FastQCFastQC Report
Fri 7 Jul 2023
SRR6398759.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398759.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112482246
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length50-51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA61461155.464075637323245No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA49808154.428089922742118No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT46205894.107838493907741No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT26760802.3791132335675447No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT15168101.3484883649993973No Hit
TATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA14989401.3326014133821615No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA11917621.0595112049949642No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT11398961.0134008170498303No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCA10842900.9639654599357841No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT9585080.8521415904159666No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATA9056620.8051599538650749No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA9011920.8011859933877921No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA8163350.7257456434502562No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA5977710.5314358676657293No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG5793080.5150217217390912No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG4884540.4342498637518315No Hit
TTTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGAT4764340.4235637328934559No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG4641440.4126375641539021No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAATAG4385130.38985085699657884No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA4107810.36519629951201366No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAAT3597190.3198006910352768No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG3487040.3100080345123976No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA3346710.29753228789546043No Hit
CCCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGA3168990.2817324611388005No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3162160.28112525420233875No Hit
GTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA2980090.26493869974822515No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGAACTGTAGGCACCATCAAT2962180.2633464484697434No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT2918530.25946583605736323No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA2902080.2580033830405556No Hit
CCCACTAATAGGGAACGTGAGCTGGGTTTCTGTAGGCACCATCAATAGAT2843630.2528070074276433No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA2737050.24333173432543304No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2690070.2391550751929331No Hit
CATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA2415330.2147298872392715No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC2408520.21412445836118885No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2337570.20781679626134067No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG2250210.200050237261443No Hit
TTGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAAT2206260.1961429539733764No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA2086330.18548082690311857No Hit
TTTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATC2067980.18384945834029665No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAA2049110.1821718602596182No Hit
TTTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCG1977630.17581707961272394No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG1951770.17351805012855095No Hit
TTTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGA1927320.17134437376010433No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG1919720.1706687115760473No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1915930.17033176951320833No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAGA1853130.16474866620284234No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1843840.1639227580857516No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAG1821380.16192599852602518No Hit
TTTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGA1769940.15735283237498654No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1730510.1538473902805959No Hit
GATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1696740.1508451387074899No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1676310.14902885207324185No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC1665480.1480660334609606No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAATA1655960.1472196776725102No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAA1522990.13539825653908086No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA1492910.13272405673691828No Hit
AATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAA1478540.13144652179153676No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1475950.13121626323144364No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG1463940.13014853917479563No Hit
TTTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCA1460170.12981337517033578No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1432110.12731875926446204No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATTAAT1392120.1237635315354567No Hit
TTTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1333630.11856359980578625No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAA1329050.11815642443697293No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA1325780.11786571189199051No Hit
TTGCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATA1319550.11731184670690165No Hit
CTTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAAT1277880.11360726207405211No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGCTGTAGGCACCATCAATAGAT1272380.11311829601980032No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1231790.10950972653942205No Hit
AAGAGGTGCACAATCGACCGATCCTGATTGTAGGCACCATCAATAGATCG1224620.10887229261051562No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1181260.10501746204463235No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1174440.10441114413736012No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAA1168120.10384927768956533No Hit
AATACTGAATATCTAAATATTATTATTACTTCTGTAGGCACCATCAATAG1146030.10188541220985221No Hit
TTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAA1131570.10059987600176477No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)