FastQCFastQC Report
Mon 10 Jul 2023
SRR6398743.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398743.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118187419
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA45295813.832540754612807No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG19309441.633798264094421No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA12007551.015975312905344No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC9862080.8344441467158191No Hit
TTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATCG8263470.6991835569232627No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT7169120.6065890989632323No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA6413180.5426279763330816No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA6303090.5333131100866159No Hit
ACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGG6110570.5170237282193293No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA6055870.512395486020386No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6046320.5115874473915029No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG5499660.465333793269485No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC5462350.46217694287748173No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGAT5084610.4302158421785994No Hit
CAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGAT4803550.4064349691907563No Hit
ACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCG4742000.4012271390747606No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4242490.3589629112723073No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT4062870.3437650161393236No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCG4057750.3433318059005925No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGA3698310.31291909335967477No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3569730.30203976279404154No Hit
TGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATCGG3520430.29786842201875985No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATC3381300.2860964414494913No Hit
TCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGA3327720.2815629639902704No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA3267690.276483743163898No Hit
ACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATCGGA3249720.27496327675960164No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3134840.26524312202807304No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG3036950.2569605145535837No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2869910.2428270305149823No Hit
TTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATC2830150.2394628822548363No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATC2787490.23585336100790896No Hit
AAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATCGGAA2627340.22230284934134995No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2618920.22159042156593675No Hit
TTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAAT2603620.2202958675322286No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA2391340.2023345648998393No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGCTGTAGGCACCATCAATAG2357450.19946708540948846No Hit
TTGACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATAG2332460.1973526471544319No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA2319930.1962924666287873No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2259560.19118447793499915No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2253830.19069965475766926No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC2250740.19043820560968508No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA2222280.1880301658842385No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA2189690.1852726811810655No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGATC2142850.1813094843876741No Hit
TCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAAT2139920.1810615730596503No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT2126870.17995739461913454No Hit
TATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGAAGA2105400.1781407884032056No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1968220.16653380001470378No Hit
TTTGCTGGCTCCGGTGCGCTTGTGCTGTAGGCACCATCAATAGATCGGAA1898460.1606313105119928No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA1854890.15694479291404106No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGCTGTAGGCACCATCAATAGATC1843210.15595653205693577No Hit
CTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGA1837460.15547001665211083No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1827150.15459767337841604No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1802560.15251707967326034No Hit
TGACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATAGA1745260.14766884789996132No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGCTGTAGGCACCATCAATAG1716490.14523457864834158No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGCTGTAGGCACCATCAATAG1708190.14453230423789862No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA1699880.14382918371370815No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1646300.1392957062544872No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGCTGTAGGCACCATCAATAGATCG1642940.13901141203531994No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1632700.13814499155785778No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG1610930.13630300192950318No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA1599830.13536381566975417No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGATC1596910.13511675045547783No Hit
ATGATGCCCTGACGAGTAGGCCTGTAGGCACCATCAATAGATCGGAAGAG1594040.13487391580993913No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1578590.1335666700700182No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA1552800.1313845427151599No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT1539730.1302786720471491No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC1537490.13008914256770426No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG1531300.12956539815798837No Hit
AGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGATCGGAAGAG1525770.1290974972556089No Hit
AGTGGGCCATTTTTGGTAAGCCTGTAGGCACCATCAATAGATCGGAAGAG1511330.12787570900418765No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC1505350.12736973298316973No Hit
AAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGAAGA1421090.12024037854655241No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA1359380.11501901061059638No Hit
TTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACCATCAATAGATCG1352600.11444534548977672No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGCTGTAGGCACCATCAATAGAT1337220.11314402254608844No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC1329470.11248828439175916No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1328110.11237321292209622No Hit
TCTTGTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAA1283110.10856570105824885No Hit
CAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATAG1242860.10516009322447425No Hit
ACGATCTGCTGAGATTAAGCCTTTGTTGCTGTAGGCACCATCAATAGATC1239800.10490118241773264No Hit
TAGCCTGTGGGAATACTGCCAGCTGGCTGTAGGCACCATCAATAGATCGG1215220.10282143482632446No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGCTGTAGGCACCATCAAT1206720.10210223814093106No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA1205000.10195670657635733No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1182350.10004025893822083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)