FastQCFastQC Report
Mon 10 Jul 2023
SRR6398738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46672983
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA8114471.7385796832398734No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA7434401.5928701193150647No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6657461.426405507443139No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6177471.3235644269833793No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA4996351.0705015361885055No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3338970.7153967424794768No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3333670.714261181891888No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT2545610.5454140353531721No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2326160.4983953993255584No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2240570.4800571671195732No Hit
TATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGA2236740.47923656390250435No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2227710.4773018257693107No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2138910.458275829509333No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA2099620.4498576831911515No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1853480.3971205354498126No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1291230.2766546976438168No Hit
AGTGGGCCATTTTTGGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG1269460.2719903289661173No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1265270.27109259333177826No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1139710.24419052024165672No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG989180.21193845698698968No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG949140.20335961813282857No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA921210.19737542809295047No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC908920.1947422130700324No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA873550.18716395307323724No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG835450.17900077224547656No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA834630.17882508173947229No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA803980.17225811343577505No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA781820.1675101846393662No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA749350.16055326911502527No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGA745820.15979694291234825No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG744240.15945841730321803No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG722440.15478762092407936No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT706010.15126738310255422No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG692440.1483599194848977No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA691270.14810923912876964No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC684510.14666086373780737No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC681630.14604380439964593No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA681520.1460202361610356No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA616090.13200141932218046No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA614030.13156005049002334No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG597220.1279583951169352No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA591490.1267307041420515No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG567480.12158640042355981No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG561270.12025586622564921No Hit
GAAAAGAAACCAACCGGGATTGCCTCTGTAGGCACCATCAATAGATCGGA552160.11830398755528439No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA548880.1176012255312672No Hit
TAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGATC545790.1169391722830315No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC544720.11670991759836734No Hit
AGACCGTAAGGTCGGGTCGAACGGCCTCTCTGTAGGCACCATCAATAGAT543550.11645923724223928No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA536180.11488016525534697No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA531950.11397385935242237No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG528330.11319825004542777No Hit
GGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG526130.11272688527322113No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA513750.1100743871459855No Hit
TGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG489580.1048958023531515No Hit
TAGCCCTGAAAATGGATGGCGCTCCTGTAGGCACCATCAATAGATCGGAA483280.10354598505092337No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG468040.10028071271981909No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)