FastQCFastQC Report
Wed 5 Jul 2023
SRR6398747.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398747.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100476127
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1107484811.022367532140247No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG72299637.195702318422365No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG51188495.0945922706594775No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG32821903.2666366608657196No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG16398101.632039419672297No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG10787771.073664991087883No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG10317741.0268847245674588No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA9417570.937294288821463No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA5073120.5049079967025402No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA5026150.5002332544127621No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA4070750.405145990549576No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA3768840.375098056874744No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA3175760.3160711001529746No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG3059560.3045061639368325No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA2944910.2930954932209917No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG2820970.2807602247646349No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG2616740.2604340033926666No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA2545720.2533656576949866No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2428910.24174001054001615No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG2395820.23844669092390475No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT2391940.23806052954250517No Hit
GAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAA2328030.23169981462362693No Hit
TTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATC2134580.21244648492472246No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGACGTCCTGTAGGCACCATCAA2125060.21149899617448434No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2086510.20766226389279516No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT1855710.18469163326727353No Hit
TGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1799790.17912613212091663No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGA1743740.1735476925777603No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1729160.17209660161363505No Hit
TAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1681800.16738304413345867No Hit
TCGTGAATCATCGAATCTTTGAACGCCTGTAGGCACCATCAATAGATCGG1679020.16710636149420846No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1611390.16037540937460698No Hit
GAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGA1604960.1597354563636793No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC1602870.1595274467535955No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1539670.15323739538646827No Hit
TTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCG1538720.1531428455637029No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG1531620.15243621004619334No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATTAATAGATCGG1525980.1518748826773548No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA1486770.14797246315037602No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA1458280.1451369637287074No Hit
TCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGA1432870.14260800478505703No Hit
AACAACGGATCTCTTGGTTCTCGCCTGTAGGCACCATCAATAGATCGGAA1427100.14203373901941901No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATTAATAGATCGGA1420840.1414107054504599No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1404310.1397655385343426No Hit
TCGTGAATCATCGAATCTTTGAACGCACCTGTAGGCACCATCAATAGATC1400400.1393763913690662No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1372770.13662648441853256No Hit
CAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1344560.13381885231304746No Hit
TGTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGA1318990.1312739691887208No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1272990.12669576724429277No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1233080.12272367942685529No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG1223540.12177420015403262No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1210140.12044055002239488No Hit
TGGCGCCAGTGAAATACCACTACCTTTCTGTAGGCACCATCAATAGATCG1208060.12023353567360334No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG1204780.11990708996973977No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1166540.11610121078811089No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACCTGTAGGCACCATCAATAGATCGG1151560.11461030937229497No Hit
TATACAGTGAAACTGCGAATGGCTCCTGTAGGCACCATCAATAGATCGGA1145710.11402808151631881No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1118100.1112801650883697No Hit
TAGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGA1059570.10545490074473113No Hit
AGAGGTGCACAATCGACCGATCCTGCCTGTAGGCACCATCAATAGATCGG1056890.10518817071840358No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1044730.10397793298700696No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1044270.10393215096756267No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCCTGTAGGCACCATCAATAG1039700.10347731655699667No Hit
AGAGGTGCACAATCGACCGATCCTGTTGTAGGCACCATCAATAGATCGGA1037750.1032832406050046No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG1034440.10295380911726423No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGTACCATCAATAGATCGGA1019150.10143205460138806No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)