FastQCFastQC Report
Fri 7 Jul 2023
SRR6398764_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398764_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116793359
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC33542002.871909866039558No Hit
TCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAA17497761.4981810738057462No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGAT17169321.4700596118654314No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG14829861.2697519899226462No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA12367071.0588846922366537No Hit
TCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA11508500.9853728070274955No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG10641860.9111699578740603No Hit
ATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCG9944320.8514456716669995No Hit
ACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAG9387410.8037623098073581No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA8581390.7347498242601277No Hit
TTCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA7868690.6737275190449827No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG7740350.6627388805557001No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGA7684370.6579457998121281No Hit
ATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAA7223240.6184632466988128No Hit
TGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGAT7178870.6146642293248883No Hit
TCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA6280070.5377077989511373No Hit
TGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGG6271930.5370108415153981No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6059850.5188522748112758Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC5382060.4608190094096018No Hit
TTCCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGA5094770.43622086423595374No Hit
CCAACGGGGCCTTTCCTTCTGGCCTGTAGGCACCATCAATAGATCGGAAG4987600.42704482880743244No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG4863150.4163892572008311No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGG4703850.40274978305915493No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATC4622960.3958238755681305No Hit
TCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG4440870.380233091848998No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT4354540.37284140445005953No Hit
TCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAA3777450.32343020462319266No Hit
AACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGATCG3578760.30641810721446927No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGAT3574090.30601825571263863No Hit
TCCAACGGGGCCTTTCCTTCTGGCTCTGTAGGCACCATCAATAGATCGGA3336280.28565665278965047No Hit
TTCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGG3233710.2768744753714978No Hit
TGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCGG3137810.2686633920683795No Hit
TCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGA2979280.25508984633278675No Hit
ACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGATCGG2809570.24055905438938527No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG2797820.23955300403681346No Hit
TTGCGTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATC2619310.22426874459531557No Hit
GATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGA2570980.22013066684724772No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG2495870.21369965050838208No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCTGTAGGCACCATCAATAGATC2400720.2055527831852152No Hit
AGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2342390.20055849237112874No Hit
AGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACAC2318150.19848303189909966No Hit
GATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATC2198030.18819820055008435No Hit
CTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA2087040.17869509173034404No Hit
TAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGATCGGAAGAGCACA2056020.17603911879955433No Hit
CTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATC2044880.17508529744400964No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA2007420.171877923298704No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGAT1958620.16769960353653326No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG1943240.16638274784099666No Hit
CCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1860060.15926076755785404No Hit
ATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCGGAAG1854070.15874789593130892No Hit
TTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGATCGGAAGA1798080.15395395897467082No Hit
TCCAACGGGGCCTTTCCTTCTGGCTAACCCTGTAGGCACCATCAATAGAT1624450.13908753150939002No Hit
CCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGA1609920.137843453924465No Hit
TCCAACGGGGCCTTTCCTTCTGGCTGTAGGCACCATCAATAGATCGGAAG1566560.13413091407020838No Hit
GCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAA1554510.133099177325656No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG1544100.13220785952393063No Hit
TTCCAACGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAG1542260.13205031631978323No Hit
TTTCCACGTTCTAGCATTCAAGGTCCTGTAGGCACCATCAATAGATCGGA1475400.12632567576038292No Hit
TCAAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGAT1473900.12619724380048014No Hit
TTTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGG1449100.12407383539675403No Hit
TTTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAA1448890.12405585492236765No Hit
TCTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCA1400830.11994089492708229No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1387240.11877730137036303No Hit
TTGTGGCGTCGCTGAACCATAGCCTGTAGGCACCATCAATAGATCGGAAG1380810.11822675636891306No Hit
GTCTAGTCGGTTATGGCATCTGCCTGTAGGCACCATCAATAGATCGGAAG1379730.11813428535778306No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG1340910.11481046623549888No Hit
TTGATGTATTTATTAGATAAAAAATCAATGCTGTAGGCACCATCAATAGA1338990.11464607332682332No Hit
TTGCCTTGTTGTAGACGGCCTTGGCTGTAGGCACCATCAATAGATCGGAA1322590.11324188389855283No Hit
AAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1319110.11294392175157834No Hit
ATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATAGATCGGAAG1315080.11259886788597287No Hit
CCAACGGGGCCTTTCCTTCTGGCTAACCTTGAGTCTGTAGGCACCATCAA1314120.11251667143163507No Hit
TCGGGGCTGATCCGGGTTGAAGACCTGTAGGCACCATCAATAGATCGGAA1308050.1119969501005618No Hit
TCGGGGCCTTTCCTTCTGGCTAACCTCTGTAGGCACCATCAATAGATCGG1280000.10959527245037964No Hit
TGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCGGAAG1261030.10797103626414238No Hit
TTTTTCCACGTTCTAGCATTCAAGGTCCCACTGTAGGCACCATCAATAGA1257710.10768677352622422No Hit
ACGCGAAACTCAGGTGCTGCAATCTCTGTAGGCACCATCAATAGATCGGA1249200.10695813620704238No Hit
TTGATGTATTTATTAGATAAAAAATCACTGTAGGCACCATCAATAGATCG1238950.10608051781437332No Hit
AACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACA1230450.10535273670825754No Hit
TGATGTATTTATTAGATAAAAAATCCTGTAGGCACCATCAATAGATCGGA1202240.1029373596490191No Hit
TTTCGGTGCCCGAGTTGTAATTTGCTGTAGGCACCATCAATAGATCGGAA1193560.10219416670771496No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)