FastQCFastQC Report
Tue 4 Jul 2023
SRR6398753.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398753.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77596602
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA32606054.201994566720847No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA19250302.480817394555499No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT18630492.4009414742155846No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT18567282.3927954989575446No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT16494462.1256678224131518No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA13917501.7935708061030817No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA13355351.7211256235163495No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA13051061.6819112775067135No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT8958851.1545415352079462No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG7051490.90873695732192No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA6463130.8329140495095391No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG6353910.8188386909004083No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA6150250.7925926962626534No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC5224060.6732330882220847No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA5070120.6533945906548846No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT4720620.6083539585921558No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG4378920.5643185251849043No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA4196880.5408587350255363No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT3785540.4878486818275882No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA3445450.444020731732557No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG3189310.41101155434615555No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA3013150.3883095293270703No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG2912920.37539272660418815No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG2852050.367548310942791No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2833680.3651809392375197No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG2825230.3640919740274194No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA2691040.34679869100453653No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2475350.3190023707481418No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2464440.31759638134669865No Hit
TTATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAA2336980.3011704043432211No Hit
TAGGCTGAGCTGTGATGGGGAAGCTCTGTAGGCACCATCAATAGATCGGA2245140.2893348345330895No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA2222330.2863952728239311No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA2213900.28530888504628077No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC1935190.24939107514012018No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC1896640.24442307409285785No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1784220.22993532629173632No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1783680.2298657356155879No Hit
AGGCTGAGCTGTGATGGGGAAGCTCTGTAGGCACCATCAATAGATCGGAA1744350.22479721470277783No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG1741690.22445441618693562No Hit
ATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAA1715250.22104705048811285No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT1687720.21749921472076833No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG1686030.21728142167874825No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG1657450.21359827070778176No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1638060.2110994499475634No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1626990.20967284108652076No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1598990.20606443565660257No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA1536730.19804088843993453No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA1526440.1967147994444396No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT1516110.19538355558404474No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG1479710.19069262852515115No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1465880.18891033398601653No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG1293130.16664776119964636No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA1283340.16538610801539996No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1264660.16297878610715455No Hit
TTTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAA1256140.16188079988347942No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1236810.15938971142061092No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA1185670.15279921664611035No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT1170760.15087774075467894No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1157530.14917276918904257No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1156260.1490091022284713No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1127010.14523960727043178No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1104590.14235030549404729No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT1089330.14038372453474185No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA1071910.13813878087084278No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATTAATAGATCGGA1043650.13449686881907535No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA1033860.13323521563482896No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA1031450.132924635024611No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1015090.1308162952805588No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAATA1004990.12951469189333834No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAAT992960.12796436627469848No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG981850.12653260254875592No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATTAAT967310.12465880915764843No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA960730.12381083388161765No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT955700.12316260962045736No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT955500.12313683529595794No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGTACCATCAA934350.12041120048014474No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT920590.11863792695458494No Hit
TCGCCCATTAAAGCGGTACGCGAGCTGGGTTCCTGTAGGCACCATCAATA915000.11791753458482628No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAGATC913180.1176829882318816No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT882390.11371503097519656No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT868880.11197397535526105No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAATA868540.11193015900361206No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA862990.11121492149875326No Hit
TTGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAAT842500.10857434195378814No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA833930.10746991214898817No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATTAATA833430.10740547633773963No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG832590.1072972241748421No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT829960.10695829180767477No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG825810.1064234745743119No Hit
AAGGCTGAGCTGTGATGGGGAAGCTCTGTAGGCACCATCAATAGATCGGA819770.10564508997442956No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG793600.10227251961368103No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)