FastQCFastQC Report
Tue 4 Jul 2023
SRR6398744.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398744.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91528538
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT49820215.4431340310494205No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA31205383.4093606957864875No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG15353841.67749210634174No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC14716871.6078996039464764No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA14699741.6060280565171927No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT9987991.0912432579224636No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG7408580.8094284211116756No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6628670.7242189315861246No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA6182150.6754341471072115No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC5711320.6239933604096244No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT5064050.553275525934873No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4914670.5369549331160518No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4662450.5093985003890262No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4413960.48224959083253355No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG4023560.4395962273537025No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA3929710.42934259476536163No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG3905240.4266691116600158No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3714260.40580348830656515No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3701920.404455274922014No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCA3320290.3627600825438728No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA3134530.34246477311808476No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA2954190.3227616287282989No Hit
ACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATCGGAAGAG2949490.32224812768231914No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2867270.31326513704392395No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC2757360.30125686045591593No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2430850.265583833536159No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG2359880.2578299677418643No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT2249100.24572663883257917No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC2215320.24203598663402665No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA2185600.23878891193476726No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA2184670.2386873042809883No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2159610.23594936040604078No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA2118270.2314327363122527No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2070180.22617863731200427No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGATC1963770.21455275511993865No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1936460.21156898627617107No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1928060.21065123972590932No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1922330.21002520547198075No Hit
AACTTTTCGAATCGCATGGCCCTGTAGGCACCATCAATAGATCGGAAGAG1896780.20723372638160134No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA1854260.20258818074860976No Hit
AGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCAATAGATCGGAA1840900.20112852671152684No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1790180.19558708563661314No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATCA1751100.19131737906706212No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1703900.18616051749892476No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1667340.18216613489445227No Hit
TTAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATC1663890.18178920327559478No Hit
ATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATC1627930.17786037399614096No Hit
TATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1587040.17339291489611688No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1578100.1724161703533383No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1576240.17221295504578038No Hit
AATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATA1574760.17205125684406758No Hit
GGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1547110.1690303411161227No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCTGTAGGCACCATCA1537710.16800333902416315No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA1511170.16510369694750288No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1477940.16147313529688415No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1469260.160524797194947No Hit
ATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1458820.1593841693396217No Hit
GAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAAT1451700.1586062698827332No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT1425060.15569570225190313No Hit
TGTACAACGGGGTATTGTAAGCCTGTAGGCACCATCAATAGATCGGAAGA1370620.1497478305618735No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATCA1315210.143693980996397No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1313250.14347984013466925No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA1258930.1375450791096434No Hit
TGGGAATACTGCCAGCTGGGACTGAGGACTGTAGGCACCATCAATAGATC1253780.13698241306989958No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1249120.13647328224558772No Hit
ATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1246710.13620997639009594No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATAGGCTGTAGGCACC1226840.13403906877656016No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1203180.13145408265998962No Hit
TAGCCTGTGGGAATACTGCCAGCTGGGACTGAGGCTGTAGGCACCATCAA1192870.13032765802508503No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1178420.12874891544755146No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCATCTGTAGGCACCA1146900.125305180773236No Hit
ATTCAATGAAGCGGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1108410.1210999349732867No Hit
CTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1102360.12043893894601486No Hit
GTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1095800.1197222225924771No Hit
TGTACAACGGGGTATTGTAAGCAGTCTGTAGGCACCATCAATAGATCGGA1080240.11802220636365895No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA1065690.1164325382319556No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1058130.11560656633672003No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1036810.11327723818772238No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1016710.11108120179959609No Hit
AGCGGCAAAAGCTCAAATTTGAAATCTGGTCTGTAGGCACCATCAATAGA998160.10905451150110144No Hit
GGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGATC968780.10584458368601932No Hit
TTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGAT946330.10339179677490314No Hit
AAATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGA933060.101941975736573No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)