FastQCFastQC Report
Wed 5 Jul 2023
SRR6398749.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398749.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109987608
Total Bases5.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG47983354.3626141955919255No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC40498213.682070256496532No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG32061392.915000206205048No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG29196982.654569958462957No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG20739871.8856551548970861No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA14372631.306749938593082No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG10974790.9978205908432884No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG10455200.9505798144096378No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA8889500.8082274141283261No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6584510.5986592598686208No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG5140150.4673390115002774No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG4697260.42707174793727676No Hit
TTGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATC4631720.4211128948272064No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT4514490.4104544213744516No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT4429690.4027444619033809No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC4405350.4005314853287836No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC4363220.39670105381326237No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATCCTGTAGGCACCATCAATAGAT3701860.3365706434855825No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA3630620.33009355017521613No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA3561650.3238228437516343No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC3510590.31918050258898256No Hit
TAGGCGTGGAGGTCAGTGACGAAGCCTCTGTAGGCACCATCAATAGATCG3322760.30210312419922797No Hit
TCGGGGCTGATCCGGGTTGAAGACATTGTCCTGTAGGCACCATCAATAGA3071440.27925327733284283No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA3028920.2753873872773013No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA2941670.26745467543943674No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2926870.26610906930533484No Hit
ACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCGGA2809750.25546059697925244No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG2763550.25126012377685314No Hit
ATCAGACACCACAAAAGGTGTTAGTTCCTGTAGGCACCATCAATAGATCG2218280.20168453886186885No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG2137560.19434553027101018No Hit
ATGAAAGTGTGGCCTATCGATCCTTTCTGTAGGCACCATCAATAGATCGG2002040.18202414221063884No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC1985050.18047942273642317No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA1945160.1768526505276849No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1937640.1761689371406277No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1903070.17302585578549903No Hit
ATCTAGACAGCCGGACGGTGGCCCTGTAGGCACCATCAATAGATCGGAAG1897530.17252216267854464No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG1828720.16626600334830446No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT1722580.15661582530279228No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT1708900.15537204882208186No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1636010.1487449386116298No Hit
TAGAATAAGTGGGAGCTTCGGCGCCCTGTAGGCACCATCAATAGATCGGA1618600.14716203301739228No Hit
ATGATGCCCTGACGAGTAGGCAGGCCTGTAGGCACCATCAATAGATCGGA1612070.1465683297703865No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1534930.13955481239304704No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1512380.1375045814252093No Hit
TAGACGTTCTGGGCCGCACGCGCGCTCTGTAGGCACCATCAATAGATCGG1460260.13276586576916918No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC1442450.13114659244157761No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1432900.13027831280774832No Hit
AGCTGGTTCCTGCCGAAGTTTCCCTCCTGTAGGCACCATCAATAGATCGG1430340.13004555931428202No Hit
ACAGGGATAACTGGCTTGTGGCCTGTAGGCACCATCAATAGATCGGAAGA1410660.12825626683326No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA1401430.12741708138611396No Hit
ATGATGCCCTGACGAGTAGGCAGGCGTCTGTAGGCACCATCAATAGATCG1358860.12354664536390317No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGAT1335120.12138822038933696No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1318500.11987714106847383No Hit
TGACCTATTCTCAAACTTTAAATATGTCTGTAGGCACCATCAATAGATCG1318450.11987259510180456No Hit
ATGGCTGAACGCCTCTAAGTCAGAATCCCTGTAGGCACCATCAATAGATC1301310.1183142377275811No Hit
AGGGATAACTGGCTTGTGGCAGTCCTGTAGGCACCATCAATAGATCGGAA1279680.11634765254645778No Hit
ATTGCTCTTCAACGAGGAATTCCTCTGTAGGCACCATCAATAGATCGGAA1232140.11202534743732222No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA1227330.11158802544373907No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCCTGTAGGCACCATCAATAG1175100.10683930866102662No Hit
TAAGGTAGCCAAATGCCTCGTCATCTCTGTAGGCACCATCAATAGATCGG1163850.10581646616044237No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1161590.10561098846699167No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT1159480.10541914867354875No Hit
AACAACTCACCGGCCGAATGAACTCTGTAGGCACCATCAATAGATCGGAA1142610.10388533951933931No Hit
TGTTTTCATGCCAGGTCGTACTGATCTGTAGGCACCATCAATAGATCGGA1112300.10112957452443189No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)