FastQCFastQC Report
Wed 5 Jul 2023
SRR6398745.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398745.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152346672
Total Bases7.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA110150367.230243926824999No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG30266831.9867076584383807No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG25962481.7041711288580035No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG16227011.0651371498289113No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA14552710.9552364885266414No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA9707570.6372026295395543No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG8294250.5444326345376288No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA8170080.5362821447126853No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG7931590.5206277167643019No Hit
TTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAG7754710.5090173548392314No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA7669640.503433379890307No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA7326820.4809307550873182No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC7020910.4608508940713848No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC6450680.42342112993449577No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA6398260.4199802933666973No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA6392690.4196146798664562No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG5921750.388702288160256No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA5776870.3791923987679888No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA5720360.3754830955545914No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA5520820.3623853365172296No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA5192510.3408351447283338No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG5070540.3328290623900206No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC4814850.31604563045525536No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG4663390.30610383139843056No Hit
TAGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCG4380090.28750808550645596No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGAT4294800.28190966980886856No Hit
TGTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGA3910780.2567026866198955No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3666630.24067673759227246No Hit
TTTAAGGTAGCCAAATGCCTCGTCCTGTAGGCACCATCAATAGATCGGAA3498810.2296610719530519No Hit
AGACCGTAAGGTCGGGTCGAACGGCCTCTCTGTAGGCACCATCAATAGAT3382350.22201666472898077No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGAT3326990.21838284724723095No Hit
ATTGCTCTTCAACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAA3276100.21504243952240715No Hit
TAAGGTGCCGGAATACACGCTCATCCTGTAGGCACCATCAATAGATCGGA3091540.20292796418946388No Hit
AGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGAAGA2938330.19287129554100138No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT2932340.19247811333876727No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAA2872880.18857517281375205No Hit
TAAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAA2793480.18336337534173375No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG2741060.17992253877393527No Hit
AAGAGATGGGGAAGCTCCGTTTCCTGTAGGCACCATCAATAGATCGGAAG2723680.17878171962955647No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2719570.17851194018862454No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA2716830.17833208722800323No Hit
ATGAATGGATTAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAAG2694640.17687554080603743No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT2690770.17662151490910152No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2671520.175357949401087No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA2667800.1751137694691486No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2648280.1738324812241386No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA2624590.17227747515219763No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA2579380.1693099013019464No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2411970.1583211479670524No Hit
CTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA2387790.15673397840945288No Hit
AACAACGGATCTCTTGGTTCTCGCCTGTAGGCACCATCAATAGATCGGAA2176230.14284722937695676No Hit
TTCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGG2161460.14187773002353474No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA2160520.14181602864288365No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGA2158860.14170706663024446No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG2148250.14101062870608688No Hit
ACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGA2073480.13610274335365857No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA2071320.13596096145769432No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG2055640.1349317299166207No Hit
AGCTGGTTCCTGCCGAAGTTTCCCTCCTGTAGGCACCATCAATAGATCGG2024970.132918558273462No Hit
AACAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAA1990360.13064676594970187No Hit
TCGATCAACTTAGAACTGGTACGGACCTGTAGGCACCATCAATAGATCGG1965760.1290320276901093No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1962810.12883839037849149No Hit
ACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGC1894920.1243821066206159No Hit
ATCTAGACAGCCGGACGGTGGCCCTGTAGGCACCATCAATAGATCGGAAG1840080.12078242181752419No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACCTGTAGGCACCATCAATAGATCGG1839310.12073187919720359No Hit
ATCATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCG1838410.1206728034072185No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG1822570.11963306950348086No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1814000.11907053670328946No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1778010.1167081615015522No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1765250.11587059807909687No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1746440.1146359140684084No Hit
TTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAA1702580.11175695390313481No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1688700.11084587394203137No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1662030.10909526136547308No Hit
TGGCGCCAGTGAAATACCACTACCTTTCTGTAGGCACCATCAATAGATCG1647370.10813298238638255No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA1628180.10687335526436705No Hit
AGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGATC1628060.10686547849236903No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1615000.10600822313991867No Hit
ACGATCAACTTAGAACTGGTACGGACCTGTAGGCACCATCAATAGATCGG1600590.10506235410249067No Hit
AGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGATCGGAAG1592030.10450047769996577No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1585870.10409613673740115No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATTAATAGATCGGA1559260.10234946254684187No Hit
ATGATGCCCTGACGAGTAGGCCTGTAGGCACCATCAATAGATCGGAAGAG1556860.1021919271068816No Hit
TAGACGTTCTGGGCCGCACGCGCGCTCTGTAGGCACCATCAATAGATCGG1553160.10194905997027622No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1530670.10047282161831537No Hit
TAAGGATTGGCTCTAAGGGTCGGCTGTAGGCACCATCAATAGATCGGAAG1529820.10041702781666277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)