FastQCFastQC Report
Wed 5 Jul 2023
SRR6398750.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398750.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102705449
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG57914835.6389247662994No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG53292985.188914562848559No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA30898183.008426553882258No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC18887211.8389686412840667No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG16222941.579559814786458No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG10353041.008032202848361No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG9558390.9306604559997591No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC8601400.8374823423438809No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA7883010.7675357127351636No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG6787650.6608850909166465No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT6624820.6450310148588124No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA6496700.6325565063251902No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA6410270.624141178721686No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT6330710.6163947542841666No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG5704120.5553863067187409No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT5277360.5138344704573561No Hit
AAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG4050240.39435492852964404No Hit
AAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC4020200.3914300593729939No Hit
TAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG3117990.3035856451978512No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA2745070.26727598454878476No Hit
TAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC2283230.22230855541072606No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC2132290.20761215892255142No Hit
AAGAGGTGCACAATCGACCGATCCTGTAGGCACCATCAATAGATCGGAAG2057590.20033893235791217No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA1996190.1943606711655581No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1932030.18811368031700051No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1839040.17905963295092553No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG1682200.16378877813970708No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA1583000.15413008904717412No Hit
TTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATCG1582520.15408335345479088No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1578490.15369096921040673No Hit
TAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA1571560.15301622409537394No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC1526860.1486639720546862No Hit
AAGTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATA1486840.1447673920397349No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1484530.14454247700139064No Hit
CAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCG1472890.14340913888609746No Hit
TATAGATGGATACGAATAAGGCGTCCTGTAGGCACCATCAATAGATCGGA1461160.14226703784723244No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA1423030.13855447922729008No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC1403520.13665487212854696No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT1400090.13632090737464184No Hit
CAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATC1292970.12589108100778568No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA1257210.12240927937523548No Hit
TTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGG1232690.12002186953099245No Hit
TATAGATGGATACGAATAAGGCGTCCCTGTAGGCACCATCAATAGATCGG1218440.1186344066321155No Hit
TAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1205730.11739688709213471No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATCTGTAGGCACCATCAATAGA1205620.11738617685221354No Hit
CCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC1157940.11274377467547997No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGAT1152160.112181000250532No Hit
ACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCG1094110.10652891454668582No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1055600.10277935691610676No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)