FastQCFastQC Report
Wed 5 Jul 2023
SRR6398768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6398768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98125685
Total Bases4.9 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA73808697.521852204140027No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAAT46816594.7710841458074915No Hit
TTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT28837022.938784070653876No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAAT27620452.8148032800993947No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAAT19637742.001284373199535No Hit
TGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT16763241.7083437430271187No Hit
GCATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACAC13044761.3293930126449562No Hit
TTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATA11827541.2053459805146838No Hit
TTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC8069690.8223830488419012No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATA7333070.7473140187505443No Hit
ATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACG7188260.7325564147654103No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATA7183500.7320713226103849No Hit
GCATGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCAC6711290.6839483464497598No Hit
TATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACA6564280.6689665402081015No Hit
CATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6428410.6551200126653893No Hit
TGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC6381630.6503526574107483No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6181290.6299359846507059Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG5939050.6052492780050401No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATA5287600.5388599325446747No Hit
TCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACA5050190.5146654517621966No Hit
TGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAG4429000.45135990643020735No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATACTGTAGGCACCATCAA4356880.4440101488208719No Hit
GCATGGAATAATAGACTGTAGGCACCATCAATAGATCGGAAGAGCACACG4292230.43742165978255343No Hit
TTGCTCGAATATATTAGCATGGAATCTGTAGGCACCATCAATAGATCGGA4104430.4182829398846999No Hit
ATTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3789530.3861914441667337No Hit
TCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT3682220.3752554695541743No Hit
TTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC3406170.3471231818661954No Hit
ATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAG3244300.3306269912918315No Hit
TCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2836390.28905683562871437No Hit
TTGCTCGAATATATTAGCATGGAATAATAGACTGTAGGCACCATCAATAG2682770.27340140351631687No Hit
AAGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCG2649060.2699660134856638No Hit
TTTTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT2511180.2559146466085816No Hit
GCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAG2427820.24741941928863986No Hit
AGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCAC2292940.23367378276136366No Hit
AGAGGTGCACAATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGG2095980.213601566195436No Hit
GCATGGAATAATAGAATCTGTAGGCATCATCAATAGATCGGAAGAGCACA2028880.20676339737144256No Hit
GCATGGAATAATAGAATCTGTAGGCACCATCAATAGATTGGAAGAGCACA1955270.19926179368837016No Hit
TTTGAGCGTCATTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1925370.19621468120197072No Hit
TTGCTCGAATATATTAGCATGGAATAATCTGTAGGCACCATCAATAGATC1847270.1882555010953554No Hit
TCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1837530.18726289655965203No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTCTGTAGGCACCATCAATA1772680.1806540254980131No Hit
GATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAATAGA1670730.17026428911044034No Hit
GGAATAATAGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1661680.16934200255519236No Hit
TGGAATAATAGAATACTGTAGGCACCATCAATAGATCGGAAGAGCACACG1647120.1678581912574674No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCG1596470.16269644385157667No Hit
AAGAGGTGCACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGG1553730.1583408054680077No Hit
AATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1509630.15384656932586No Hit
TGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC1449810.14775030615072904No Hit
AATCGACCGATCCTGACTGTAGGCACCATCAATAGATCGGAAGAGCACAC1437780.1465243274480071No Hit
TTCGAATCGCATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1425120.14523414537182594Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1382820.14092334743956184No Hit
TCGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGAT1353190.1379037506846449No Hit
ACAATCGACCGATCCTGCTGTAGGCACCATCAATAGATCGGAAGAGCACA1318800.1343990617747025No Hit
TTGCTCGAATATATTAGCATGGAACTGTAGGCACCATCAATAGATCGGAA1306470.13314251003699998No Hit
CGAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATC1289510.13141411445943027No Hit
TGCTCGAATATATTAGCATGGAATAATAGAACTGTAGGCACCATCAATAG1279570.1304011279004065No Hit
GCATGGAATAATAGAATCTGTAGGCACTATCAATAGATCGGAAGAGCACA1278730.13031552340246083No Hit
ACGTTCTAGCATTCAAGGTCCCCTGTAGGCACCATCAATAGATCGGAAGA1276740.13011272227042286No Hit
TTTTGATGATTCATAATAACTTTTCGAATCGCATCTGTAGGCACCATCAA1276130.13005055709929567No Hit
TGCAAGAGTTCGAATCTCTTAGCAACCACCACTGTAGGCACCATCAATAG1244970.1268750378659777No Hit
ATGGAATAATAGAACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1216050.1239277972938482No Hit
TTCAATGAAGCGGAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1182510.1205097319830175No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCTGTAGGCACCATCAATAGATCGG1182430.12050157917368934No Hit
ATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCGGA1175650.11981062858312785No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGATC1171720.11941012182488205No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCACTGTAGGCACCATCAATA1135650.11573422391904832No Hit
GCATGGAATAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1099270.11202673387706796No Hit
TGAGCGTCATTTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1088120.11089043607695578Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCATGGAATAATAGAATCTGTAGGTACCATCAATAGATCGGAAGAGCACA1076650.10972152703953097No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATCTGTAGGCATCATCAAT1058910.1079136415710117No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTCTGTAGGCACCATCAATAGAT1023820.10433761557944793No Hit
GCATGGAATAATAGAATCTGTAGGCACCATTAATAGATCGGAAGAGCACA1009020.1028293458537385No Hit
GAATATATTAGCATGGAATAATAGAATCTGTAGGCACCATCAATAGATCG997720.10167776153613603No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)