FastQCFastQC Report
Wed 5 Jul 2023
SRR6398754.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398754.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125792562
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA39378383.1304219720081705No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA37047852.945154261187557No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT31684992.5188285774798036No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT29148712.317204573669467No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT27272402.1680455160774925No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA23513441.8692233965311875No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA20097531.5976723647619164No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC16447151.307481916140638No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG16129131.2822006121474814No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA14494311.1522390330200922No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG14430501.147166396054482No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA11945510.9496197398380359No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT10802740.8587741459626206No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT10355760.8232410434569255No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA9443800.7507439112337978No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG8465130.6729436037720576No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA8383700.6664702480580688No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG7813360.6211305243945981No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA7461870.5931884907471715No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT6922910.5503433501894969No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT5839150.464188812689895No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG5713180.45417470708641744No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC5645820.4488198594762701No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA5510730.4380807507521788No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG5375830.42735674625976694No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA4511590.3586531610668682No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT4369800.34738142943618555No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG4354360.34615401187233946No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA4172160.3316698486513058No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG3675860.29221600558544947No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG3640410.2893978739378883No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA3575310.2842226871887703No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3530040.2806239052512501No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG3501270.278336806591156No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA3484600.2770116090011745No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA3312870.2633597684416349No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT3311060.263215880760899No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA3181420.2529100249981394No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3143630.24990587281305235No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA2771890.22035404605242082No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2585960.2055733629147326No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA2505630.19918745275257213No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA2497710.19855784477940752No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG2486490.1976659001507577No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT2294400.18239552192283037No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT2198910.17480445306456194No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG2191140.17418676948482853No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2147610.17072631051110954No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT2106200.1674343829645508No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA2066000.1642386455250033No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA2034690.1617496271361418No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG2001040.1590745882097544No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1968610.15649653434994035No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG1897960.15088014504386993No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT1860610.14791097107951423No Hit
GCCAAGGACGTTTTCATTAATCAAGAACCTGTAGGCACCATCAATAGATC1860280.14788473741396568No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT1845900.1467415855636997No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT1802120.1432612526009288No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATTAATAGATCGGA1752960.1393532313937608No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA1742490.1385209087322667No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA1696820.13489032841226337No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1656760.13170572040658493No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1617950.12862048234616605No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT1617480.12858311924674848No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATTAAT1591200.12649396551761144No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATAGA1579020.12552570477100228No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAATA1552310.12340236778069598No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATACTGTAGGCACCATCAATAGA1543690.12271711263818602No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT1525120.12124087273140999No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCTGTAGGCACCATCAATAGATC1504900.11963346449689131No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA1494260.11878762752284193No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTCTGTAGGCACCATCAATAGA1475460.11729310354613813No Hit
TTTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAA1475210.11727322955708622No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATC1413320.11235322482739481No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1406510.11181185736562071No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT1406300.11179516321481711No Hit
ATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAA1372720.10912568900536423No Hit
TTATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAA1370440.1089444382252108No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAAT1356440.10783149483830372No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATTAATA1351560.10744355457201038No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACTATCAATAGATCGGA1257940.10000114315185027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)