FastQCFastQC Report
Wed 27 Sept 2023
SRR6359217.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6359217.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79139877
Total Bases4 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA35807604.524596367517731Illumina Single End Adapter 1 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT21236352.6833943651441357No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA14861481.8778750439554008No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATT10491271.325661650952528No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATT4474300.5653660543344033No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT4134330.522407938541527No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA4039680.5104481019094836No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC3913560.4945117617506532No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT3825290.4833580926591534No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT3774720.4769681408526829No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA3489930.44098248977566645No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG3489740.44095848165141827No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCTGTAGGCACCATCAATTC3316430.41905928158063727No Hit
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAAT3195170.403737043968365No Hit
GCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC3119020.3941148404868004No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT2539020.3208268822555789No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT2346400.29648769860989294Illumina Single End Adapter 1 (95% over 22bp)
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT2297940.29036436334112575No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCACCA2150230.2716999421164124No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG2134890.2697616019797453No Hit
GACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC2110170.26663801865651116No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA2097850.2650812813368411No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATTC2044730.2583691152312506No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT2008980.25385179711613653No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT1922150.24288008433472802No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC1914930.24196777561329794No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCACC1876620.23712697961357712No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGCTGTAGGCACCATC1875980.23704611014242544No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC1852880.234127227668044No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG1804110.2279647212491877No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT1792470.22649390774261627No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC1768100.2234145499114182No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT1715270.21673902778494336No Hit
AGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1665600.21046279867228No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA1637940.20696772121594273No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTGTAGGCACCATC1516900.1916732824843789No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGCTGTAGGCACCATCAA1473640.18620701166871917No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1449050.18309985495681272Illumina Single End Adapter 1 (95% over 22bp)
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCAC1403870.17739097572769794No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATTCGT1374700.1737050968628622No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1340700.16940890620792853No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG1339420.16924716726562514No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT1334750.16865707284331513No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1301150.16441142560785127No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATT1234890.1560389081726776No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA1231860.1556560417701938No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA1224590.15473741512133005No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA1217060.1537859352498109No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT1216910.15376698146750972No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTCG1207640.15259563772129695No Hit
ACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATTC1198650.15145967436871302No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA1189110.1502542138143581No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC1157880.14630803633925285No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATTC1147960.14505455953640162No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCTGTAGGCACCATCAA1122900.14188801430661815No Hit
TGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT1116970.14113870811297824No Hit
TGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1105950.13974623690658503No Hit
AACATGGGTCAGTCGGTCCTGAGAGATGGGCGCTGTAGGCACCATCAATT1097890.1387277870042684No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1079760.13643690651679938Illumina Single End Adapter 1 (95% over 22bp)
TGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCAA1038340.13120313543070075No Hit
GGGCCGCCCTCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATC1037060.13104139648839738No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA1027470.12982961800660872Illumina Single End Adapter 1 (95% over 22bp)
CGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTCGT1025380.12956552863987897No Hit
ACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCGTA1020370.12893247231101965No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT1001760.12658093972018683No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATTC979290.12374166313147038No Hit
TGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATC955730.12076465572469867No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTC953630.12049930277248219No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA944600.11935828507795129Illumina Single End Adapter 1 (95% over 22bp)
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTGTAGGCACCATC941660.11898679094484818No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATTC937250.11842954974519357No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT931620.11771815111615601No Hit
ACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGTAT925910.11699664380322451No Hit
GAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA900090.11373406607644841No Hit
TCACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCA887520.11214573911960969No Hit
GACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATTCG872020.11018718161515464No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCTGTAGGCACCATCAATTCG864600.1092496011839897No Hit
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG862990.10904616392062372No Hit
CCTCCCGACCCCTCCACCCGCGCGTCGCTGTAGGCACCATCAATTCGTAT844930.1067641285315619No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCA834020.1053855567655229No Hit
CCGTTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAA825480.1043064547598425No Hit
CACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATTCGTA823820.1040966995690428No Hit
TCCTCCCGACCCCTCCACCCGCGCGTCGCTGTAGGCACCATCAATTCGTA822780.10396528667842131No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGC819020.10349017853540511No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCTGTAGGCACCATCAATTCGT806270.10187910703980498No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGCTGTAGGCACCATCAATT806250.1018765798688315No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGCTGTAGGCACCATCAAT805330.10176033000405094No Hit
TTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATTCGTA793850.10030973386526744No Hit
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA792530.10014294058101707Illumina Single End Adapter 1 (95% over 22bp)
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCA792200.1001012422599545No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)