FastQCFastQC Report
Wed 5 Jul 2023
SRR6398751.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398751.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177677405
Total Bases8.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA23243681.3081956031494268No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA22707601.2780240683951907No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC20379751.1470085349344223No Hit
TTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATCG18012331.0137659315769498No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGA16884380.9502829017566978No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG16763020.9434525453588204No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG15096480.8496567135252792No Hit
ACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGATCG14419030.8115286240250976No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA14371260.8088400435609693No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATC11482880.6462768859101696No Hit
TTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATC10528150.5925429854178701No Hit
TTGACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATAG10408230.5857936747781746No Hit
TTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGAT9764770.5495786028617426No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG9618220.5413305085134488No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG9081430.5111190136978869No Hit
TCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAAT7024570.39535527885495625No Hit
TGCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAAT7019670.39507949815003207No Hit
ACCTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGA6230480.3506624829420488No Hit
TACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAG6225190.3503647523442837No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTACTGTAGGCACCATCAATAGA6009570.33822927569208927No Hit
TCTTGTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAA5982600.33671135617947595No Hit
ATTAAGCCATGCATGTCTAAGTATAAGCAACTGTAGGCACCATCAATAGA5800990.3264900227465614No Hit
ATGGGGAAGCTCCGTTTCAAAGGCCTGCTGTAGGCACCATCAATAGATCG4858830.2734635841850572No Hit
TGTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGAT4842820.2725625129430498No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT4798980.27009511986062607No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGATC4746910.26716452775748273No Hit
ACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCAATAGATCGG4587930.2582168509271058No Hit
GCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA4507790.25370642935718246No Hit
TTTACGATCTGCTGAGATTAAGCCTTTGTTGCTGTAGGCACCATCAATAG4373240.2461337163270704No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG4367330.24580109102786593No Hit
AAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAGA4284430.24113533175476082No Hit
CTGAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGA4265540.24007216899638983No Hit
TTTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAAT4194750.23608798203688308No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT4192280.23594896604889068No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGAT4034120.22704744027525614No Hit
TAGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATC4031170.2268814090345365No Hit
TCAGGCCACCATCGAAAGGGAATCCGGTTAACTGTAGGCACCATCAATAG4004080.22535673570874137No Hit
TCTACATGGTATAACTGTGGTAATTCTAGCTGTAGGCACCATCAATAGAT3842920.21628636460556142No Hit
AGATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCG3547560.19966297909404968No Hit
TCTCATTAAATCAGTTATCGTTTATTTGATAGTTCTGTAGGCACCATCAA3524030.19833866889264845No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG3523500.19830883955109543No Hit
TCGTGAATCATCGAATCTTTGAACGCACATTGCTGTAGGCACCATCAATA3521520.19819740163359545No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3506960.19737793896753503No Hit
CCTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGAT3254750.1831831121126516No Hit
TGTTCCTTGTCTATGTTCCTTGGAACAGGCTGTAGGCACCATCAATAGAT3222570.1813719645443944No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA3206630.18047483302674305No Hit
TGCAGGCCACCATCGAAAGGGAATCCGGTTACTGTAGGCACCATCAATAG3178240.17887699339147822No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGA2974170.16739157125803364No Hit
TTTGACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATA2972870.16731840494856395No Hit
ATTAAGCCTTTGTTGTCTGATTTGTCTGTAGGCACCATCAATAGATCGGA2946690.1658449480393976No Hit
TCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATAGA2841300.159913411612467No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG2834150.15951099691038376No Hit
TTGACCTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAAT2800650.15762555739712655No Hit
TAGCGGAGAATTTGGACAAACTTGGTCATTTAGCTGTAGGCACCATCAAT2668820.15020593079913566No Hit
TGTTTTCATGCCAGGTCGTACTGATAACCGCTGTAGGCACCATCAATAGA2668640.15019580007936292No Hit
ACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATAGATC2622840.1476180947149695No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCTGTAGGCACCATCAATAG2598360.1462403168258789No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATAG2556960.1439102512781521No Hit
TCAAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATC2460870.1385021353728123No Hit
TTTGCTGGCTCCGGTGCGCTTGTGACGGCCCGTGCTGTAGGCACCATCAA2447350.13774120575432763No Hit
CTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGATC2342050.1318147346872834No Hit
TGCTGGCTCCGGTGCGCTTGTGACGGCCCTGTAGGCACCATCAATAGATC2328330.1310425487134957No Hit
TGACCTATTCTCAAACTTTAAATATGTAAGCTGTAGGCACCATCAATAGA2189170.1232103766936488No Hit
TCGATCAACTTAGAACTGGTACGGACAAGGCTGTAGGCACCATCAATAGA2188500.12317266790338366No Hit
TTTACCTATACTCTACCGTCAGGGTTGACTGTAGGCACCATCAATAGATC2082080.11718316124664246No Hit
TCGATCAACTTAGAACTGGTACGGACAAGCTGTAGGCACCATCAATAGAT2074900.11677905809126378No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC2074040.11673065576346074No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGGCTGTAGGCACCATCAATA2067230.11634737686539265No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGCTGTAGGCACCATCAATAG2008360.11303406868194636No Hit
TCTTGACCTATTCTCAAACTTTAAATATGCTGTAGGCACCATCAATAGAT1970980.11093025587581044No Hit
TATACAGTGAAACTGCGAATGGCTCATTACTGTAGGCACCATCAATAGAT1958560.1102312362114924No Hit
TCGCTTGTGACGGCCCGTGAAAATCCACAGGAAGGCTGTAGGCACCATCA1936960.10901554983876538No Hit
GTGACGGCCCGTGAAAATCCACAGGAAGGACTGTAGGCACCATCAATAGA1923990.108285575197364No Hit
TTGTTACGATCTGCTGAGATTAAGCCTTTGCTGTAGGCACCATCAATAGA1912130.10761807332789444No Hit
CCAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATA1893290.10655772465834921No Hit
TGCAGGCCACCATCGAAAGGGAATCCGGTTCTGTAGGCACCATCAATAGA1891300.10644572392308409No Hit
TTCAGAATCCATGCTAGAACGCGGTGATTTCTGTAGGCACCATCAATAGA1884820.10608101801126597No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAG1847450.1039777680228952No Hit
CAGGCCACCATCGAAAGGGAATCCGGTTAAGCTGTAGGCACCATCAATAG1845120.10384663148361492No Hit
TGGCGCCAGTGAAATACCACTACCTTTATAGCTGTAGGCACCATCAATAG1838400.1034684179454332No Hit
TCAGGCCACCATCGAAAGGGAATCCGGCTGTAGGCACCATCAATAGATCG1829630.10297482676539542No Hit
ATGTCGGCTCTTCCTATCATACCGAAGCAGCTGTAGGCACCATCAATAGA1821130.10249643166501672No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT1793170.10092279319365341No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)