FastQCFastQC Report
Tue 26 Sept 2023
SRR6359218.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6359218.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94338696
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA55685795.902751719188486Illumina Single End Adapter 1 (95% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT39818704.220823658618304No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA28412113.011713242252151No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATT20445102.167201887123816No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA7706160.8168609835353247No Hit
TCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC6582050.6977041531292737No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTCTGTAGGCACCATCAATTC5925410.6280996294458002No Hit
TGGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAAT5838960.6189358394353893No Hit
GGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATT5673870.6014361275462192No Hit
ACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG4977350.5276042823403029No Hit
ACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT4815170.5104130334809801No Hit
GCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTC4608850.4885428986637679No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT3538460.375080444190155Illumina Single End Adapter 1 (95% over 22bp)
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCACC3526820.373846592070766No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA3504950.3715283493000582No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA3439250.36456408089422815No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCACCA3389830.3593255094388839No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC3307580.35060692380144837No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT3069140.3253320355413859No Hit
GACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC3067510.3251592538442549No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCA3035200.32173436020357965No Hit
GACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG3032140.3214099970175547No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCTGTAGGCACCAT2757830.2923328514101997No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATT2687330.2848597780066835No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT2648650.2807596577336621No Hit
TCACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTCG2545280.26980233010640725No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATT2458930.2606491402001147No Hit
TGCAGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATC2432710.25786979290025375No Hit
AGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT2370640.251290308273924No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCA2358290.24998119541529384No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCTGTAGGCACCATCAAT2312330.2451093875624484No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCAC2276210.2412806299548597No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC2167860.22979541714250534Illumina Single End Adapter 1 (95% over 22bp)
CCTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2119730.22469358703028924Illumina Single End Adapter 1 (95% over 22bp)
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT2032500.21544711620775422No Hit
TCACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCGT1890560.2004013284220083No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGAGCTGTAGGCACCATC1887360.2000621250902175No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCA1885220.1998352828620824No Hit
ACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATTC1849000.1959959251503752No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT1808600.19171348308651628No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAAT1778520.18852497176768268No Hit
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTGTAGGCACCATC1758460.18639859088151908No Hit
TCACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCA1722820.18262071377369896No Hit
TTTCCCGCTCCCCGTTCCTCCCGACCCCTCTGTAGGCACCATCAATTCGT1712830.18156176337226454No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCCCTGTAGGCACCA1700540.1802590105761055No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCGCCCCTGTAGGCACCATC1671470.1771775603088684No Hit
TGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATT1663300.17631153180238998No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA1631810.17297355901548608Illumina Single End Adapter 1 (95% over 22bp)
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAA1603820.17000658987272838Illumina Single End Adapter 1 (95% over 22bp)
TGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAAT1570500.16647463518045658No Hit
GGGCCGCCCTCTCGCCCGTCACGTTGAACGCACGTTCTGTAGGCACCATC1487150.15763944839771793No Hit
TCGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAAT1472900.156128933560837No Hit
TACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATTC1440900.15273690024292894No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA1437950.1524241971714343Illumina Single End Adapter 1 (95% over 22bp)
TACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTCG1431910.15178395088267915No Hit
TAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGCTGTAGGCACCATCAA1424740.15102392341738538No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTCTGTAGGCACCATCAATTC1421520.15068260006477088No Hit
TTGCAGGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATC1352910.1434098686290936No Hit
CGGGGCCCGGTGGGGGGCGGGGCGGACTGTCCCTGTAGGCACCATCAATT1311080.13897584507634067No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC1282730.1359707155587565No Hit
AAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCA1279620.1356410523206723No Hit
TCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATT1279260.13560289194584585No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGCTGTAGGCACCATCAATTC1276980.1353612095719449No Hit
TCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCA1272780.13491600519896949No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATTC1268120.1344220403470491No Hit
CGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTCGT1228630.13023605923066817No Hit
TCACCGCCCGTCCCCGCCCCTTGCCTCTCGGCCTGTAGGCACCATCAATT1202040.12741749154556897No Hit
TGAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATC1162380.12321349025218664No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAATTC1161170.12308522899235326No Hit
TTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA1157150.12265910480679106Illumina Single End Adapter 1 (95% over 22bp)
GTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCTGTAGGCACCATCAA1137190.1205433240247459No Hit
TGACACATTGATCATCGACACTTCGAACGCACTGTAGGCACCATCAATTC1086880.11521041164274733No Hit
TTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACCATCAATTC1067180.11312219113141016No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAATAA1059990.1123600436452927Illumina Single End Adapter 1 (95% over 22bp)
TACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCAT1057220.11206642076121127No Hit
AACATGGGTCAGTCGGTCCTGAGAGATGGGCGCTGTAGGCACCATCAATT1050050.1113063932959175No Hit
GAGGCGTCCAGTGCGGTAACGCGACCGATCCCGGACTGTAGGCACCATCA1046170.11089510925612117No Hit
CTGTAGGCACCATCAATTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1043060.11056544601803697Illumina Single End Adapter 1 (95% over 22bp)
TGGGCGAAGCTACCATCTGTGGGATTATGACTGCTGTAGGCACCATCAAT1038110.11004074086417305No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATTCG1037290.10995382001040166No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGCTGTAGGCACCATCAATT1029890.10916941230563543No Hit
TGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCATCAATT1024400.10858746658953183No Hit
TGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTGTAGGCACCAT1022990.1084380051214615No Hit
TGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCACC1016990.10780199887435375No Hit
TGTTGGAACAATGTAGGTAAGGGAAGTCGGCACTGTAGGCACCATCAATT1006180.10665612761914793No Hit
TGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGACTGTAGGCAC1005050.1065363464426093No Hit
ACCCGCGCGTCGTTCCCCTCTTCCTCCCCGCGTCCGCTGTAGGCACCATC1000890.10609538211128124No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTCTGTAGGCACCA976670.10352803689378959No Hit
TTGCAGGACACATTGATCATCGACACTTCGAACGCACCTGTAGGCACCAT969130.1027287890432575No Hit
GCCGGGCGCGGTGGCGCACGCCTGTAGTCCCAGCTACTGTAGGCACCATC966270.10242562606546947No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAA964730.10226238446204514No Hit
GACACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATT958440.10159563791299384No Hit
TCACATTGATCATCGACACTTCGAACGCACTCTGTAGGCACCATCAATTC954860.10121615418555287No Hit
TGCGGGTGTTGACGCGATGTGATTTCTGCCCACTGTAGGCACCATCAATT944930.10016356384658952No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)