FastQCFastQC Report
Tue 4 Jul 2023
SRR6398755.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398755.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41334454
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA12339592.9853037371680293No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA10277092.486325330437412No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT5962791.442571371572974No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT5579341.3498037254828623No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT5421521.3116225026221466No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA5338831.2916173998572715No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA5116371.2377978913184629No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA4587381.1098199095601942No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG3693370.8935330317898961No Hit
TTGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCAT3685820.8917064684101065No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCATC3026210.7321277305368543No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA2904800.7027551398162899No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA2785160.6738107632920469No Hit
TGGGCCAGCATCAGTTTTGGTGGCAGGATAAATCCACTGTAGGCACCATC2658050.6430591777019723No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT2532250.6126245190029606No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG2473730.5984668383426572No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT2394420.5792794553425091No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC2016890.4879440284852922No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1890630.45739808248102176No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1656020.4006391375098362No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG1647730.3986335467259347No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCA1645410.3980722716211517No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG1637000.3960376493663132No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1576640.38143481948497493No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1448560.35044856283815917No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATGGCCCTGTAGGCACCAT1432230.34649786350147505No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1400870.33891097242992496No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1327360.32112677719173455No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA1304470.31558902411049145No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1300990.31474711145331685No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA1275080.30847873302015794No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1170440.28316329036304677No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1170280.28312458173513066No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC1138000.27531511605306314No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAAT1133200.27415385721558094No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG955780.23123082743514647No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA855070.2068661654512238No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT839070.2029953026596166No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG801880.19399796595837457No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG798770.19324556700325593No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG748140.18099670555706385No Hit
TTATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA737060.17831613307387587No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA706570.17093972016661935No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA699430.16921234764586465No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA684140.165513254390635No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA633960.15337326096045686No Hit
TGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTCTGTAGGCACCATCA610950.14780647640827674No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA608860.14730084495612306No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA603240.145941204400571No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAATA601110.1454258957914383No Hit
TCAGATTTAGGCTCTGATACTGTAGGCACCATCAATAGATCGGAAGAGCA575180.13915267877978987No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT568040.13742530625903512No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAATA554140.13406249420882638No Hit
GGGAATACTGCCAGCTGGGACTGAGGACTGCGACGCTGTAGGCACCATCA521690.126211900609598No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT495580.11989513639154396No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATC492350.11911370596548825No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG466190.11278484530121047No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT456820.11051797127887548No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAATA446500.10802126477828884No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAATA443000.10717451354262475No Hit
TTATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAA428720.10371976850111532No Hit
ATTGCTCTTCAACGAGGAATTCCTAGTAAGCGCAAGCTGTAGGCACCATC420330.10168998482476628No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA418620.10127628636391325No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGA414830.10035937574015129No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)