FastQCFastQC Report
Tue 4 Jul 2023
SRR6398765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70560868
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT40392185.724444886363926No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA34738444.923187736295988No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT33570264.7576313828792465No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT24970693.5388864547414576No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA14112532.0000505095827337No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA11101191.5732785486709149No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA10256201.4535252032330441No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG7971891.1297891063358234No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6560180.9297192886005881Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5267300.7464902500915946No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA5058220.7168590953274555No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA4138020.5864468674053159No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA3925590.556340945238939No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC3629430.5143686724488706No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG3439680.48747699645644954No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA3438190.48726583125366313No Hit
ATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGA2801890.3970883691510144No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA2787610.39506458452296817No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2703110.3830891082575685No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC2615800.3707153942607395No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG2611680.37013150121679345No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA2345180.33236269145668673No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT2033850.28824050180335087No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2007070.2844451970177011No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA1899080.2691406800721329No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG1867070.2646041712525418No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1812370.25685199904286893No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1796340.2545802015927582No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG1791870.25394670598439917No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1783580.25277183381587653No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1742910.24700801583109774No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1728120.2449119531806213No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1724190.24435498724307078No Hit
TTGCTCGAATATATTAGCATGGACTGTAGGCACCATCAATAGATCGGAAG1709040.24220790481205534No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCCTGTAGGCACCATCAATAGATCGG1692440.23985532604275786No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA1588570.2251347021411358No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1578730.2237401614730703No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1570470.22256954095292592No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1556220.2205500079732579No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1554000.22023538599326756No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1503870.21313088155321447No Hit
TATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGA1482750.21013772109492757No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA1441720.20432288332960982No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1365300.19349251769408504No Hit
AGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGA1329110.18836361253379139No Hit
TTGCTCGAATATATTAGCATGGCTGTAGGCACCATCAATAGATCGGAAGA1296040.18367687880483557No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1292760.18321203191548038No Hit
TGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGAA1265130.17929626375911362No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1227810.1740072131765726No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1217400.17253189118932039No Hit
ATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAG1148630.16278569589024897No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1121170.1588940204080256No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1104610.15654711050323247No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCG960090.1360655030490838No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA956800.13559923894360257No Hit
ATGATTAATAGGGACGGTCGGGGGCCTGTAGGCACCATCAATAGATCGGA951420.13483677666777No Hit
ATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCA948120.1343690953461627No Hit
ATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAA927180.1314014447781453No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG921770.1306347308539345No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT916190.1298439242555803No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT915760.12978298396215873No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA885210.12545338869697578No Hit
GCCAAGGACGTTTTCATTAATCAAGAACGACTGTAGGCACCATCAATAGA848470.12024653664974756No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT840510.11911843261338566No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT837790.11873294982709112No Hit
ATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAG834800.11830920220539237No Hit
ACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGGAAGAG827730.11730723040425182No Hit
ACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCAC826350.1171116545788524No Hit
CACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAG816620.11573270328817384No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT801270.11355727653463672No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA779490.11047057981202839No Hit
TTACCCAATCCTAATTCAGGGAGGTAGCTGTAGGCACCATCAATAGATCG741600.10510074791030065No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG736280.1043467889312246No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG734590.10410727940591659No Hit
GCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCA730290.103497876471701No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)