FastQCFastQC Report
Tue 4 Jul 2023
SRR6398737.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398737.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61258311
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC12065751.9696511057903636No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC10571761.7257674636181202No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA7620371.2439732463404027No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA7460951.2179490224599892No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7031311.1478132330484918No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA5953240.9718256841916519No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA5436380.8874518267407013No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3753220.6126874768062084No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3032990.49511485878218225No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2938000.4796083914230022No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2904940.47421157269582576No Hit
ATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG2586140.4221696546612263No Hit
TATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGA2479740.40480058289560095No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT2435330.3975509543513206No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG2425490.39594464169931165No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1898840.30997263375413664No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1644220.26840766145184775No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1642540.2681334129502852No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC1637700.2673433160767361No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1459440.23824359114308588No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1438980.23490363617762822No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA1339270.21862666112358206No Hit
AGTGGGCCATTTTTGGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG1300630.21231894558764444No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1273910.20795708846755503No Hit
ATGATGCCCTGACGAGTAGGCCTGTAGGCACCATCAATAGATCGGAAGAG1254390.2047705820684478No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1229220.200661751839681No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1200540.19597993813443537No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1187170.19379737714283374No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1183370.19317705315120426No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1167140.1905276167343236No Hit
AAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1136270.18548830051811255No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA1087730.17756447774082443No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC1061490.17328097733546718No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1058410.17277818841593592No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA1001510.16348965285706293No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC991910.16192251856242004No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA989090.1614621728633687No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG964510.15744965609646014No Hit
TACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC936490.15287558287397118No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA908550.1483145691039376No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA895140.1461254783861083No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA890240.14532558692321765No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC878280.14337319878114171No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA867130.14155303759517626No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT866620.14146978358577336No Hit
AACTTTTCGAATCGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG847640.1383714284907398No Hit
ATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG845170.13796821789618063No Hit
ATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATC820050.1338675498251984No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA746280.12182510222980195No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA737280.12035591382857422No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA736050.12015512474707309No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT731060.11934054140017017No Hit
ATTTGAATGAAGAGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCA714290.11660295367921587No Hit
TAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAG688700.1124255613250584No Hit
CATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA685840.11195868589977938No Hit
AAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAG676840.11048949749855168No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG671260.10957860068979049No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC669680.10932067650379718No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGA659130.10759846121124692No Hit
AATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA658800.10754459096986857No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGAT655210.10695854804093438No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG646610.10555465690198348No Hit
CAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC637690.10409852795321112No Hit
AATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT632650.1032757824485236Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG631590.10310274470349012No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA628970.10267504763557715No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG613500.1001496760170224No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)