FastQCFastQC Report
Fri 7 Jul 2023
SRR6398746.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398746.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124315263
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA33572372.7005831134347518No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA27044072.175442447481288No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG17090471.3747684385303516No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG13216121.0631132236755192No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT11960750.9621304505465271No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT6073620.4885659132619942No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG5730870.46099488202023914No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA5510590.4432754166316649No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC5263800.42342346973114636No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG5177980.4165200535351801No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA4936280.3970775495202065No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA4662820.3750802506044652No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4535860.3648675062530335No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG4152260.33401047464300504No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA4013610.32285737914579327No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3916850.31507394228816454No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG3783220.30432465883131343No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT3645360.29323511144403885No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT3539860.28474862334482615No Hit
TTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATCG3263540.2625212641829829No Hit
ACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGG3161380.2543034478397073No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2905480.23371868665877338No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA2803230.22549363065740366No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA2799530.22519600026909004No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA2739350.2203550822234917No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGAT2698670.2170827567649517No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2436710.1960105252723473No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2404190.19339459548100701No Hit
TGGCGCCAGTGAAATACCACTACCTTTCTGTAGGCACCATCAATAGATCG2402770.193280369764411No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2288510.1840892216107044No Hit
ACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATCGGA2224470.17893780267351403No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2164320.17409929784728043No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2163980.17407194802781378No Hit
TTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2089520.16808233756461585No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCCTGTAGGCACCATC2009710.16166236964804556No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATC1989040.15999966150576378No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC1957000.1574223432242588No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC1954080.1571874565394275No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1947070.1566235676145414No Hit
ACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCG1908210.1534976441307935No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGACTGTAGGCACCATCAATAGAT1874470.15078357675195525No Hit
TTTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCG1851500.14893585512504606No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1838400.14788208266912486No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGCTGTAGGCACCATCAATAGATC1785330.14361309761296165No Hit
TCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAAT1731570.13928860851141023No Hit
ATTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1730520.13920414583364554No Hit
TGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGATC1690790.13600823898832118No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA1687590.13575082892275264No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA1672590.1345442192404001No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGATC1635900.13159285195736584No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1628520.1309991999936484No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG1615910.1299848434540174No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1595140.1283140912471866No Hit
TCGATCAACTTAGAACTGGTACGGACCTGTAGGCACCATCAATAGATCGG1493580.12014453929120514No Hit
TTCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGG1488110.11970452896037391No Hit
TGGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATC1486120.11954445207584849No Hit
TACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGA1442190.1160106945194654No Hit
TTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATC1407860.11324916715978793No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA1401420.11273112940283125No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCG1354120.10892628687114632No Hit
TTGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1334300.10733195327753119No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1318200.10603685888513946No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG1296910.10432427754265379No Hit
ATTGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1288320.10363329239789325No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA1262750.10157642509270966No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGA1260770.10141715261463911No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1250700.10060711531455313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)