FastQCFastQC Report
Wed 5 Jul 2023
SRR6398741.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398741.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133690719
Total Bases6.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA10359300.7748705428085849No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC8824090.6600375901935271No Hit
ATGATGCCCTGACGAGTAGGCCTGTAGGCACCATCAATAGATCGGAAGAG8131660.6082441668968809No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA8056240.6026027880065481No Hit
AACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAAGAG7988950.5975695291159292No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG6905850.516554182044604No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA6762210.5058099807212496No Hit
AAGAGATGGGGAAGCTCCGTTTCCTGTAGGCACCATCAATAGATCGGAAG6455500.48286822363488074No Hit
AGTGGGCCATTTTTGGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG6302750.47144259879401196No Hit
ATTGCTCTTCAACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAA6188400.46288927505880195No Hit
TAGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCG5938470.4441946340343939No Hit
ATGAATGGATTAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAAG5742300.42952121455790804No Hit
ATGGAAGTCGGAATCCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA5711690.4272316016192567No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA4954360.37058369025601545No Hit
ATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCGGAA4490700.33590215039534643No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGAT4304150.3219483021854344No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC4241850.31728829283953514No Hit
AAATCGATCGTCGAATTTGGGTCTGTAGGCACCATCAATAGATCGGAAGA4240920.3172187292971324No Hit
AGACCGTAAGGTCGGGTCGAACGGCCTCTCTGTAGGCACCATCAATAGAT4149590.3103872902351584No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG4149190.3103573704319744No Hit
AACAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAA4065640.3041078715419281No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGAT3935510.2943742115711114No Hit
TATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGA3869350.289425476124487No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG3763660.2815199161282093No Hit
TATGGATTCTTCACGGTAACGTCTGTAGGCACCATCAATAGATCGGAAGA3640110.2722784369197685No Hit
AGAACTGGCGATGCGGGATGAACCGAACGTCTGTAGGCACCATCAATAGA3623650.2710472370187492No Hit
AGGGGCGAAAGACTAATCGAACCCTGTAGGCACCATCAATAGATCGGAAG3462920.25902471210436084No Hit
TAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAG3400490.2543549788224267No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA3376640.252571010557584No Hit
TGATGCGGGATGAACCGAACGTCTGTAGGCACCATCAATAGATCGGAAGA3327710.24891107063310805No Hit
TAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGATC3314410.2479162371772419No Hit
TAAGGTAGCCAAATGCCTCGTCCTGTAGGCACCATCAATAGATCGGAAGA3245310.24274759117721553No Hit
AGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGAGC3177880.23770386035548213No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG3174350.23743981809238382No Hit
TAGCCCTGAAAATGGATGGCGCTCCTGTAGGCACCATCAATAGATCGGAA3173350.23736501858442394No Hit
AGGGATAACTGGCTTGTGGCAGTCCTGTAGGCACCATCAATAGATCGGAA3163240.23660879555894976No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAA3128210.23398856879511581No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT3041580.22750868742055314No Hit
TAGACGTTCTGGGCCGCACGCGCGCTCTGTAGGCACCATCAATAGATCGG2866860.21443971738980624No Hit
ATTTGAATGAAGAGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCA2772140.20735470799584824No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG2750920.20576746243694002No Hit
ACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGAGC2669640.19968775842996253No Hit
TAAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAA2667580.1995336714435652No Hit
TAAGGATTGGCTCTAAGGGTCGGCTGTAGGCACCATCAATAGATCGGAAG2664540.19930628093936723No Hit
ACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGA2641640.1975933722070864No Hit
TGTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGA2622100.19613178982155074No Hit
AGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGATC2619080.19590589530751198No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA2590190.19374493752255156No Hit
AAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAATAGATCGGAA2554800.1910977829358521No Hit
TGAAGCCAGAGGAAACTCTGGTGGAGGCTCGTCTGTAGGCACCATCAATA2481750.18563367887938428No Hit
ATTTTGCGTGGGGATAAATCCTGTAGGCACCATCAATAGATCGGAAGAGC2471920.18489839971613886No Hit
ATCATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCG2384550.17836316670568583No Hit
ATGGCTGAACGCCTCTAAGTCAGAATCCCTGTAGGCACCATCAATAGATC2380050.17802656891986646No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT2370930.17734439740727254No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT2348280.1756501885519817No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2322940.1737547690202788No Hit
GGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG2311550.172902802624616No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCCTGTAGGCACCATCAATAGA2282970.17076503268712317No Hit
TGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG2277240.17033643150651318No Hit
AAGGAGTGTGTAACAACTCACCCTGTAGGCACCATCAATAGATCGGAAGA2260020.16904838397944438No Hit
AAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAA2228300.16667574358695758No Hit
AGGATAGCAGAAGCTCGTATCCTGTAGGCACCATCAATAGATCGGAAGAG2212800.16551635121357977No Hit
ATGAAAGTGTGGCCTATCGATCCTTTCTGTAGGCACCATCAATAGATCGG2207530.16512215780663128No Hit
AACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGAG2198070.164414554461331No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA2188820.16372265901270228No Hit
TATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCGGAAGAG2166190.16202994614757066No Hit
GTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGAA2150590.16086307382339682No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2147590.16063867529951723No Hit
ACTGATGAATGTTACCGCAATCTGTAGGCACCATCAATAGATCGGAAGAG2121660.15869912405811804No Hit
ACAGCCAAGGGAACGGGCTTGGCCTGTAGGCACCATCAATAGATCGGAAG2101320.15717770206621448No Hit
TTAACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAG2098530.1569690114390065No Hit
TTTAAGGTAGCCAAATGCCTCGTCCTGTAGGCACCATCAATAGATCGGAA2090080.15633695559674565No Hit
AGAAAAGTTACCACAGGGATCTGTAGGCACCATCAATAGATCGGAAGAGC2071150.15492100091106548No Hit
TGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCGGAAG2056700.1538401480210455No Hit
TAAGGTGCCGGAATACACGCTCATCCTGTAGGCACCATCAATAGATCGGA1976210.14781953562535632No Hit
AGGAGTACCCGCTGAACTTAAGCCTGTAGGCACCATCAATAGATCGGAAG1873100.1401069583596151No Hit
ACGTCCCTGCCCTTTGTACACCTGTAGGCACCATCAATAGATCGGAAGAG1858830.13903956938102785No Hit
AAGATTCCGGAACCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCA1834970.1372548531211056No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGCCTGTAGGCACCATCAATAGA1830160.13689506748781866No Hit
ATTTTTGGTAAGCAGAACTGGCCTGTAGGCACCATCAATAGATCGGAAGA1819200.13607526488057858No Hit
TAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGAG1813980.1356848114490281No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA1811500.13549930866928764No Hit
TAGTCCCTCGGAATTTGAGGCTCTGTAGGCACCATCAATAGATCGGAAGA1798750.13454561494279943No Hit
TAGGATAGCAGAAGCTCGTATCCTGTAGGCACCATCAATAGATCGGAAGA1791330.13399060259373727No Hit
AAGGCTGAAACTTAAAGGAATTGACCTGTAGGCACCATCAATAGATCGGA1783280.13338846655466038No Hit
AAAATGAGAACTTTGAAGACTCTGTAGGCACCATCAATAGATCGGAAGAG1781590.13326205538620822No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG1780200.133158084070144No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1777220.13293518153642364No Hit
TAGCCGGACGGTGGCCATGGAAGTCCTGTAGGCACCATCAATAGATCGGA1740140.130161615781272No Hit
AAATCGATCGTCGAATTTGGGTATCTGTAGGCACCATCAATAGATCGGAA1734250.1297210466793884No Hit
AGGTCGTACTGATAACCGCAGCCTGTAGGCACCATCAATAGATCGGAAGA1724420.128985767516143No Hit
TAAGGTGCCGGAATACACGCTCCTGTAGGCACCATCAATAGATCGGAAGA1693330.12666025081367094No Hit
TTGACCTCAAATCAGGTAGGAGTCTGTAGGCACCATCAATAGATCGGAAG1679670.12563848953493922No Hit
TTTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGAT1677050.1254425148240844No Hit
AGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCGG1676480.12539987910454728No Hit
AACGCAGATGTCCTAAGGGGGGCTCCTGTAGGCACCATCAATAGATCGGA1642160.12283275999136485No Hit
AAAAGTCTTTGGGTTCTGGGGGGAGTCTGTAGGCACCATCAATAGATCGG1613620.12069798203419041No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1609860.12041673588426136No Hit
AGTACGAGAGGAACAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1588360.11880854646312433No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1561140.11677250385645693No Hit
AAAATCGATCGTCGAATTTGGGTCTGTAGGCACCATCAATAGATCGGAAG1558410.11656830119972653No Hit
AGCTGGTTCCTGCCGAAGTTTCCCTCCTGTAGGCACCATCAATAGATCGG1555760.11637008250363289No Hit
ATCTAGACAGCCGGACGGTGGCCCTGTAGGCACCATCAATAGATCGGAAG1523210.11393535851953941No Hit
GATGCGGGATGAACCGAACGTCTGTAGGCACCATCAATAGATCGGAAGAG1513700.11322401519884115No Hit
AAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAAG1512340.11312228786801573No Hit
TAGCGGTTCTGACGTGCAAATCCTGTAGGCACCATCAATAGATCGGAAGA1511650.11307067620752342No Hit
TAGTGACGCGCATGAATGGATTCTGTAGGCACCATCAATAGATCGGAAGA1482070.11085810676207074No Hit
ACCACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGA1481440.11081098307205604No Hit
TTGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG1478340.11057910459738046No Hit
AAAAAGCCCCCTTGATTTTGATTTTCCTGTAGGCACCATCAATAGATCGG1474270.11027467059998383No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAAT1460210.10922298951806819No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA1441430.107818254758582No Hit
TAACAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGA1420500.10625270105698212No Hit
TACGGACCAAGGAGTCTAACGTCTCTGTAGGCACCATCAATAGATCGGAA1419800.10620034140141023No Hit
ATCTACTATCTAGCGAAACCACCTGTAGGCACCATCAATAGATCGGAAGA1406080.10517409215220093No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1402000.10486891015972469No Hit
TAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATCGGAAGAGC1359700.10170489097302259No Hit
TACCGTCAGGGTTGATATGATGCCCTGACGAGTCTGTAGGCACCATCAAT1342130.10039066361816784No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)