FastQCFastQC Report
Tue 26 Sept 2023
SRR6359214.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6359214.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105795920
Total Bases5.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA66120426.249808121144937Illumina Single End Adapter 1 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT18680131.7656758408074715No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT17183401.6242025212314426No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA12894861.2188428438450178No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT9740020.9206423083234211No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT8746820.8267634517474776No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATT8402300.794198868916684No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG7937960.7503087075569643No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT7184060.6790488706936904No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT5549000.5245003777083275No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG5544330.5240589618200777No Hit
GACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC4792320.4529777707873801No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT4582280.4331244531925239Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC4312120.4075884967964738No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT4143020.39160489364807266No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATT4117750.389216332728143No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA4028610.380790676993971No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA3701980.3499170856494277No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC3621990.3423563025870941No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA3449750.326075901603767No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC3369370.3184782551160763No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT3327640.3145338686028724No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA3268590.3089523679174017No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT3113490.294292067217715No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA3013330.2848247834131978Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2951400.2789710605097059Illumina Single End Adapter 1 (95% over 22bp)
AGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT2837080.26816535079991743No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC2804660.26510096041510867No Hit
GCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC2706600.2558321719778986No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG2568470.24277590288926076No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA2524630.2386320757927149Illumina Single End Adapter 1 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC2477930.23421791691021734No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCTGTAGGCACCATCAATTC2431820.22985952577377275No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT2338440.22103309844084726No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA2327460.21999525123464117Illumina Single End Adapter 1 (95% over 22bp)
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAAT2252880.21294583004713227No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAA2189190.2069257491215162Illumina Single End Adapter 1 (95% over 22bp)
TGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT2181580.2062064397190364No Hit
ACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATTC2138680.20215146292976138No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT2129990.20133007019552362No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC2108370.19928651312829454No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTGTAGGCACCATC2096870.1981995146882791No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA2065010.1951880564014189No Hit
TGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1980740.1872227208761926No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATTCG1811930.17126652899280048No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCACC1803990.17051602746117242No Hit
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG1792060.16938838473166073No Hit
CACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCGT1758720.16623703447165072No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1701110.16079164489519068Illumina Single End Adapter 1 (95% over 22bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCACCA1666300.15750134787806563No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGCTGTAGGCACCATC1635050.15454754776932797No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1634710.1545154104241449No Hit
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCATCA1619990.1531240524209251No Hit
TACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC1572830.14866641360082694No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG1553070.14679866671606998No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1538450.14541676087319813Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTCTGTAGGCACC1534810.14507270223653237No Hit
GTCAGGATGGCCGAGCGGTCTAAGGCTGCGTTCTGTAGGCACCATCAATT1487390.1405904877995295No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT1464960.13847036823348197No Hit
AGCAGAGTTGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC1438930.13600997089490785No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGAA1417990.13403068851804492Illumina Single End Adapter 1 (95% over 22bp)
TAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGACTGTAGGCACCATC1409950.1332707348260689No Hit
CACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGTA1376000.13006172638793634No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGC1373040.12978194244163668No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACCATCAATTC1342090.12685649881394293No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1324660.12520898726529342Illumina Single End Adapter 1 (95% over 22bp)
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA1321630.12492258680675021No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATTC1317200.12450385610333556No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGCTGTAGGCACCATCAA1307550.12359172262975736No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT1306200.12346411846505989No Hit
TCACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCA1294920.12239791477780995No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1277920.12079104751865667No Hit
CGGCGGATCTTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCA1270730.12011143718963832No Hit
CGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTCGT1265620.11962843179585754No Hit
GAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA1235910.11682019495647847No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTGTAGGCACCATC1223920.115686880930758No Hit
TGTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACCATCAATT1217900.11511786087781078No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCATCAATTCG1216690.11500348973760048No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATT1216070.11494488634344312No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACCTGTAGGCACCATCAA1207330.11411876752903136No Hit
CTCTAAGTCAGAATCCCGCCCAGGCGGAACGATACGCTGTAGGCACCATC1198910.1133228956277331No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTC1183130.11183134472482492No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCATC1156870.10934920741745051No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT1149450.10864785711963185No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCTGTAGGCACCATCA1147450.10845881391267262No Hit
ACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCGTA1138750.10763647596240006No Hit
CAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGC1132130.10701074294736507No Hit
CTCGCTTCTGGCGCCAAGCGTCCGTCCCGCGCTGTAGGCACCATCAATTC1129720.10678294588297922No Hit
TAGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGCTGTAGGCACCATCA1129560.10676782242642249No Hit
TGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTC1111050.10501822754601502No Hit
TTGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATC1108620.10478854004955956No Hit
GACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATT1103390.10429419206336123No Hit
TGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA1090440.10307013729830036Illumina Single End Adapter 1 (95% over 22bp)
ACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAATTC1082160.10228749842148924No Hit
CCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1078520.10194343978482347No Hit
TGTTGGAACAATGTAGGTAAGGGAAGTCGGCCTGTAGGCACCATCAATTC1074150.10153038037761758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)