FastQCFastQC Report
Tue 4 Jul 2023
SRR6398757.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398757.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102471632
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA32165273.138943859116053No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA29886282.916541819105604No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT26716252.607184981693275No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA11703661.1421365866408764No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT11319781.1046745112832788No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT10255411.0008047885877331No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG9623630.9391506519579975No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA9246030.9023014291408962No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA9076300.8857378205901902No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG6643150.6482916169423358No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA6077240.5930656008289201No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG5630750.5494935417833494No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG4714790.4601068518163154No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT4414240.43077678317839224No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA4312620.4208598922285145No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT4189460.4088409560999282No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT3431970.33491903398200973No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA3362420.3281317896839976No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA3165930.3089567266772915No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3114280.3039163072956621No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT2940510.28695844328896797No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA2930330.28596499761026545No Hit
TTTTGATGATTCATAATAACTTTTTGAATCGCATCTGTAGGCACCATCAA2892930.2823152070028513No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG2862420.27933779760626826No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG2847420.27787397784393636No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2514310.24536644444191152No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATAGATC2506220.24457695765009382No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2484820.24248857478916702No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA2472900.24132532601803391No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA2437400.23786095258051515No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA2341560.228508120179056No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG2232700.21788469222389276No Hit
ATGATTAATAGGGACGGTCGGGGGCACTGTAGGCACCATCAATAGATCGG2128900.20775505946855613No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG2018070.19693938318460663No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1998640.19504324865246608No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1874720.18295014565592163No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA1825910.17818687614929368No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1786710.1743614271703997No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA1741580.16995728144546385No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT1726220.168458330008836No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1653690.16138027351804057No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1625740.1586526893608955No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG1620260.1581179072077236No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1618240.15792077947972957No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC1617880.15788564780543363No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1586140.15478820518833933No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT1568940.15310969186086545No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1526060.1489251191002794No Hit
AATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGG1493070.1457056915029908No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG1475820.14402229877630912No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1456750.14216129591846455No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1445850.14109758689117002No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT1415180.13810456341712213No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATA1397100.1363401726635914No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT1392790.13591956845188138No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1390720.1357175613246796No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATCG1374450.13412980482247028No Hit
TGATGTTGACGCAATGTGATTTCTGCCCACTGTAGGCACCATCAATAGAT1309040.12774657477886173No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1298110.12667993811204256No Hit
TACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGG1295200.12639595707815018No Hit
TCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAA1278990.12481405585499018No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGA1215220.11859087010539658No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG1202650.11736418914456245No Hit
GAAAAGAAACCAACCGGGATTGCCTTAGTAACGCTGTAGGCACCATCAAT1202640.11736321326472092No Hit
CATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAG1196960.11680891351471791No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1182800.11542706765907661No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1148610.11209053448080147No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1141840.11142986382806902No Hit
ACCCACTAATAGGGAACGTGAGCTGGGTTTACTGTAGGCACCATCAATAG1116950.10900089890243966No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1113510.10866519623694487No Hit
GATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCG1107790.10810699296757566No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACCTGTAGGCACCATCAA1106150.1079469486735607No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1104660.10780154257716906No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAAACTGTAGGCACCATCAA1100050.10735166197021241No Hit
TTTGATGATTCATAATAACTTTTTGAATCGCATCTGTAGGCACCATCAAT1099030.10725212222637383No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1048900.1023600365806607No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGACTGTAGGCACCATCAATA1026430.10016723457668752No Hit
GGAATGCAGCTCTAAGTGGGTGGTACTGTAGGCACCATCAATAGATCGGA1025560.10008233303047227No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)