635901868No Hit
TGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1155880.11729084609726705No Hit
GAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA1155700.11727258092069377No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTC1144690.11615536095362894No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG1137950.11547143156416327No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1115350.11317813717218637Illumina Single End Adapter 1 (95% over 22bp)
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCTGTAGGCACCATCAATTC1102000.11182346990966906No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATTCGT1092340.11084323876690372No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACCATCAATTC1091670.11077525172076991No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC1069970.10857328321165936No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1064860.10805475514338495No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1062640.10782948463231463Illumina Single End Adapter 1 (95% over 22bp)
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT1055590.10711409854986167No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCA1039620.10549357149500015No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCA1032910.10481268630163No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG1031780.10469802158203116No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC1031780.10469802158203116No Hit
TTGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATC1024210.1039298694339221No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCTGTAGGCACCATCAATTCG1002450.10172181253262046No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCTGTAGGCACCATCAATTC1000100.10148335050513613No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG998190.10128953668705314No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAGGCACCATC995360.10100236752204012No Hit
TACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC993250.10078825906332015No Hit
ACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCGTA991240.10058429792491866No Hit
TCGGGGGCGGGCGGGCGTCCCGCGCGTGTGGGGGGACTGTAGGCACCATC989390.10039657249902674No Hit
GGCTGGGTCGGTCGGGCTGGGGCGCGAAGCGGGGCTGTAGGCACCATCAA988070.1002626278708228No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)