FastQCFastQC Report
Fri 7 Jul 2023
SRR6398758.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398758.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77145592
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA39108975.069501573077565No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA17000892.2037409473764877No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT11433121.4820185708082971No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA10002901.2966262544203433No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT9035921.1712814388669155No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT8562621.1099299101885174No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA7934241.0284761312091557No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA6456800.8369629207071221No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG5556620.72027705743706No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT5443020.705551653553971No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG5314550.6888987254125939No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA5299420.6869374986454184No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA5219950.6766361971789652No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG5127480.6646497702681444No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG4965710.6436803284885027No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG4241110.5497540287201373No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA3844900.498395293926839No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT3523830.4567765842019853No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA3444000.4464286177232265No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG3132030.40598949580943006No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA2916200.3780125246819028No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA2866990.3716336767498005No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG2719450.35250879920657036No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA2340590.3033990587563318No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT2224670.2883729247939403No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA2101490.2724057130833865No Hit
CACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAA2064450.2676044018172808No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGAT2062690.2673762617571202No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA2023500.2622962566675229No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA2007310.26019762736411434No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1841990.23876801671312597No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAGAT1823840.2364153223427205No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT1780580.23080774336400192No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1694290.21962239916442663No Hit
AAGAGGTGCACAATCGACCGATCCTCTGTAGGCACCATCAATAGATCGGA1655440.21458646658645122No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1613330.2091279563970421No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTCTGTAGGCACCATCAATAG1611070.2088350038197905No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1496240.1939501611446575No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA1478310.19162598428177205No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA1460660.1893381024284576No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1440590.18673652799242244No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1437210.18629839537688686No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1432480.18568526896520543No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG1405980.1822502055593792No Hit
GCCCAGTTGGTTAAGGCACCGTCTGTAGGCACCATCAATAGATCGGAAGA1388890.18003491372520675No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1369880.17757074182540464No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG1364140.17682669412919924No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1334380.1729690531119393No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1323270.17152891898217593No Hit
ACCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA1318820.1709520875800655No Hit
GTCTAGTGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1279140.1658085662237189No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1179220.15285643280824132No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1176480.15250126021458232No Hit
ACCCACTAATAGGGAACGTGAGCTGGGTTTACTGTAGGCACCATCAATAG1168380.15145129743770713No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG1155250.14974932073889588No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG1152540.14939803689626233No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA1147480.1487421342233008No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTGTAGGCACCATCAAT1133170.14688719998415464No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCACTGTAGGCACCATCAATAGATC1130250.14650869488434284No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA1123850.14567909466557727No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT1118780.1450218957422739No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGATCG1116010.14466283439758942No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1102690.14293622894228358No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA1100910.1427054963814394No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT1093810.14178515863874633No Hit
TACCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAG1080650.1400792931889096No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1078700.13982652437225448No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT1050560.1361788759103696No Hit
AGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCGG1023940.1327282575004415No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG1008260.13069573696446582No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA997420.12929060159393163No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGATC977040.1266488433973No Hit
TACCCACTAATAGGGAACGTGAGCTGGGTTTACTGTAGGCACCATCAATA973640.12620811828108078No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG934290.1211073731860143No Hit
GTCTAGTCGGTTATGGCATCTGCTGTAGGCACCATCAATAGATCGGAAGA930850.12066146306842782No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA929940.12054350428732208No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA928680.1203801767442526No Hit
TTTTGATGATTCATAATAACTTTCGAATCGCATCTGTAGGCACCATCAAT897480.1163358756777704No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA874860.11340375740457082No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG872370.11308099106945735No Hit
GTTTAGTGGTAAAATCCAACGTCTGTAGGCACCATCAATAGATCGGAAGA859420.11140234687679887No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT857650.11117291056629652No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA855180.11085273673186669No Hit
CATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAG852540.11051052664162588No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA844040.10940871385107785No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA807530.10467610385308859No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG802190.10398390617055607No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC783960.10162084179741598No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA783420.10155084427895764No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG776720.10068235654993743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)