FastQCFastQC Report
Tue 4 Jul 2023
SRR6398767.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398767.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85159331
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT82511359.689055683164067No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT57880236.79669853207278No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT38621374.535189455633464No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA34094034.003557754581233No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA20819312.4447479513431123No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA15573831.8287872646627532No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA10587011.243200231340474No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT7665320.9001151030648656No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA7426040.8720171838832318No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC5686240.6677177865570597No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA5331690.6260840635302783No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG5089220.5976115523970005No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA4794680.5630246202850043No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG4530870.5320462181648656No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG4489400.5271765227934917No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA4157610.4882154370141775No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA4094670.48082458515321125No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA3190850.3746917645466238No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA2821860.3313623964471961No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC2794310.3281272841375421No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2793040.327978151918549No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC2677540.3144153398762609No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG2437670.28624813879761457No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2407220.2826724883501022No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA2195230.25777915047265926No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA2068740.24292581631483226No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2062740.24222125465029778No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1954760.2295414932275595No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1870500.2196470989186141No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC1843140.21643429772833703No Hit
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1805080.21196502823630686No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1771270.20799482325665522No Hit
TTGCTCGAATATATTAGCATGGAATAATAGCTGTAGGCACCATCAATAGA1770630.20791967001243822No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1761550.20685343336010942No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1723520.2023876866764019No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAG1685380.1979090230288446No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA1626360.19097848478870744No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC1620030.19023517223262357No Hit
TTGTTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT1454430.1707892702914728No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG1400930.1645069287827073No Hit
TTTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAA1385940.16274669889081209No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATTAAT1345080.1579486339553325No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1317180.15467242221524732No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACTATCAAT1293380.15187766094592736No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCATCATCAAT1276340.1498767058186495No Hit
TTGCTCGAATATATTAGCATGGAATACTGTAGGCACCATCAATAGATCGG1270870.14923438043448226No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1258480.14777946059721864No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCCTGTAGGCACCATCAATAGAT1253880.1472392966544089No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1244640.14615427169102585No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG1230270.14446684650446584No Hit
ATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGA1198010.1406786532881523No Hit
ATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGAT1165290.13683644367755776No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGTACCATCAAT1160230.13624226334046705No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGA1140450.13391955838638517No Hit
TTGCTCGAATATATTAGCATGGAATAACTGTAGGCACCATCAATAGATCG1108270.13014075932559874No Hit
TTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATAG1095960.12869523364386223No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATTAAT1081360.12698080026016173No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1079770.12679409141906012No Hit
TTGCTCGAATATATTAGCATGGAATAATACTGTAGGCACCATCAATAGAT1068850.12551178918960743No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1035460.12159090352647321No Hit
TATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAA998830.11728955456449042No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT996740.11704413225134425No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA993500.11666366895249564No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT991570.11643703495040374No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG986360.11582524057169964No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC926950.10884890582336772No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATAG918470.10785312533749236No Hit
ATTTCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT899720.10565137013582222No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCATCTGTAGGCACCATCAATA897740.10541886478652586No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACTATCAAT897260.1053624998533631No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATCTGTAGGCACCATCAATAG890360.10455225393914849No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)