FastQCFastQC Report
Tue 4 Jul 2023
SRR6398748.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398748.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84570160
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA20625722.4388886103561824No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG13590601.6070207269325256No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG12222401.445237894784638No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA9592851.1343067105466043No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG5869700.694062775806502No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA5051740.5973430817678481No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC5022250.5938560362189217No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA4421260.5227919635010742No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA4034610.4770725277095373No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG3919470.4634577964615415No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA3713600.43911469482853055No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG3463490.4095404336470453No Hit
TGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG3126800.36972851890075653No Hit
TAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG3110350.36778338837244723No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG2674450.31624038549767436No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG2248900.26592121854800793No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT2229990.26368520527807915No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2011770.23788177768612478No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1977930.23388036631360282No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA1896870.22429542524218943No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA1858540.21976309374370345No Hit
TAGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCG1812290.21429426171122296No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG1723380.2037810972569994No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA1667260.19714518690753333No Hit
AGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGATCG1625700.19223092400440062No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1615290.19099999337827905No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT1611170.19051282390857485No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1586190.1875590633859508No Hit
TTTAAGGTAGCCAAATGCCTCGTCCTGTAGGCACCATCAATAGATCGGAA1533320.18130744934146983No Hit
AGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAGA1520380.1797773588225445No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGAT1504340.1778807087511718No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA1493610.17661193971963632No Hit
ATTGCTCTTCAACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAA1489420.17611649309874783No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC1453270.17184193573714415No Hit
TAAGGTGCCGGAATACACGCTCATCCTGTAGGCACCATCAATAGATCGGA1430210.1691152056470036No Hit
AGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGAA1405960.16624776398673008No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1403160.16591667793935827No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1383830.16363100176232373No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGAT1378670.1630208574750243No Hit
TAGACACCACAAAAGGTGTTAGTTCATCTCTGTAGGCACCATCAATAGAT1309580.1548513092561253No Hit
ATGATTAATAGGGACGGTCGGGGGCATCTGTAGGCACCATCAATAGATCG1298290.1535163230151155No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1267830.14991457979977807No Hit
TGTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGA1249810.14778380459490675No Hit
AGACCGTAAGGTCGGGTCGAACGGCCTCTCTGTAGGCACCATCAATAGAT1244050.14710271329745622No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1231070.14556789297785414No Hit
TTCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGG1186190.14026105661855198No Hit
ATCATACCGAAGCAGAATTCGGTAAGCCTGTAGGCACCATCAATAGATCG1160450.13721742988306987No Hit
TGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1147270.13565896056008409No Hit
TGGCGCCAGTGAAATACCACTACCTTTCTGTAGGCACCATCAATAGATCG1142110.13504881627278464No Hit
TCACGTTCTAGCATTCAAGGTCCCGCTGTAGGCACCATCAATAGATCGGA1116830.13205958224508502No Hit
TCGATCAACTTAGAACTGGTACGGACCTGTAGGCACCATCAATAGATCGG1112540.13155231112250468No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCAATAGAT1110010.13125315122970088No Hit
ACCACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGA1106260.1308097324162565No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1104390.13058861423461893No Hit
AAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGATCGGAA1077990.1274669457879706No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG1067050.12617334530288224No Hit
TAAGCGCGGGTAAACGGCGGGAGTCTGTAGGCACCATCAATAGATCGGAA1044890.12355303572796836No Hit
TTTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGAT1028350.1215972631481364No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA985050.11647725391556549No Hit
ATGAATGGATTAACGAGATTCCCCTGTAGGCACCATCAATAGATCGGAAG951970.1125657087559016No Hit
ACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGA928750.1098200594630541No Hit
ATGATTAATAGGGACGGTCGGGGGCATCCTGTAGGCACCATCAATAGATC917470.10848625567221346No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT908390.10741259091859351No Hit
TTTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCG906370.10717373598441815No Hit
ATGAAAGTGTGGCCTATCGATCCTTTCTGTAGGCACCATCAATAGATCGG903220.10680126418112489No Hit
ATCTAGACAGCCGGACGGTGGCCCTGTAGGCACCATCAATAGATCGGAAG887020.10488569490704523No Hit
AACAACGGATCTCTTGGTTCTCGCCTGTAGGCACCATCAATAGATCGGAA882540.10435595723125037No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCCTGTAGGCACCATCAATA875770.10355543846671214No Hit
TGGCGCCAGTGAAATACCACTACCTTTACTGTAGGCACCATCAATAGATC873060.103234994470863No Hit
TGGTCCTATAGTGTAGTGGTTATCACTTTCGGCTGTAGGCACCATCAATA866350.10244157040733988No Hit
GCGTAGTCGGTAGCGCTCTCCCCTGTAGGCACCATCAATAGATCGGAAGA855510.10115979442394339No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCCTGTAGGCACCATCAATAG847950.10026586209603955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)