FastQCFastQC Report
Wed 5 Jul 2023
SRR6398742.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398742.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201974722
Total Bases10 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA22042551.0913519168007568No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA21962531.087390034877731No Hit
ATGGAAGTCGGAATCCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCA15887240.7865954631689009No Hit
AACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAAGAG15799180.7822355116300148No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA14371750.7115618161365758No Hit
ATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCGGAA13902110.6883094014111331No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC11924040.5903728883458991No Hit
TAGTCCCTCGGAATTTGAGGCTCTGTAGGCACCATCAATAGATCGGAAGA11865120.5874556916087746No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA11771560.5828234287655066No Hit
AGTGGGCCATTTTTGGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG11167390.5529102795348816No Hit
ATGTAAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGA11146360.5518690601292175No Hit
ATGATGCCCTGACGAGTAGGCCTGTAGGCACCATCAATAGATCGGAAGAG10471310.5184465608523031No Hit
ATTGCTCTTCAACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAA9362680.46355701878376643No Hit
AGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAGC8930250.44214691381033316No Hit
TATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGA7407090.36673351628626083No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA7192860.35612674342485295No Hit
AGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGAGC7044080.34876047508559016No Hit
ATGAATGGATTAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAAG6658850.3296872962152165No Hit
AACAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAA6469970.3203356309112781No Hit
TAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAAGAG6372510.3155102745976301No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6252450.30956596637870354No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA6238110.30885597654146046No Hit
ATTTGAATGAAGAGCTTTTCTGTAGGCACCATCAATAGATCGGAAGAGCA6236400.3087713124813708No Hit
GGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG6169820.30547486036396176No Hit
AAGAGATGGGGAAGCTCCGTTTCCTGTAGGCACCATCAATAGATCGGAAG6020590.2980863120088859No Hit
TGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG5757780.28507428766259174No Hit
TAAGGTAGCCAAATGCCTCGTCCTGTAGGCACCATCAATAGATCGGAAGA5703100.28236701818557275No Hit
TAGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCG5627460.2786219950831273No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT5510040.27280839629030407No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC5503830.27250093207208376No Hit
TGATGCGGGATGAACCGAACGTCTGTAGGCACCATCAATAGATCGGAAGA5313550.2630799511633936No Hit
AGACCGTAAGGTCGGGTCGAACGGCCTCTCTGTAGGCACCATCAATAGAT5244860.2596790305273944No Hit
AACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGAG5192980.2571103922598789No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5009320.24801717513937213No Hit
AGATGTCCTAAGGGGGGCTCCTGTAGGCACCATCAATAGATCGGAAGAGC4883010.24176342225637523No Hit
TAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGATC4867060.24097371947366758No Hit
AGAAAAGTTACCACAGGGATCTGTAGGCACCATCAATAGATCGGAAGAGC4754690.23541015196941328No Hit
ACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGAGC4747720.235065059280042No Hit
AAATCGATCGTCGAATTTGGGTCTGTAGGCACCATCAATAGATCGGAAGA4697940.2326003944196542No Hit
ATGATGCCCTGACGAGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA4664890.23096405103604994No Hit
AGGGATAACTGGCTTGTGGCAGTCCTGTAGGCACCATCAATAGATCGGAA4663370.23088879409374802No Hit
TAGCCCTGAAAATGGATGGCGCTCCTGTAGGCACCATCAATAGATCGGAA4509720.22328140647224162No Hit
AGTACGAGAGGAACAGTTCCTGTAGGCACCATCAATAGATCGGAAGAGCA4494510.22252834193775992No Hit
TTGACCTATTCTCAAACTTTCTGTAGGCACCATCAATAGATCGGAAGAGC4308240.21330590072553735No Hit
AGGATAGCAGAAGCTCGTATCCTGTAGGCACCATCAATAGATCGGAAGAG4171620.20654168792468988No Hit
ACAGCCAAGGGAACGGGCTTGGCCTGTAGGCACCATCAATAGATCGGAAG4039230.1999869072724853No Hit
ACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGA4017010.19888676960278226No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA3941430.1951447171691119No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG3928090.19448423847812005No Hit
ATGGAGAACAGAAATCTCCAGTCTGTAGGCACCATCAATAGATCGGAAGA3897580.19297365340599404No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGAT3894690.1928305661933279No Hit
ATGGCTGAACGCCTCTAAGTCAGAATCCCTGTAGGCACCATCAATAGATC3798410.18806363303225637No Hit
TTAACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAG3783100.18730561738314955No Hit
ACTGATGAATGTTACCGCAATCTGTAGGCACCATCAATAGATCGGAAGAG3769840.1866490995838578No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG3768500.18658275464788115No Hit
TAAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAA3751920.1857618598430352No Hit
TACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGAGC3738470.18509593492595575No Hit
AGGATAGCAGAAGCTCGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC3712620.1838160717953606No Hit
TATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCGGAAGAG3688870.18264018207189311No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG3682940.18234658097462314No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG3615840.17902438306116344No Hit
TAGACGTTCTGGGCCGCACGCGCGCTCTGTAGGCACCATCAATAGATCGG3540920.17531500798401892No Hit
TAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGAG3431100.1698776939027053No Hit
ACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3416100.16913502670893638No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA3357230.1662203055291246No Hit
AACGCAGATGTCCTAAGGGGGGCTCCTGTAGGCACCATCAATAGATCGGA3356360.16617723083188599No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGAT3262660.16153803642814274No Hit
ATCATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCG3217600.15930706417806084No Hit
AAGGAGTGTGTAACAACTCACCCTGTAGGCACCATCAATAGATCGGAAGA3206330.15874907355980913No Hit
AAGATTCCGGAACCTGGATCTGTAGGCACCATCAATAGATCGGAAGAGCA3119500.15445002073081204No Hit
AATGACCCACTCGGCACCTTCTGTAGGCACCATCAATAGATCGGAAGAGC3099450.15345732224847425No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT3040280.15052774772478708No Hit
AGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGATC3010070.14903201599653643No Hit
CGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAAG2965950.14684758422393074No Hit
TTGGATTTGAGTAAGAGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2963360.14671935035514No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA2940690.14559693266962387No Hit
TAGGATAGCAGAAGCTCGTATCCTGTAGGCACCATCAATAGATCGGAAGA2937160.14542215832335692No Hit
AACAGGTCTGTGATGCCCTTCTGTAGGCACCATCAATAGATCGGAAGAGC2892120.14319217629620007No Hit
TAAGGTGCCGGAATACACGCTCCTGTAGGCACCATCAATAGATCGGAAGA2842310.1407260261014247No Hit
AAAAAGGTGTTAGTTCATCTAGACCTGTAGGCACCATCAATAGATCGGAA2811610.13920603391151096No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG2746070.13596107338620325No Hit
AAGAAGTCCTTGTTACTTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2743880.13585264397591298No Hit
AAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAAG2714720.13440889895122618No Hit
GATGCGGGATGAACCGAACGTCTGTAGGCACCATCAATAGATCGGAAGAG2686150.13299436550282762No Hit
AGCGGTTCTGACGTGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2658180.13160953874217982No Hit
AAGGGGAATCTGACTGTCTCTGTAGGCACCATCAATAGATCGGAAGAGCA2642380.13082726263140987No Hit
AGACCGTAAGGTCGGGTCGAACCTGTAGGCACCATCAATAGATCGGAAGA2641350.1307762661507711No Hit
ACGGGGAAACTCACCAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG2637140.13056782422505328No Hit
TAGTGACGCGCATGAATGGATTCTGTAGGCACCATCAATAGATCGGAAGA2577900.1276347839211285No Hit
TAACGATCAACTTAGAACTGGTCTGTAGGCACCATCAATAGATCGGAAGA2568360.12716244758589146No Hit
AAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAA2560800.12678814332023192No Hit
ATCTACTATCTAGCGAAACCCTGTAGGCACCATCAATAGATCGGAAGAGC2552360.12637026924587128No Hit
AACTGGCTTGTGGCAGTCAAGCCTGTAGGCACCATCAATAGATCGGAAGA2527000.12511466657693926No Hit
TCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAG2515430.12454182261481216No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2515280.12453439594287447No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2487510.12315947141147691No Hit
AACTGGCTTGTGGCAGTCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2469370.12226133921847902No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA2450420.12132310299701762No Hit
TGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCGGAAG2427390.12018286129885104No Hit
AAAATGAGAACTTTGAAGACTCTGTAGGCACCATCAATAGATCGGAAGAG2398190.1187371358283142No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2381500.11791079479738065No Hit
TAGGCGTGGAGGTCAGTGACCTGTAGGCACCATCAATAGATCGGAAGAGC2359230.11680818157036503No Hit
ATTTTTGGTAAGCAGAACTGGCCTGTAGGCACCATCAATAGATCGGAAGA2340860.11589866181372933No Hit
TATGGATTCTTCACGGTAACGTCTGTAGGCACCATCAATAGATCGGAAGA2327940.11525897780416304No Hit
AGACAGCCGGACGGTGGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA2291650.11346221830670475No Hit
TAAGGTGCCGGAATACACGCTCATCCTGTAGGCACCATCAATAGATCGGA2266650.1122244396504232No Hit
ATGAAAGTGTGGCCTATCGATCCTTTCTGTAGGCACCATCAATAGATCGG2250910.11144513420842832No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2242460.11102676502260514No Hit
AGGGGCGAAAGACTAATCGAACCCTGTAGGCACCATCAATAGATCGGAAG2160740.106980714150952No Hit
TAGCCGGACGGTGGCCATGGAAGTCCTGTAGGCACCATCAATAGATCGGA2160340.1069609096924515No Hit
TAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAAGA2156630.10677722333985933No Hit
ATCTGTTAAACGATAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2139710.10593949474528798No Hit
AGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCGG2135920.10575184750099569No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT2127230.10532159564007222No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA2116140.10477251702814573No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2106750.10430760736484639No Hit
ATCTGTAAAACGATAACGCCTGTAGGCACCATCAATAGATCGGAAGAGCA2098540.10390112085412351No Hit
AAGCCGATGGAAGTTTGAGGCCTGTAGGCACCATCAATAGATCGGAAGAG2080910.10302823934571377No Hit
TATGCTAGAACGCGGTGATTTCCTGTAGGCACCATCAATAGATCGGAAGA2076360.10280296363027051No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)